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Q8K9E1 (SYQ_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase
EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:BUsg_398
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera ›

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAM67949.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 562562Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_0000195828

Regions

Motif35 – 4511"HIGH" region HAMAP-Rule MF_00126
Motif269 – 2735"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2721ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9E1 [UniParc].

Last modified November 8, 2002. Version 2.
Checksum: A2379AEF06758583

FASTA56266,769
        10         20         30         40         50         60 
MNNKIEKNDN NFIYKIINED FKKNKNLLLH TRFPPEPNGY LHIGHAKSIC LNFELANVYN 

        70         80         90        100        110        120 
GYCNLRFDDT NPVKENIKYI NSIKNDIKWL GYQWHKKVRY SSEYFPILYE YAKELIQKGL 

       130        140        150        160        170        180 
AYVDELTKEE IREYRGTLST VGKNSPYRNR SITENLKLFE KMKKGEFKEG EACLRAKINM 

       190        200        210        220        230        240 
SSSFIVMRDP VLYRIIFSTH HQTKKKWCIY PTYDFAHCLS DSIEGITHSF CTLEFQDNKN 

       250        260        270        280        290        300 
LYNWILKNTS IKHYSKQYEF SRLNLEYSIL SKRKISILIE KKIVNGWDDP RILTISGLRK 

       310        320        330        340        350        360 
KGYTASSIRD FCHRIGITKQ NNLIEFSMLE YCIRKELNKK AIRTMAVLEP IKIFLYNIDN 

       370        380        390        400        410        420 
DHEEKLIVPN HPKHLHLGTH EIIFTNTIYI EREDFKEKYD KKYKRLKIGE EVRLRYAYII 

       430        440        450        460        470        480 
KAEKIEKDES GNIIKIICFC DINSLGKKPI NRKNPAVIHW IAVKNSFPAK FKLYNQLFKI 

       490        500        510        520        530        540 
KNPDQEKNFL SYLNSNSLIV KNGFVEKKIA QKIIKKMSNN FIKLFFQFER TGYFCVDEVD 

       550        560 
SKENKLVFNR TVSLKDSWNL KK

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE013218 Genomic DNA. Translation: AAM67949.1. Different initiation.
RefSeqNP_660738.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9E1.
SMRQ8K9E1. Positions 10-557.
ModBaseSearch...

Protein-protein interaction databases

STRING198804.BUsg398.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67949; AAM67949; BUsg_398.
GeneID1005762.
KEGGbas:BUsg398.
PATRIC21247622. VBIBucAph100086_0411.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
KOK01886.
OMARMPTIAG.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-451-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth_Ib.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_BUCAP
AccessionPrimary (citable) accession number: Q8K9E1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents