Reviewed, UniProtKB/Swiss-Prot Q8K9J0 (PTGCB_BUCAP)

Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICB component
Alternative name(s):
    EIICB-Glc
      Short name=EII-Glc
Including the following 2 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component

Gene names

Name:	ptsG
Ordered Locus Names:	BUsg_344

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose transport Inferred from electronic annotation. Source: InterPro phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	PTS system glucose-specific EIICB component		PRO_0000186526
Regions
	Transmembrane	20 – 40	21	Potential
	Transmembrane	51 – 71	21	Potential
	Transmembrane	76 – 96	21	Potential
	Transmembrane	112 – 132	21	Potential
	Transmembrane	152 – 172	21	Potential
	Transmembrane	250 – 270	21	Potential
	Transmembrane	280 – 300	21	Potential
	Transmembrane	304 – 324	21	Potential
	Transmembrane	354 – 374	21	Potential
	Domain	1 – 388	388	PTS EIIC type-1
	Domain	399 – 477	79	PTS EIIB type-1
Sites
	Active site	421	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9J0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 84CA2ABDFEA4CFA2	FASTA	477	51,941
	10 20 30 40 50 60 MFKNVFSSLQ KVGKSLMLPV SVLPIAGILL GIGSAHFTLI PEIISQIMAQ TGGSIFSNMP 70 80 90 100 110 120 LIFAIGVALG FSNNDGVAAL AAVVAYSILI QTLSAVELNI LNTDANTIKN KNFSDIGILG 130 140 150 160 170 180 GIIAGAISAY MFNKFYKIQL PEYLGFFAGK RFVPIISGLF AIFVGLILSL IWPSIGNKIQ 190 200 210 220 230 240 IFSEWAAYQN PIIAFSLYGL VERALVPFGL HHIWNVPFQM QIGEYKNSIG QIFHGDIARY 250 260 270 280 290 300 MAGDTTAGNL SGGFIFKMYG LPGAALAIWH TAKKENRKKI GSIMISAALT AFLTGITEPI 310 320 330 340 350 360 EFSFILVAPI LYIIHAILAG LSFPLCIFLN MRAGTSFSHG FIDFIVLSGH SHKIFLFPIV 370 380 390 400 410 420 GICYGLLYYS IFYFLITTFN LKTPGREENK NTVFFRNNIE IAPYIVEALG GKNNIKNLDA 430 440 450 460 470 CITRLRITVS EISKVNEENL KNLGAAGVVI SGSGVQAVFG TRSENIKTAI DEYINNI

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67898.1.
RefSeq	NP_660687.1.
3D structure databases
HSSP	HSSP built from PDB template 1IBA based on UniProtKB P05053.
ModBase	Search...
Genome annotation databases
GeneID	1005590.
GenomeReviews	Gene locus BUsg_344 in contig AE013218_GR.
KEGG	bas:BUsg344.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9J0.
OMA	HILITLA.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG344-MON.
Family and domain databases
InterPro	IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001996. PTS_EIIB. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTGCB_BUCAP

Accession

Primary (citable) accession number: Q8K9J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)