Reviewed, UniProtKB/Swiss-Prot Q8K9I7 (SYN_BUCAP)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	BUsg_348

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176398

Sequences

	Sequence		Length	Mass (Da)
	Q8K9I7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 9D8CE81FE0EF218D	FASTA	466	53,868
	10 20 30 40 50 60 MSRISISEIY KDDIIVNTPI TIFGWVRSRR SSKSGFSFIT VYDGSCLNSV QVVADKTLSN 70 80 90 100 110 120 YYKDILHLTI GCSVTITGIL ILSIGDKQKY EIKATKFQVL GWIKNPDTYP ISAKKHSLEY 130 140 150 160 170 180 LREVAHLRSR TNLIGVIVRI RHHIFQSLHK FLNKQGYYWI PTPIITGLNT EGTGEMFRVS 190 200 210 220 230 240 TMDMRNIPKK INGSVDFKKD FFGKESFLTV SGQLNLEAYA CSLSKVYTFG PTFRAENSNT 250 260 270 280 290 300 SRHLAEFWML EIESSFCNLD EILIFSEDML KYICKSLLKY CINDIKFLKN YIDNDIINRL 310 320 330 340 350 360 KKFLSVNFIR INYKDAIDIL LNSKKKFDNV VSFGVDLNAE HERFLVEKHF KAPVVIINYP 370 380 390 400 410 420 KELKAFYMRL NDDKKTVAAM DLLVPGIGEL IGGSQREERI SVLDLRLSEL GLRKEDYWWY 430 440 450 460 RDLRRYGTVH HSGFGMGFER LISYITGISN IRDIIPFPRT VKNADF

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67901.1.
RefSeq	NP_660690.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005808.
GenomeReviews	Gene locus BUsg_348 in contig AE013218_GR.
KEGG	bas:BUsg348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9I7.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG348-MON.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_BUCAP

Accession

Primary (citable) accession number: Q8K9I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)