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Q8K9M3 (KPYK_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pykA
Ordered Locus Names:BUsg_311
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Enzyme regulation

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481Pyruvate kinase
PRO_0000112059

Sites

Metal binding381Potassium By similarity
Metal binding401Potassium By similarity
Metal binding701Potassium By similarity
Metal binding2251Magnesium By similarity
Metal binding2521Magnesium By similarity
Binding site361Substrate By similarity
Binding site2511Substrate; via amide nitrogen By similarity
Binding site2521Substrate; via amide nitrogen By similarity
Binding site2841Substrate By similarity
Site2231Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9M3 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 8A11ECA79EAB8A3B

FASTA48152,632
        10         20         30         40         50         60 
MLNRVRRTKI VATLGPSTDK ANNLEKIILS GANVLRLNFS HGSSEEHKKR AKKAKEIMFK 

        70         80         90        100        110        120 
LNCHIALLGD LQGPKIRISK FKKNSIFLKV NDVFILDANL NEKNGNNERV GIDYKKLPND 

       130        140        150        160        170        180 
LNVNDILLLD DGRIQLKVIK IEKCAIFTKV IIGGILSNNK GINKLGGGLS ADALTEKDKK 

       190        200        210        220        230        240 
DIILASEIDV DYLAVSFPRC ADDLIKAREL LKKSGSNAQI IAKIERAEAV FDQNAIENII 

       250        260        270        280        290        300 
LSSDAIMIAR GDLGVEIGDA ELVGIQKKLI RTARKLNKVA ITATQMMESM ILNPLPTRAE 

       310        320        330        340        350        360 
VMDVANAVLD GSDAVMLSAE TASGKYPSET VISMAKVCKG AEKVPSINVS RHRLNVEFQS 

       370        380        390        400        410        420 
IEEAIAMSSM YAANHLKGIT AIITMTESGK TALMTSRITS GLPIFALSKH KKTLNLTALY 

       430        440        450        460        470        480 
RGVIPIYFDS DKDGVEAANE AIILLCKKKF LFNNDLVIVT QGDIMGKIGK TNTSRILRVT 


Y 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67865.1.
RefSeqNP_660654.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9M3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg311.

Proteomic databases

PRIDEQ8K9M3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67865; AAM67865; BUsg_311.
GeneID1005515.
KEGGbas:BUsg311.
PATRIC21247429. VBIBucAph100086_0324.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0469.
KOK00873.
OMATPVYFDS.
OrthoDBEOG6GBMB0.
ProtClustDBCLSK315882.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-325-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_BUCAP
AccessionPrimary (citable) accession number: Q8K9M3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: November 13, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names