Q8K9R7 (GRPE1_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GrpE 1

Alternative name(s):
HSP-70 cofactor 1

Gene names

Name:	grpE1
Ordered Locus Names:	BUsg_243

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GrpE family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Protein GrpE 1 HAMAP-Rule MF_01151		PRO_0000113758

Sequences

	Sequence		Length	Mass (Da)
	Q8K9R7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 67C384A008834596	FASTA	202	24,068
	10 20 30 40 50 60 MKNKKEEKIN NLKKEEKINN LKKEEKINNL KKEEKEKNDD VQNKIIDLLE IKLKKSQEKI 70 80 90 100 110 120 INMQLKNHEE ILKLNYRLNS DIEKSRKFSL EKVIIEFLPI IDNIERALSV IKDKKEAFYL 130 140 150 160 170 180 EIINKMNFIF SLLEEILSEF NVSKINEKNI SFDPEIHQAM SINYNDEIED NHVVDVMQSG 190 200 YMLHKARLLR PAMVIVSKRK NN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67802.1.
RefSeq	NP_660591.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9R7.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg243.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67802; AAM67802; BUsg_243.
GeneID	1005444.
KEGG	bas:BUsg243.
PATRIC	21247289. VBIBucAph100086_0254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0576.
KO	K03687.
OMA	SINYNDE.
ProtClustDB	CLSK315757.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-304-MONOMER.
Family and domain databases
Gene3D	2.30.22.10. 1 hit. 3.90.20.20. 1 hit.
HAMAP	MF_01151. GrpE.
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
PANTHER	PTHR21237. PTHR21237. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
SUPFAM	SSF51064. GrpE_head. 1 hit.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE1_BUCAP

Accession

Primary (citable) accession number: Q8K9R7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program