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Q8K9F7 (SRP54_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Signal recognition particle protein
Alternative name(s):
Fifty-four homolog
Gene names
Name:ffh
Ordered Locus Names:BUsg_380
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components By similarity. HAMAP-Rule MF_00306

Subunit structure

Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule By similarity.

Subcellular location

Cytoplasm By similarity. Note: The SRP-RNC complex is targeted to the cytoplasmic membrane By similarity. HAMAP-Rule MF_00306

Domain

Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC By similarity. HAMAP-Rule MF_00306

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450Signal recognition particle protein HAMAP-Rule MF_00306
PRO_0000101151

Regions

Nucleotide binding107 – 1148GTP By similarity
Nucleotide binding190 – 1945GTP By similarity
Nucleotide binding248 – 2514GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9F7 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 4A96BAFB8787D685

FASTA45051,017
        10         20         30         40         50         60 
MFNNLTERLS QSLRKIINKG RLTEENIKDT IREVRKALLE ADVTLSVIKK FIQNVSKKAI 

        70         80         90        100        110        120 
GHEINKSLTP GQEFIKIVKN ELILSMGEKN HDLNLSIQPP AIILVVGLQG VGKTTTLVKL 

       130        140        150        160        170        180 
AKWIKEKYKK KILTVSTDIY RAAAIKQLQI LSDQVKIDFY LSDTTQTPIN ITKEAIEHAK 

       190        200        210        220        230        240 
LKLYDLLLID TAGRLHINTE MMNEINTIQN ISKPIETLLI VDSMMGQDAI NIAKKFSASL 

       250        260        270        280        290        300 
SISGIVITKT DSDARSGVAL SIRHITGKPI KFIGTGEKLH QLEPFHPERI ADRILGMNQV 

       310        320        330        340        350        360 
ISLIKDIEEK VNQSQVKNLT KKFRKGDDFN LNDFLIQLKE MKNMGNLNYF IEKFSKNKIL 

       370        380        390        400        410        420 
SNNPLLGENK NTLNRIEAII YSMTHKERMH PIIIKGSRKR RIALGSGTKI QDVNKLLKNF 

       430        440        450 
DNIKKIMKKI KKGGIGKMIR NISNILPKNF 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67932.1.
RefSeqNP_660721.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9F7.
SMRQ8K9F7. Positions 366-431.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg380.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67932; AAM67932; BUsg_380.
GeneID1005777.
KEGGbas:BUsg380.
PATRIC21247578. VBIBucAph100086_0394.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0541.
KOK03106.
OMADPKSQFI.
OrthoDBEOG62K1ZH.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-398-MONOMER.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR000897. SRP54_GTPase_dom.
IPR004780. SRP_Ffh.
[Graphical view]
PANTHERPTHR11564:SF7. PTHR11564:SF7. 1 hit.
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47364. SSF47364. 1 hit.
SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00959. ffh. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSRP54_BUCAP
AccessionPrimary (citable) accession number: Q8K9F7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names