ID   PNP_BUCAP               Reviewed;         707 AA.
AC   Q8K9H5;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 50.
DE   RecName: Full=Polyribonucleotide nucleotidyltransferase;
DE            EC=2.7.7.8;
DE   AltName: Full=Polynucleotide phosphorylase;
DE            Short=PNPase;
GN   Name=pnp; OrderedLocusNames=BUsg_361;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Involved in mRNA degradation. Hydrolyzes single-stranded
CC       polyribonucleotides processively in the 3'- to 5'-direction (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: RNA(n+1) + phosphate = RNA(n) + a nucleoside
CC       diphosphate.
CC   -!- SUBUNIT: Homotrimer. Organized into a structure (processome or RNA
CC       degradosome) containing a number of RNA-processing enzymes (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the polyribonucleotide
CC       nucleotidyltransferase family.
CC   -!- SIMILARITY: Contains 1 KH domain.
CC   -!- SIMILARITY: Contains 1 S1 motif domain.
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DR   EMBL; AE013218; AAM67914.1; -; Genomic_DNA.
DR   RefSeq; NP_660703.1; -.
DR   HSSP; P05055; 1SRO.
DR   SMR; Q8K9H5; 617-691.
DR   GeneID; 1005794; -.
DR   GenomeReviews; AE013218_GR; BUsg_361.
DR   KEGG; bas:BUsg361; -.
DR   HOGENOM; Q8K9H5; -.
DR   OMA; Q8K9H5; GHGNLAK.
DR   BioCyc; BAPH198804:BUSG361-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000175; F:3'-5'-exoribonuclease activity; IEA:InterPro.
DR   GO; GO:0004654; F:polyribonucleotide nucleotidyltransferase a...; IEA:HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:HAMAP.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   HAMAP; MF_01595; -; 1.
DR   InterPro; IPR001247; ExoRNase_PH_dom1.
DR   InterPro; IPR015847; ExoRNase_PH_dom2.
DR   InterPro; IPR004087; KH.
DR   InterPro; IPR004088; KH_type_1.
DR   InterPro; IPR018111; KH_type_1_subgr.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR012162; PNPase.
DR   InterPro; IPR015848; PNPase_PH_RNA-bd_bac/org-type.
DR   InterPro; IPR003029; Rbsml_prot_S1_RNA-bd_dom.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Gene3D; G3DSA:1.10.10.400; PNPase_PH_RNA-bd_bac/org-type; 1.
DR   PANTHER; PTHR11252; PNPase; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF03726; PNPase; 1.
DR   Pfam; PF01138; RNase_PH; 2.
DR   Pfam; PF03725; RNase_PH_C; 2.
DR   Pfam; PF00575; S1; 1.
DR   PIRSF; PIRSF005499; PNPase; 1.
DR   SMART; SM00322; KH; 1.
DR   TIGRFAMs; TIGR03591; Polynuc_phos; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Nucleotidyltransferase; RNA-binding;
KW   Transferase.
FT   CHAIN         1    707       Polyribonucleotide
FT                                nucleotidyltransferase.
FT                                /FTId=PRO_0000197911.
FT   DOMAIN      553    612       KH.
FT   DOMAIN      622    690       S1 motif.
SQ   SEQUENCE   707 AA;  78389 MW;  9D5367E96E91520A CRC64;
     MLNPIVRKFQ YGQHTITLET GIMARQATAA VMASMDDTAV FVTVVGEKTT NSSQKFFPLT
     VNYQERTYAV GRIPGGFFRR EGRPSENEIL TARLIDRPIR PLFPKGFCNE IQIIATVVSV
     NPQINPDIIS IIGASAALSL SGIPFYGPIG AARVGFVNNQ YVLNPTIDDM KSSFLDLVVS
     GTQNAVLMVE AESKVLSEDK ILGAIMFGHQ QQQVVINNIR SLSNEASKLP WIISYPEINT
     ELELKITKLA EKDISNAYLI FNKQERYEKL NFLKEEIIKL FFSENSNIDI SEIEDIFEKI
     EKNIVRKRIL NNENRIDGRE KDMIRALDIR TGVLPRTHGS SLFTRGETQS LVSVTLGTSR
     DAQNLDELLG DKTDNFLFHY NFPPYSVGEI GIVGSPKRRE IGHGRLAKRS LLAVMPKLDD
     FPYTIRIVSE ITESNGSSSM ASVCGASLAL MDAGVPIKSA VAGISMGLVK EGDKYVLLSD
     ILGDEDHLGD MDFKVSGTEE GITALQMDIK IEGITNEIMR IALNKAKSAR LHILNVMKQA
     LSKPRNEISE FAPRIHKIKI NPEKIKDVIG KGGSVIRMLT EETGTIIEIE DDGTIKISAT
     IGEKAKNAIR RIEEITAEIE VGRIYSGKVT RIVDFGAFIS IGIGKEGLVH ISQISNKRVE
     KVSDHLTVDQ IISVKVLEID RQGRLRLSIK EVENSIISNK SINNIYI
//