Reviewed, UniProtKB/Swiss-Prot Q8K9H5 (PNP_BUCAP)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	BUsg_361

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000197911
Regions
	Domain	553 – 612	60	KH
	Domain	622 – 690	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q8K9H5-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 9D5367E96E91520A	FASTA	707	78,389
	10 20 30 40 50 60 MLNPIVRKFQ YGQHTITLET GIMARQATAA VMASMDDTAV FVTVVGEKTT NSSQKFFPLT 70 80 90 100 110 120 VNYQERTYAV GRIPGGFFRR EGRPSENEIL TARLIDRPIR PLFPKGFCNE IQIIATVVSV 130 140 150 160 170 180 NPQINPDIIS IIGASAALSL SGIPFYGPIG AARVGFVNNQ YVLNPTIDDM KSSFLDLVVS 190 200 210 220 230 240 GTQNAVLMVE AESKVLSEDK ILGAIMFGHQ QQQVVINNIR SLSNEASKLP WIISYPEINT 250 260 270 280 290 300 ELELKITKLA EKDISNAYLI FNKQERYEKL NFLKEEIIKL FFSENSNIDI SEIEDIFEKI 310 320 330 340 350 360 EKNIVRKRIL NNENRIDGRE KDMIRALDIR TGVLPRTHGS SLFTRGETQS LVSVTLGTSR 370 380 390 400 410 420 DAQNLDELLG DKTDNFLFHY NFPPYSVGEI GIVGSPKRRE IGHGRLAKRS LLAVMPKLDD 430 440 450 460 470 480 FPYTIRIVSE ITESNGSSSM ASVCGASLAL MDAGVPIKSA VAGISMGLVK EGDKYVLLSD 490 500 510 520 530 540 ILGDEDHLGD MDFKVSGTEE GITALQMDIK IEGITNEIMR IALNKAKSAR LHILNVMKQA 550 560 570 580 590 600 LSKPRNEISE FAPRIHKIKI NPEKIKDVIG KGGSVIRMLT EETGTIIEIE DDGTIKISAT 610 620 630 640 650 660 IGEKAKNAIR RIEEITAEIE VGRIYSGKVT RIVDFGAFIS IGIGKEGLVH ISQISNKRVE 670 680 690 700 KVSDHLTVDQ IISVKVLEID RQGRLRLSIK EVENSIISNK SINNIYI

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67914.1.
RefSeq	NP_660703.1.
3D structure databases
HSSP	HSSP built from PDB template 1SRO based on UniProtKB P05055.
SMR	Q8K9H5. Positions 617-691.
ModBase	Search...
Genome annotation databases
GeneID	1005794.
GenomeReviews	Gene locus BUsg_361 in contig AE013218_GR.
KEGG	bas:BUsg361.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9H5.
OMA	Q8K9H5. GHGNLAK.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG361-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_BUCAP

Accession

Primary (citable) accession number: Q8K9H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)