Reviewed, UniProtKB/Swiss-Prot Q8K9F4 (TRMD_BUCAP)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BUsg_383

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060347
Regions
	Region	149 – 154	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	129	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9F4-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 653A65B1663A04CA	FASTA	262	30,500
	10 20 30 40 50 60 MELNKHDQTK TKYDNTLIWF CIITIFPEMF YAITNYGVTG KAIRKNIINI KFFNPRDFTD 70 80 90 100 110 120 NKYKSVDDRP YGGGPGMLMS AKPLYLAIKN AKNLLKSAIV IYLSPQGKRL NQKKILEIIK 130 140 150 160 170 180 NKKIIFVCGR YEGIDQRIID CQVDEEWSIG DYILTGGELA AMVAIDSISR FIPGVIKKKQ 190 200 210 220 230 240 SVQEDSFFNG LLDYPHYTRP KIIEKMKVPE ILLSGNHDKI RLWRLKKSLE KTWTKRPDLL 250 260 KNKILKKEEK ILLNELKKIN KR

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67935.1.
RefSeq	NP_660724.1.
3D structure databases
HSSP	HSSP built from PDB template 1UAJ based on UniProtKB P43912.
ModBase	Search...
Genome annotation databases
GeneID	1005774.
GenomeReviews	Gene locus BUsg_383 in contig AE013218_GR.
KEGG	bas:BUsg383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9F4.
OMA	Q8K9F4. HRSVDDT.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG383-MON.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BUCAP

Accession

Primary (citable) accession number: Q8K9F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)