Reviewed, UniProtKB/Swiss-Prot Q8K9L2 (PYRC_BUCAP)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	BUsg_322

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6. HAMAP MF_00219
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Dihydroorotase HAMAP MF_00219		PRO_0000147204
Sites
	Metal binding	17	1	Zinc 1 By similarity
	Metal binding	19	1	Zinc 1 By similarity
	Metal binding	103	1	Zinc 1; via carbamate group By similarity
	Metal binding	103	1	Zinc 2; via carbamate group By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	178	1	Zinc 2 By similarity
	Metal binding	252	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	103	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9L2-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 03D6ADFD7BCE0BC5	FASTA	349	40,557
	10 20 30 40 50 60 MSKFLKKIKI IKPDDWHVHL RDNEILKKVS QYTGTFYKRA IIMPNLEEPI TNCFRSISYR 70 80 90 100 110 120 RRILNSMTPN TAFQPLMICY LTEKTSPEEL QQGFFKKIFV GAKLYPHCST TNSKYGIKNI 130 140 150 160 170 180 NNIYHLFNIM EKIKMPLLIH GEENNLNIDI YDREAKFIEN TLKPLRKKFP ELKIILEHIT 190 200 210 220 230 240 TEEAISYIEE CNSSYLAGTI TPHHLMLNRN NMFIDGIQPH LYCLPLLKRK KHQIALRNVI 250 260 270 280 290 300 SSGSKNFFLG SDTAPHFHKN KINTFGCAGI FNAPSSLLCY VSVFEEMNAL QHFQSFCSEN 310 320 330 340 GPNFYNMPIN KETITLVKKP HKILEKINIG KNIIVPFLAG KRLDWSIEK

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67876.1.
RefSeq	NP_660665.1.
3D structure databases
HSSP	HSSP built from PDB template 1J79 based on UniProtKB P05020.
ModBase	Search...
Genome annotation databases
GeneID	1005527.
GenomeReviews	Gene locus BUsg_322 in contig AE013218_GR.
KEGG	bas:BUsg322.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9L2.
OMA	Q8K9L2. IMPNLVP.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG322-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_BUCAP

Accession

Primary (citable) accession number: Q8K9L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)