Reviewed, UniProtKB/Swiss-Prot Q8K9I5 (PYRD_BUCAP)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BUsg_350

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_0000148427
Sites
	Active site	175	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9I5-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 19B8F807BF49DE83	FASTA	336	38,101
	10 20 30 40 50 60 MFYYLIRKLL FLIDPEKAHT LVLMYLNSKK IQILRKIFIK PIPLKQIKCM GLTFNNKIGL 70 80 90 100 110 120 AAGMDKNGDY IDSLSKIGFG FIEVGTVTPL PQYGNPKPRI FRVPSIEGII NRMGFNNLGI 130 140 150 160 170 180 DYLVNNVKKS KFKGIIGINI GKNKDTKIED AIKDYLICIE KIYFYASYIA INISSPNTIN 190 200 210 220 230 240 LRKLQYGILF ENLLRDIKKK QSEMHSKYSK YVPIVIKISP DLSKKELIHI SNKLIHYKID 250 260 270 280 290 300 GVIATNTTLD HSSIPKIKNN KEEGGLSGLP LQKKSNDVIS ILYKNLKRKI PIIGVGGINS 310 320 330 INSAREKIMC GANLIQVYSG LIYHGPNFIR EIIRNL

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67903.1.
RefSeq	NP_660692.1.
3D structure databases
HSSP	HSSP built from PDB template 1F76 based on UniProtKB P05021.
ModBase	Search...
Genome annotation databases
GeneID	1005806.
GenomeReviews	Gene locus BUsg_350 in contig AE013218_GR.
KEGG	bas:BUsg350.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9I5.
OMA	Q8K9I5. NAEQQGG.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG350-MON.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BUCAP

Accession

Primary (citable) accession number: Q8K9I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)