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Q8K9P7

- TOP1_BUCAP

UniProt

Q8K9P7 - TOP1_BUCAP

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Protein
DNA topoisomerase 1
Gene
topA, BUsg_273
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalytic By similarity
Sitei33 – 331Interaction with DNA By similarity
Metal bindingi105 – 1051Magnesium 1; catalytic By similarity
Metal bindingi105 – 1051Magnesium 2 By similarity
Metal bindingi107 – 1071Magnesium 2 By similarity
Sitei162 – 1621Interaction with DNA By similarity
Sitei163 – 1631Interaction with DNA By similarity
Sitei166 – 1661Interaction with DNA By similarity
Sitei171 – 1711Interaction with DNA By similarity
Sitei178 – 1781Interaction with DNA By similarity
Active sitei313 – 3131O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei315 – 3151Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri589 – 62133C4-type 1UniRule annotation
Add
BLAST
Zinc fingeri649 – 67628C4-type 2UniRule annotation
Add
BLAST
Zinc fingeri699 – 72426C4-type 3UniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
  5. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-287-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:BUsg_273
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 853853DNA topoisomerase 1UniRule annotation
PRO_0000145145Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg273.

Structurei

3D structure databases

ProteinModelPortaliQ8K9P7.
SMRiQ8K9P7. Positions 3-562, 733-848.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 136134Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1916Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9P7-1 [UniParc]FASTAAdd to Basket

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MCKSLVIVES PVKAKTISKY LGKQYIVKSS VGHVRDLINN KSKNKKNIKK    50
SNIKHNEKTA LIQQMGINPY KNWKAEYHIL PGKEKIISEL KNIANKVHYI 100
YLATDLDREG EAIAWHLKEV IGGNSLKFRR VVFNEITKKS IEKAFQNIGK 150
INMNRVYSQQ ARRFMDRIVG YMISPLLWKK ISRGLSAGRV QSAAVRLITD 200
REYEIKNFIE REHWKISLSL LSKENKKVTM DITHYRNKTF LLNNKKEVNS 250
TIEKIKNLSF IITDRKDKIF KKKPPAPLIT STLQQASNID LGFSVKKTMF 300
LAQKLYEQGY ITYIRTDSYF LSNYAIEKVR SYIENFYGSD FLPKKSNIYS 350
NQKYSQEAHE AIRPSDVQVV NIDNCDPDAK KLYKLIWNYF IASQMKSERY 400
KSIKTTVMAD VFKLQSNTKI VLFSGWTKIL KKSNNVNFKF PVLDIGTTLI 450
LDKILPHQIF TKPPPRFSES SLVRELEKKG IGRPSTYATI ITKIKEKGYL 500
KIKKNKFYAA KIGEILITRL KKNFSDLVDY DFTARMEKNL DQVSDKLINW 550
KHLLNSFFDN FSQQLEQAKK PPEEGGMELN TTVPTEIDCS LCNKKMGIKT 600
AVTGVFLSCL GYNSEPNEKR CKNTINLISL NDLSTTKKEE KNFHLKNRCK 650
KCNLVMDVYL INENLKIFIC INNPSCNGYN LKKGIFKKSL NCSLKKIKCE 700
KCKNDMLFKT GRFGNFFMCI NDTCKNTRKI LPNGEISEPK FEPIPFPDIL 750
CEKSNTWFVL REGISGIFFA ANTFPKSRET RSPFVEELAR FEYLLPKKLH 800
YLASAPQRDN KGNKTIVCFN KLNKQQYIAS KKEGKFTGWA AFFIDRKWCI 850
VNK 853
Length:853
Mass (Da):98,816
Last modified:October 1, 2002 - v1
Checksum:i99B454AB78AC6D1B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67831.1.
RefSeqiNP_660620.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67831; AAM67831; BUsg_273.
GeneIDi1005477.
KEGGibas:BUsg273.
PATRICi21247351. VBIBucAph100086_0285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67831.1 .
RefSeqi NP_660620.1. NC_004061.1.

3D structure databases

ProteinModelPortali Q8K9P7.
SMRi Q8K9P7. Positions 3-562, 733-848.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg273.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67831 ; AAM67831 ; BUsg_273 .
GeneIDi 1005477.
KEGGi bas:BUsg273.
PATRICi 21247351. VBIBucAph100086_0285.

Phylogenomic databases

eggNOGi COG0551.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci BAPH198804:GHMG-287-MONOMER.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiTOP1_BUCAP
AccessioniPrimary (citable) accession number: Q8K9P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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