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Q8K9F7

- SRP54_BUCAP

UniProt

Q8K9F7 - SRP54_BUCAP

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Protein

Signal recognition particle protein

Gene

ffh

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 1148GTPUniRule annotation
Nucleotide bindingi190 – 1945GTPUniRule annotation
Nucleotide bindingi248 – 2514GTPUniRule annotation

GO - Molecular functioni

  1. 7S RNA binding Source: InterPro
  2. GTPase activity Source: UniProtKB-HAMAP
  3. GTP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. SRP-dependent cotranslational protein targeting to membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-398-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle proteinUniRule annotation
Alternative name(s):
Fifty-four homologUniRule annotation
Gene namesi
Name:ffhUniRule annotation
Ordered Locus Names:BUsg_380
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation
Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

GO - Cellular componenti

  1. signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Signal recognition particle proteinPRO_0000101151Add
BLAST

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg380.

Structurei

3D structure databases

ProteinModelPortaliQ8K9F7.
SMRiQ8K9F7. Positions 366-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0541.
KOiK03106.
OMAiDPKSQFI.
OrthoDBiEOG62K1ZH.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERiPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00959. ffh. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9F7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNNLTERLS QSLRKIINKG RLTEENIKDT IREVRKALLE ADVTLSVIKK
60 70 80 90 100
FIQNVSKKAI GHEINKSLTP GQEFIKIVKN ELILSMGEKN HDLNLSIQPP
110 120 130 140 150
AIILVVGLQG VGKTTTLVKL AKWIKEKYKK KILTVSTDIY RAAAIKQLQI
160 170 180 190 200
LSDQVKIDFY LSDTTQTPIN ITKEAIEHAK LKLYDLLLID TAGRLHINTE
210 220 230 240 250
MMNEINTIQN ISKPIETLLI VDSMMGQDAI NIAKKFSASL SISGIVITKT
260 270 280 290 300
DSDARSGVAL SIRHITGKPI KFIGTGEKLH QLEPFHPERI ADRILGMNQV
310 320 330 340 350
ISLIKDIEEK VNQSQVKNLT KKFRKGDDFN LNDFLIQLKE MKNMGNLNYF
360 370 380 390 400
IEKFSKNKIL SNNPLLGENK NTLNRIEAII YSMTHKERMH PIIIKGSRKR
410 420 430 440 450
RIALGSGTKI QDVNKLLKNF DNIKKIMKKI KKGGIGKMIR NISNILPKNF
Length:450
Mass (Da):51,017
Last modified:October 1, 2002 - v1
Checksum:i4A96BAFB8787D685
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67932.1.
RefSeqiNP_660721.1. NC_004061.1.
WP_011053899.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67932; AAM67932; BUsg_380.
GeneIDi1005777.
KEGGibas:BUsg380.
PATRICi21247578. VBIBucAph100086_0394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67932.1 .
RefSeqi NP_660721.1. NC_004061.1.
WP_011053899.1. NC_004061.1.

3D structure databases

ProteinModelPortali Q8K9F7.
SMRi Q8K9F7. Positions 366-431.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg380.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67932 ; AAM67932 ; BUsg_380 .
GeneIDi 1005777.
KEGGi bas:BUsg380.
PATRICi 21247578. VBIBucAph100086_0394.

Phylogenomic databases

eggNOGi COG0541.
KOi K03106.
OMAi DPKSQFI.
OrthoDBi EOG62K1ZH.

Enzyme and pathway databases

BioCyci BAPH198804:GHMG-398-MONOMER.

Family and domain databases

Gene3Di 1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00306. SRP54.
InterProi IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view ]
PANTHERi PTHR11564:SF7. PTHR11564:SF7. 1 hit.
Pfami PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00959. ffh. 1 hit.
PROSITEi PS00300. SRP54. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiSRP54_BUCAP
AccessioniPrimary (citable) accession number: Q8K9F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3