Q8K9F7 (SRP54_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle protein

Alternative name(s):
Fifty-four homolog

Gene names

Name:	ffh
Ordered Locus Names:	BUsg_380

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306
Subunit structure	Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity.
Subcellular location	Cytoplasm By similarity. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity.
Domain	Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306
Sequence similarities	Belongs to the GTP-binding SRP family. SRP54 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Signal recognition particle
Ligand	GTP-binding Nucleotide-binding RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC SRP-dependent cotranslational protein targeting to membrane Inferred from electronic annotation. Source: InterPro
Cellular_component	signal recognition particle, endoplasmic reticulum targeting Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	7S RNA binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Signal recognition particle protein HAMAP-Rule MF_00306		PRO_0000101151
Regions
	Nucleotide binding	107 – 114	8	GTP By similarity
	Nucleotide binding	190 – 194	5	GTP By similarity
	Nucleotide binding	248 – 251	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9F7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 4A96BAFB8787D685	FASTA	450	51,017
	10 20 30 40 50 60 MFNNLTERLS QSLRKIINKG RLTEENIKDT IREVRKALLE ADVTLSVIKK FIQNVSKKAI 70 80 90 100 110 120 GHEINKSLTP GQEFIKIVKN ELILSMGEKN HDLNLSIQPP AIILVVGLQG VGKTTTLVKL 130 140 150 160 170 180 AKWIKEKYKK KILTVSTDIY RAAAIKQLQI LSDQVKIDFY LSDTTQTPIN ITKEAIEHAK 190 200 210 220 230 240 LKLYDLLLID TAGRLHINTE MMNEINTIQN ISKPIETLLI VDSMMGQDAI NIAKKFSASL 250 260 270 280 290 300 SISGIVITKT DSDARSGVAL SIRHITGKPI KFIGTGEKLH QLEPFHPERI ADRILGMNQV 310 320 330 340 350 360 ISLIKDIEEK VNQSQVKNLT KKFRKGDDFN LNDFLIQLKE MKNMGNLNYF IEKFSKNKIL 370 380 390 400 410 420 SNNPLLGENK NTLNRIEAII YSMTHKERMH PIIIKGSRKR RIALGSGTKI QDVNKLLKNF 430 440 450 DNIKKIMKKI KKGGIGKMIR NISNILPKNF

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67932.1.
RefSeq	NP_660721.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9F7.
SMR	Q8K9F7. Positions 366-431.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg380.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67932; AAM67932; BUsg_380.
GeneID	1005777.
KEGG	bas:BUsg380.
PATRIC	21247578. VBIBucAph100086_0394.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0541.
KO	K03106.
OMA	GQQVVKI.
ProtClustDB	CLSK315010.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-434-MONOMER.
Family and domain databases
Gene3D	1.10.260.30. 1 hit.
HAMAP	MF_00306. SRP54.
InterPro	IPR003593. AAA+_ATPase. IPR013822. Signal_recog_particl_SRP54_hlx. IPR004125. Signal_recog_particle_SRP54_M. IPR022941. SRP54. IPR000897. SRP54_GTPase_dom. IPR004780. SRP_Ffh. [Graphical view]
PANTHER	PTHR11564:SF7. PTHR11564:SF7. 1 hit.
Pfam	PF00448. SRP54. 1 hit. PF02881. SRP54_N. 1 hit. PF02978. SRP_SPB. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00962. SRP54. 1 hit. SM00963. SRP54_N. 1 hit. [Graphical view]
SUPFAM	SSF47446. Signal_recog_particle_SRP54_M. 1 hit. SSF47364. SRP54. 1 hit.
TIGRFAMs	TIGR00959. ffh. 1 hit.
PROSITE	PS00300. SRP54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRP54_BUCAP

Accession

Primary (citable) accession number: Q8K9F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program