ID   K6PF_BUCAP              Reviewed;         320 AA.
AC   Q8K9N0;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 45.
DE   RecName: Full=6-phosphofructokinase;
DE            Short=Phosphofructokinase;
DE            EC=2.7.1.11;
DE   AltName: Full=Phosphohexokinase;
GN   Name=pfkA; OrderedLocusNames=BUsg_295;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + D-fructose 6-phosphate = ADP + D-
CC       fructose 1,6-bisphosphate.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 3/4.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the phosphofructokinase family.
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DR   EMBL; AE013218; AAM67850.1; -; Genomic_DNA.
DR   RefSeq; NP_660639.1; -.
DR   HSSP; P06998; 2PFK.
DR   SMR; Q8K9N0; 1-320.
DR   GeneID; 1005499; -.
DR   GenomeReviews; AE013218_GR; BUsg_295.
DR   KEGG; bas:BUsg295; -.
DR   HOGENOM; Q8K9N0; -.
DR   OMA; Q8K9N0; DTCLNTV.
DR   BioCyc; BAPH198804:BUSG295-MON; -.
DR   GO; GO:0005945; C:6-phosphofructokinase complex; IEA:InterPro.
DR   GO; GO:0003872; F:6-phosphofructokinase activity; IEA:HAMAP.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006002; P:fructose 6-phosphate metabolic process; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:HAMAP.
DR   HAMAP; MF_00339; -; 1.
DR   InterPro; IPR012003; ATP_PFK_prok.
DR   InterPro; IPR012828; PFKA_ATP.
DR   InterPro; IPR000023; Phosphofructokinase.
DR   InterPro; IPR015912; Phosphofructokinase_CS.
DR   Pfam; PF00365; PFK; 1.
DR   PIRSF; PIRSF000532; ATP_PFK_prok; 1.
DR   PRINTS; PR00476; PHFRCTKINASE.
DR   ProDom; PD000707; Ppfruckinase; 1.
DR   TIGRFAMs; TIGR02482; PFKA_ATP; 1.
DR   PROSITE; PS00433; PHOSPHOFRUCTOKINASE; 1.
PE   3: Inferred from homology;
KW   Allosteric enzyme; ATP-binding; Complete proteome; Cytoplasm;
KW   Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding;
KW   Transferase.
FT   CHAIN         1    320       6-phosphofructokinase.
FT                                /FTId=PRO_0000111941.
FT   NP_BIND      22     26       ATP (By similarity).
FT   NP_BIND     155    159       ATP (By similarity).
FT   NP_BIND     172    188       ATP (By similarity).
FT   ACT_SITE    128    128       Proton acceptor (By similarity).
FT   METAL       186    186       Magnesium; via carbonyl oxygen (By
FT                                similarity).
FT   METAL       188    188       Magnesium (By similarity).
FT   BINDING     163    163       Substrate (By similarity).
FT   BINDING     244    244       Substrate (By similarity).
FT   BINDING     250    250       Substrate (By similarity).
FT   BINDING     253    253       Substrate (By similarity).
SQ   SEQUENCE   320 AA;  35239 MW;  FA57FB8507548ED1 CRC64;
     MIKKIGVLTS GGDAPGMNAA IRGVVRTALS EKLEVFGIYD GYLGLYENRM TLLDRYSVSD
     MINRGGTFLG SARFPGFYKN EIRTIAVNNL NKRNIDALVV IGGDGSYIGA QKLTKMGIPC
     ISIPGTIDND VAGTDYTIGY FTALETVVEA IDRLRDTSSS HQRISIVEVM GRYCGDLTLA
     AAIAGGCEFI VLPEIHYKKE ELVSEIQAGI EKGKKHAIVA ITEYICDVEK LAKYIEKKTN
     RETRATILGH IQRGGAPVVY DRILASRMGA YAVELLIEGY KGKCVGIQNE KMVFNDITDA
     LKNMKRVFKK DWLITAKKLY
//