ID   TRMD_BUCAP              Reviewed;         262 AA.
AC   Q8K9F4;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 43.
DE   RecName: Full=tRNA (guanine-N(1)-)-methyltransferase;
DE            EC=2.1.1.31;
DE   AltName: Full=M1G-methyltransferase;
DE   AltName: Full=tRNA [GM37] methyltransferase;
GN   Name=trmD; OrderedLocusNames=BUsg_383;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Specifically methylates guanosine-37 in various tRNAs
CC       (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(1)-methylguanine.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase trmD family.
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DR   EMBL; AE013218; AAM67935.1; -; Genomic_DNA.
DR   RefSeq; NP_660724.1; -.
DR   HSSP; P43912; 1UAJ.
DR   GeneID; 1005774; -.
DR   GenomeReviews; AE013218_GR; BUsg_383.
DR   KEGG; bas:BUsg383; -.
DR   HOGENOM; Q8K9F4; -.
DR   OMA; Q8K9F4; HRSVDDT.
DR   BioCyc; BAPH198804:BUSG383-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0009019; F:tRNA (guanine-N1-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_00605; -; 1.
DR   InterPro; IPR016009; tRNA_m1G_MeTrfase.
DR   InterPro; IPR002649; tRNA_m1G_MeTrfase_bac.
DR   Pfam; PF01746; tRNA_m1G_MT; 1.
DR   PIRSF; PIRSF000386; tRNA_mtase; 1.
DR   ProDom; PD004978; tRNA_m1G_mtfrase; 1.
DR   TIGRFAMs; TIGR00088; trmD; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Methyltransferase;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing.
FT   CHAIN         1    262       tRNA (guanine-N(1)-)-methyltransferase.
FT                                /FTId=PRO_0000060347.
FT   REGION      149    154       S-adenosyl-L-methionine binding (By
FT                                similarity).
FT   BINDING     129    129       S-adenosyl-L-methionine; via amide
FT                                nitrogen (By similarity).
SQ   SEQUENCE   262 AA;  30500 MW;  653A65B1663A04CA CRC64;
     MELNKHDQTK TKYDNTLIWF CIITIFPEMF YAITNYGVTG KAIRKNIINI KFFNPRDFTD
     NKYKSVDDRP YGGGPGMLMS AKPLYLAIKN AKNLLKSAIV IYLSPQGKRL NQKKILEIIK
     NKKIIFVCGR YEGIDQRIID CQVDEEWSIG DYILTGGELA AMVAIDSISR FIPGVIKKKQ
     SVQEDSFFNG LLDYPHYTRP KIIEKMKVPE ILLSGNHDKI RLWRLKKSLE KTWTKRPDLL
     KNKILKKEEK ILLNELKKIN KR
//