Q8K9R0 (LEP_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	BUsg_250

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Signal peptidase I		PRO_0000109504
Regions
	Transmembrane	7 – 27	21	Helical; Potential
	Topological domain	28 – 63	36	Cytoplasmic Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Topological domain	85 – 312	228	Extracellular Potential
Sites
	Active site	88	1	By similarity
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9R0 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 63602053A16A0D8B	FASTA	312	37,103
	10 20 30 40 50 60 MRNILTIFLL TSTFFTGILW IIDHILLIKN YFYNKKKTKN NNTILINKVI LENKKCFFRS 70 80 90 100 110 120 LSSLFPTFFI VFIIRSFIYE PFQIPSGSMM PTLLIGDFIL VKKFSYGIKE PITNKTIIKM 130 140 150 160 170 180 NLPQRGDIVV FKHPKNNIDY IKRVVGLPGD KIQYDINRKK IKICINYTNQ KNCENKLFIT 190 200 210 220 230 240 YSKPKLSNFF QKIYLLKSRT NEEEKVYNSI YFKKVEEKIN NLKHNILILD GINSKINDYY 250 260 270 280 290 300 QQKGMPKLIW IVPKNKYFMM GDNRDNSLDS RYWGFVPEEN LLGKATKIWM SFEKKENEWP 310 TGIRIKRIGN IY

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67809.1.
RefSeq	NP_660598.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9R0.
SMR	Q8K9R0. Positions 75-311.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg250.
Protein family/group databases
MEROPS	S26.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67809; AAM67809; BUsg_250.
GeneID	1005452.
KEGG	bas:BUsg250.
PATRIC	21247303. VBIBucAph100086_0261.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0681.
KO	K03100.
OMA	ESSHFGD.
ProtClustDB	CLSK315834.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-311-MONOMER.
Family and domain databases
Gene3D	2.10.109.10. 2 hits. 2.170.230.10. 1 hit.
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR019533. Peptidase_S26. IPR019766. Peptidase_S26A_all-beta_subdom. [Graphical view]
PANTHER	PTHR12383. PTHR12383. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. PF10502. Peptidase_S26. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_BUCAP

Accession

Primary (citable) accession number: Q8K9R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program