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Q8K9P1 (BIOB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:BUsg_280
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342Biotin synthase HAMAP-Rule MF_01694
PRO_0000185548

Sites

Metal binding511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding951Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1861Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2581Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9P1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 640502D4AF1C8863

FASTA34239,089
        10         20         30         40         50         60 
MKRKWNLEDT KKLFSKPFFD LIFEAQKKHR EYFNPNRIQI STLLSIKTGA CPEDCKYCPQ 

        70         80         90        100        110        120 
SSRYKTGLKK EPLLEIEQIL KAAKKAKSSG STRFCMGAAW KNPKEKDMPY LEKIVKEVKK 

       130        140        150        160        170        180 
MGMETCMTLG TLNNSQAKKL AKAGLDFYNH NLDTSSNFYS SIITTRTYEE RLSTLKKVRN 

       190        200        210        220        230        240 
SGMKICSGGI IGLGEKKQDR MELLMELSNL SIQPESVPIN MLVKIPGTPM EKNENVDPFE 

       250        260        270        280        290        300 
FIRVIAATRI MMPKSYIRLS AGRENMSDET QAMCFMAGAN SIFYGCKLLT SSNPKEEKDQ 

       310        320        330        340 
KLFEKLDLFP DYKIQSITEE NKNNENLKIS DVNKAQYYNA AI 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67837.1.
RefSeqNP_660626.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9P1.
SMRQ8K9P1. Positions 4-310.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg280.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67837; AAM67837; BUsg_280.
GeneID1005484.
KEGGbas:BUsg280.
PATRIC21247367. VBIBucAph100086_0293.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2300099.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-294-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_BUCAP
AccessionPrimary (citable) accession number: Q8K9P1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names