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Q8K9P7

- TOP1_BUCAP

UniProt

Q8K9P7 - TOP1_BUCAP

Protein

DNA topoisomerase 1

Gene

topA

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
    Sitei33 – 331Interaction with DNAUniRule annotation
    Metal bindingi105 – 1051Magnesium 1; catalyticUniRule annotation
    Metal bindingi105 – 1051Magnesium 2UniRule annotation
    Metal bindingi107 – 1071Magnesium 2UniRule annotation
    Sitei162 – 1621Interaction with DNAUniRule annotation
    Sitei163 – 1631Interaction with DNAUniRule annotation
    Sitei166 – 1661Interaction with DNAUniRule annotation
    Sitei171 – 1711Interaction with DNAUniRule annotation
    Sitei178 – 1781Interaction with DNAUniRule annotation
    Active sitei313 – 3131O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei315 – 3151Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri589 – 62133C4-type 1Add
    BLAST
    Zinc fingeri649 – 67628C4-type 2Add
    BLAST
    Zinc fingeri699 – 72426C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
    4. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-287-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:BUsg_273
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 853853DNA topoisomerase 1PRO_0000145145Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi198804.BUsg273.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K9P7.
    SMRiQ8K9P7. Positions 3-562, 733-848.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 136134ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni186 – 1916Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri589 – 62133C4-type 1Add
    BLAST
    Zinc fingeri649 – 67628C4-type 2Add
    BLAST
    Zinc fingeri699 – 72426C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K9P7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCKSLVIVES PVKAKTISKY LGKQYIVKSS VGHVRDLINN KSKNKKNIKK    50
    SNIKHNEKTA LIQQMGINPY KNWKAEYHIL PGKEKIISEL KNIANKVHYI 100
    YLATDLDREG EAIAWHLKEV IGGNSLKFRR VVFNEITKKS IEKAFQNIGK 150
    INMNRVYSQQ ARRFMDRIVG YMISPLLWKK ISRGLSAGRV QSAAVRLITD 200
    REYEIKNFIE REHWKISLSL LSKENKKVTM DITHYRNKTF LLNNKKEVNS 250
    TIEKIKNLSF IITDRKDKIF KKKPPAPLIT STLQQASNID LGFSVKKTMF 300
    LAQKLYEQGY ITYIRTDSYF LSNYAIEKVR SYIENFYGSD FLPKKSNIYS 350
    NQKYSQEAHE AIRPSDVQVV NIDNCDPDAK KLYKLIWNYF IASQMKSERY 400
    KSIKTTVMAD VFKLQSNTKI VLFSGWTKIL KKSNNVNFKF PVLDIGTTLI 450
    LDKILPHQIF TKPPPRFSES SLVRELEKKG IGRPSTYATI ITKIKEKGYL 500
    KIKKNKFYAA KIGEILITRL KKNFSDLVDY DFTARMEKNL DQVSDKLINW 550
    KHLLNSFFDN FSQQLEQAKK PPEEGGMELN TTVPTEIDCS LCNKKMGIKT 600
    AVTGVFLSCL GYNSEPNEKR CKNTINLISL NDLSTTKKEE KNFHLKNRCK 650
    KCNLVMDVYL INENLKIFIC INNPSCNGYN LKKGIFKKSL NCSLKKIKCE 700
    KCKNDMLFKT GRFGNFFMCI NDTCKNTRKI LPNGEISEPK FEPIPFPDIL 750
    CEKSNTWFVL REGISGIFFA ANTFPKSRET RSPFVEELAR FEYLLPKKLH 800
    YLASAPQRDN KGNKTIVCFN KLNKQQYIAS KKEGKFTGWA AFFIDRKWCI 850
    VNK 853
    Length:853
    Mass (Da):98,816
    Last modified:October 1, 2002 - v1
    Checksum:i99B454AB78AC6D1B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67831.1.
    RefSeqiNP_660620.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67831; AAM67831; BUsg_273.
    GeneIDi1005477.
    KEGGibas:BUsg273.
    PATRICi21247351. VBIBucAph100086_0285.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67831.1 .
    RefSeqi NP_660620.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali Q8K9P7.
    SMRi Q8K9P7. Positions 3-562, 733-848.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg273.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67831 ; AAM67831 ; BUsg_273 .
    GeneIDi 1005477.
    KEGGi bas:BUsg273.
    PATRICi 21247351. VBIBucAph100086_0285.

    Phylogenomic databases

    eggNOGi COG0551.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci BAPH198804:GHMG-287-MONOMER.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR013263. TopoI_Znr_bac.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
    PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiTOP1_BUCAP
    AccessioniPrimary (citable) accession number: Q8K9P7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3