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Q8K9R4 (TADA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-specific adenosine deaminase

EC=3.5.4.33
Gene names
Name:tadA
Ordered Locus Names:BUsg_246
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length151 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2) By similarity. HAMAP-Rule MF_00972

Catalytic activity

Adenine34 in tRNA + H2O = hypoxanthine34 in tRNA + NH3. HAMAP-Rule MF_00972

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00972

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00972

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Contains 1 CMP/dCMP deaminase zinc-binding domain.

Ontologies

Keywords
   Biological processtRNA processing
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA wobble adenosine to inosine editing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functiontRNA-specific adenosine-34 deaminase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 151151tRNA-specific adenosine deaminase HAMAP-Rule MF_00972
PRO_0000171730

Regions

Domain5 – 10399CMP/dCMP deaminase zinc-binding

Sites

Active site571Proton donor By similarity
Metal binding551Zinc; via pros nitrogen; catalytic By similarity
Metal binding851Zinc; catalytic By similarity
Metal binding881Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9R4 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 8B3F699B11203951

FASTA15117,502
        10         20         30         40         50         60 
MKSNRDSYWM KIALKYAYYA EENGEVPIGA ILVFQEKIIG TGWNSVISQN DSTAHAEIIA 

        70         80         90        100        110        120 
LREAGRNIKN YRLVNTTLYV TLQPCMMCCG AIINSRIKRL VFGASYKDLK KNPFLKKIFI 

       130        140        150 
NLEKNKLKIK KHIMRNECAK ILSNFFKNKR F 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67805.1.
RefSeqNP_660594.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9R4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67805; AAM67805; BUsg_246.
GeneID1005448.
KEGGbas:BUsg246.
PATRIC21247295. VBIBucAph100086_0257.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0590.
KOK11991.
OMAKDMGACG.
OrthoDBEOG64FKGZ.
ProtClustDBCLSK493053.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-260-MONOMER.

Family and domain databases

HAMAPMF_00972. tRNA_aden_deaminase.
InterProIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
IPR028883. tRNA_aden_deaminase.
[Graphical view]
PfamPF00383. dCMP_cyt_deam_1. 1 hit.
[Graphical view]
SUPFAMSSF53927. SSF53927. 1 hit.
PROSITEPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTADA_BUCAP
AccessionPrimary (citable) accession number: Q8K9R4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names