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Protein

Signal recognition particle protein

Gene

ffh

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 114GTPUniRule annotation8
Nucleotide bindingi190 – 194GTPUniRule annotation5
Nucleotide bindingi248 – 251GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-399-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle proteinUniRule annotation
Alternative name(s):
Fifty-four homologUniRule annotation
Gene namesi
Name:ffhUniRule annotation
Ordered Locus Names:BUsg_380
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Note: The SRP-RNC complex is targeted to the cytoplasmic membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001011511 – 450Signal recognition particle proteinAdd BLAST450

Proteomic databases

PRIDEiQ8K9F7

Interactioni

Subunit structurei

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg380

Structurei

3D structure databases

ProteinModelPortaliQ8K9F7
SMRiQ8K9F7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.UniRule annotation

Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CB9 Bacteria
COG0541 LUCA
KOiK03106
OMAiDTAGRHK
OrthoDBiPOG091H02J9

Family and domain databases

Gene3Di1.10.260.30, 1 hit
HAMAPiMF_00306 SRP54, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR036225 SRP/SRP_N
IPR022941 SRP54
IPR000897 SRP54_GTPase_dom
IPR004780 SRP_Ffh
PANTHERiPTHR11564 PTHR11564, 1 hit
PTHR11564:SF7 PTHR11564:SF7, 1 hit
PfamiView protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47364 SSF47364, 1 hit
SSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00959 ffh, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K9F7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNNLTERLS QSLRKIINKG RLTEENIKDT IREVRKALLE ADVTLSVIKK
60 70 80 90 100
FIQNVSKKAI GHEINKSLTP GQEFIKIVKN ELILSMGEKN HDLNLSIQPP
110 120 130 140 150
AIILVVGLQG VGKTTTLVKL AKWIKEKYKK KILTVSTDIY RAAAIKQLQI
160 170 180 190 200
LSDQVKIDFY LSDTTQTPIN ITKEAIEHAK LKLYDLLLID TAGRLHINTE
210 220 230 240 250
MMNEINTIQN ISKPIETLLI VDSMMGQDAI NIAKKFSASL SISGIVITKT
260 270 280 290 300
DSDARSGVAL SIRHITGKPI KFIGTGEKLH QLEPFHPERI ADRILGMNQV
310 320 330 340 350
ISLIKDIEEK VNQSQVKNLT KKFRKGDDFN LNDFLIQLKE MKNMGNLNYF
360 370 380 390 400
IEKFSKNKIL SNNPLLGENK NTLNRIEAII YSMTHKERMH PIIIKGSRKR
410 420 430 440 450
RIALGSGTKI QDVNKLLKNF DNIKKIMKKI KKGGIGKMIR NISNILPKNF
Length:450
Mass (Da):51,017
Last modified:October 1, 2002 - v1
Checksum:i4A96BAFB8787D685
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA Translation: AAM67932.1
RefSeqiWP_011053899.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM67932; AAM67932; BUsg_380
KEGGibas:BUsg_380

Similar proteinsi

Entry informationi

Entry nameiSRP54_BUCAP
AccessioniPrimary (citable) accession number: Q8K9F7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health