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Q8K9Q9 (LEPA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 4

Short name=EF-4
EC=3.6.5.n1
Alternative name(s):
Ribosomal back-translocase LepA
Gene names
Name:lepA
Ordered Locus Names:BUsg_251
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length601 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071

Catalytic activity

GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCell membrane
Membrane
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpositive regulation of translation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ribosome binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation elongation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 601601Elongation factor 4 HAMAP-Rule MF_00071
PRO_0000176247

Regions

Nucleotide binding18 – 236GTP By similarity
Nucleotide binding135 – 1384GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9Q9 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: AAEA8B0EF6091ECA

FASTA60167,748
        10         20         30         40         50         60 
MVKKMKNIRN FSIIAHIDHG KSTLSDRLIQ KCGGLSEREM SDQVLDSMDL EKERGITIKA 

        70         80         90        100        110        120 
QSVMIDYKDK NGNVFHLNFI DTPGHVNFSY EVSRSLAACE GALLVVDSSQ GVEAQTLANC 

       130        140        150        160        170        180 
ATALEMKLSI VPVLNKIDLP NSNPEKVARE IKEIIGISAL DAIRCSAKTG QGIEELIEQI 

       190        200        210        220        230        240 
IKKIPAPDGD EKKPLQALII DSWFDNYLGV VSLIRIKNGI ISKKDKIKVM STGKTYFVDH 

       250        260        270        280        290        300 
LGIFTPKKIN KNYLKCGEVG WIVCGIKNIS AAPVGDTLTN AKNPAMNMLT GFKKIKPQIY 

       310        320        330        340        350        360 
AGLFPVTSDQ YEIFRDALGK LSLNDSSLFY EPINSTALGF GFRCGFLGLL HMEIVQARLE 

       370        380        390        400        410        420 
REYSLNLIST APNVIYQIEF NNGEKIYLDT PSNFPVDNKI RKIKEPIVEC NILLPPKFLG 

       430        440        450        460        470        480 
PVMKLCIKKR GTEINMIYHE QQVSLKYHIP MNEVVLNFFD ELKSVSSGYA SLEYDFKYFK 

       490        500        510        520        530        540 
TVKMVRIDIL INSERVDALT VISYYKNAQN RAREIVNKMK ELIPRHQFDI NIQATINNSI 

       550        560        570        580        590        600 
VARSTIKQLR KNVLAKCYGG DISRKKKLLK KQKDGKKRMK KIGNVNMPKT AFLDILNIHK 


D 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67810.1.
RefSeqNP_660599.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9Q9.
SMRQ8K9Q9. Positions 5-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg251.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67810; AAM67810; BUsg_251.
GeneID1005453.
KEGGbas:BUsg251.
PATRIC21247305. VBIBucAph100086_0262.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0481.
KOK03596.
OMAKPMVFCG.
OrthoDBEOG6ZKXQ4.
ProtClustDBPRK05433.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-265-MONOMER.

Family and domain databases

Gene3D3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00071. LepA.
InterProIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPA_BUCAP
AccessionPrimary (citable) accession number: Q8K9Q9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names