ID   QUEF_BUCAP              Reviewed;         262 AA.
AC   Q8K9N6; O69229;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 49.
DE   RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
DE            EC=1.7.1.13;
DE   AltName: Full=7-cyano-7-carbaguanine reductase;
DE   AltName: Full=PreQ(0) reductase;
DE   AltName: Full=NADPH-dependent nitrile oxidoreductase;
GN   Name=queF; OrderedLocusNames=BUsg_288;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 15-262.
RX   MEDLINE=95170548; PubMed=7765846; DOI=10.1007/BF00296197;
RA   Kolibachuk D., Baumann P.;
RT   "Buchnera aphidicola (aphid-endosymbiont) glyceraldehyde-3-phosphate
RT   dehydrogenase: molecular cloning and sequence analysis.";
RL   Curr. Microbiol. 30:133-136(1995).
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-
CC       deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-
CC       cyano-7-carbaguanine + 2 NADPH.
CC   -!- PATHWAY: tRNA modification; queuosine-tRNA biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type
CC       2 subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AE013218; AAM67844.1; -; Genomic_DNA.
DR   EMBL; U11045; AAC05800.1; -; Genomic_DNA.
DR   RefSeq; NP_660633.1; -.
DR   GeneID; 1005492; -.
DR   GenomeReviews; AE013218_GR; BUsg_288.
DR   KEGG; bas:BUsg288; -.
DR   HOGENOM; Q8K9N6; -.
DR   OMA; Q8K9N6; EHNEFHE.
DR   BioCyc; BAPH198804:BUSG288-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003934; F:GTP cyclohydrolase I activity; IEA:InterPro.
DR   GO; GO:0046857; F:oxidoreductase activity, acting on other ni...; IEA:HAMAP.
DR   GO; GO:0033739; F:queuine synthase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:HAMAP.
DR   GO; GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_00817; atypical; 1.
DR   InterPro; IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
DR   InterPro; IPR001474; GTP_CycHdrlase_I.
DR   Pfam; PF01227; GTP_cyclohydroI; 1.
DR   PIRSF; PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.
DR   TIGRFAMs; TIGR03138; QueF; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; NADP; Oxidoreductase;
KW   Queuosine biosynthesis.
FT   CHAIN         1    262       NADPH-dependent 7-cyano-7-deazaguanine
FT                                reductase.
FT                                /FTId=PRO_0000163023.
FT   CONFLICT     28     28       L -> I (in Ref. 2; AAC05800).
SQ   SEQUENCE   262 AA;  30731 MW;  2A78FCFA2D946DCE CRC64;
     MSLKINNFNF LRPISRKKHR KKIKLNCLNL PFKGKDIWTL YELSWLNKNG LPQIAIAKIE
     IDVNSANIIE SKSFKIYINS FNQMKFNNNI DFINILTNDL TKCICGQISI KLFSLDAIKN
     ETITDFHGIC IDNQNIKIES YKYTPSFLMI NSERKIIKED LYTHLFKSNC PVTQQPDWAS
     IYIAYTGLSI NHASLLRYLI SFRSHNEFHE ECIERIFNDI NNICKPEELS VYARYTRRGG
     IDINPWRSNT NFSPFLTRLA RQ
//