Reviewed, UniProtKB/Swiss-Prot Q8K9N6 (QUEF_BUCAP)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	BUsg_288

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163023
Experimental info
	Sequence conflict	28	1	L → I in AAC05800. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8K9N6-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 2A78FCFA2D946DCE	FASTA	262	30,731
	10 20 30 40 50 60 MSLKINNFNF LRPISRKKHR KKIKLNCLNL PFKGKDIWTL YELSWLNKNG LPQIAIAKIE 70 80 90 100 110 120 IDVNSANIIE SKSFKIYINS FNQMKFNNNI DFINILTNDL TKCICGQISI KLFSLDAIKN 130 140 150 160 170 180 ETITDFHGIC IDNQNIKIES YKYTPSFLMI NSERKIIKED LYTHLFKSNC PVTQQPDWAS 190 200 210 220 230 240 IYIAYTGLSI NHASLLRYLI SFRSHNEFHE ECIERIFNDI NNICKPEELS VYARYTRRGG 250 260 IDINPWRSNT NFSPFLTRLA RQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Buchnera aphidicola (aphid-endosymbiont) glyceraldehyde-3-phosphate dehydrogenase: molecular cloning and sequence analysis." Kolibachuk D., Baumann P. Curr. Microbiol. 30:133-136(1995) [PubMed: 7765846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 15-262.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67844.1. U11045 Genomic DNA. Translation: AAC05800.1.
RefSeq	NP_660633.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005492.
GenomeReviews	Gene locus BUsg_288 in contig AE013218_GR.
KEGG	bas:BUsg288.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9N6.
OMA	EHNEFHE.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG288-MON.
Family and domain databases
HAMAP	MF_00817. Divergent sequence. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BUCAP

Accession

Primary (citable) accession number: Q8K9N6
Secondary accession number(s): O69229

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)