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Q8K9J2 (HOLB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase III subunit delta'

EC=2.7.7.7
Gene names
Name:holB
Ordered Locus Names:BUsg_342
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Ontologies

Keywords
   Biological processDNA replication
   Molecular functionDNA-directed DNA polymerase
Nucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentDNA polymerase III complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed DNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328DNA polymerase III subunit delta'
PRO_0000105511

Sequences

Sequence LengthMass (Da)Tools
Q8K9J2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 160BA0FE21BAD08D

FASTA32838,927
        10         20         30         40         50         60 
MKWYPWLIKP YKKIIRLHER KKAHHAILIK TQKGMGVFKL VWFISKWLLC LKPKGTNFCD 

        70         80         90        100        110        120 
NCHGCKLMSA NNHPDWHTII NEKNDVFDVD SIRVINEKIF KRAQQGNNKI IFLPNVHKLT 

       130        140        150        160        170        180 
ESAVNALLKI LEEPPEKNWF FLIDYNYLKL HSTLKSRCFL YRLPLPLEKE SLNWLKNNNK 

       190        200        210        220        230        240 
KDNISNLTSL RINQDSPISA KNFIESDLWD ERKNLYKSLS HSIKDKNLLK ILPVLCKKNT 

       250        260        270        280        290        300 
IMKIDWICLL LFDAVKTNFN ERKKLINCDQ IKLINFFSKK YNNILLNKSI RKWTKCRYIL 

       310        320 
SSVSSINSEL LLLEQLLLWE KILCFITP 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67896.1.
RefSeqNP_660685.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9J2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg342.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67896; AAM67896; BUsg_342.
GeneID1005577.
KEGGbas:BUsg342.
PATRIC21247499. VBIBucAph100086_0356.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0470.
KOK02341.
OMACHSCHLM.
OrthoDBEOG6W9X5M.
ProtClustDBCLSK493055.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-359-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR008921. DNA_pol3_clamp-load_cplx_C.
IPR015199. DNA_pol_III_delta_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF09115. DNApol3-delta_C. 1 hit.
[Graphical view]
SUPFAMSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHOLB_BUCAP
AccessionPrimary (citable) accession number: Q8K9J2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names