Q8K9I0 (AMPA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BUsg_355

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165731
Sites
	Active site	282	1	Potential
	Active site	356	1	Potential
	Metal binding	270	1	Manganese 2 By similarity
	Metal binding	275	1	Manganese 1 By similarity
	Metal binding	275	1	Manganese 2 By similarity
	Metal binding	293	1	Manganese 2 By similarity
	Metal binding	352	1	Manganese 1 By similarity
	Metal binding	354	1	Manganese 1 By similarity
	Metal binding	354	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9I0 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 3A04E5BCF255713D	FASTA	502	56,041
	10 20 30 40 50 60 MKFFIKNVLL EEEKSDCIVI GVFEFCEFKY SNNYLNKSIC SYINNFIKKG DMQGKVGETL 70 80 90 100 110 120 LLYDVPNVVS KRILLVGCGK KNRLNRYYLD KIINKSMKIL NKFSIKNIIF SLTEINIENY 130 140 150 160 170 180 DIYWSIRTIV NSINKYLYKN FKINAFLKKE VYLNSISFHI IEKKDFHIAN NSLKHALAIN 190 200 210 220 230 240 SGITAAKNLA DLPPNICNPL YLSLQAQKLS EKYKDKIHVT SIDIKEMNNL GMNAYVAVGK 250 260 270 280 290 300 GSKNKPYMSV IKYSGINNTE QEKIIVLIGK GLTFDSGGIS IKPSNNMNEM KYDMCGAAAV 310 320 330 340 350 360 YGTLIAAAKL NLPLTIIGIL AGCENMPGGN AFRPGDIITT MSGKTVEILN TDAEGRLVLC 370 380 390 400 410 420 DVLTYVQRFS PNIVIDIATL TGACVVALGN HFSGLFSNDD QLAYALEKSS RQTNDKIWRL 430 440 450 460 470 480 PLSLEYEKEL HSNFADLSNV GRGKAGAITA SCFLAQFSKK YTWAHLDIAG TAWKSGKNHG 490 500 ATGRPVELLS QFLLNESNFL KF

References

[1]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67908.1.
RefSeq	NP_660697.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9I0.
SMR	Q8K9I0. Positions 1-501.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000382; EBBUCP00000000382; EBBUCG00000000382.
GeneID	1005800.
GenomeReviews	Gene locus BUsg_355 in contig AE013218_GR.
KEGG	bas:BUsg355.
PATRIC	21247525. VBIBucAph100086_0369.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008030.
HOGENOM	HBG742580.
OMA	MPANICT.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG355-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BUCAP

Accession

Primary (citable) accession number: Q8K9I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program