Q8K9D8 (QUED_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-carboxy-5,6,7,8-tetrahydropterin synthase
Short name=CPH4 synthase
EC=4.1.2.50

Alternative name(s):
Queuosine biosynthesis protein QueD

Gene names

Name:	queD
Ordered Locus Names:	BUsg_402

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde By similarity.
Catalytic activity	7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Miscellaneous	The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit By similarity.
Sequence similarities	Belongs to the PTPS family. QueD subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	6-carboxy-5,6,7,8-tetrahydropterin synthase		PRO_0000057921
Sites
	Active site	26	1	Proton acceptor By similarity
	Active site	70	1	Charge relay system By similarity
	Active site	109	1	Charge relay system By similarity
	Metal binding	15	1	Zinc By similarity
	Metal binding	30	1	Zinc By similarity
	Metal binding	32	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9D8 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 38840ED621058DB3	FASTA	120	14,273
	10 20 30 40 50 60 MKTIIFKDFQ FEAAHYLPYV PKMHKCRRLH GHSFFVRLEL KDKINEKNGW IIDYAEIKLA 70 80 90 100 110 120 FQPIYDQLDH HFLNDIPGLE NPTSEILAKW IWHRLKPKLS ILNTIIIKET CTSGCIYQGF

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67953.1.
RefSeq	NP_660742.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9D8.
SMR	Q8K9D8. Positions 5-119.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg402.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67953; AAM67953; BUsg_402.
GeneID	1005758.
KEGG	bas:BUsg402.
PATRIC	21247630. VBIBucAph100086_0415.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0720.
KO	K01737.
OMA	IHETCTS.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-425-MONOMER.
UniPathway	UPA00391.
Family and domain databases
InterPro	IPR007115. 6-PTP_synth/QueD. [Graphical view]
PANTHER	PTHR12589. PTHR12589. 1 hit.
Pfam	PF01242. PTPS. 1 hit. [Graphical view]
PIRSF	PIRSF006113. PTP_synth. 1 hit.
TIGRFAMs	TIGR03367. queuosine_QueD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUED_BUCAP

Accession

Primary (citable) accession number: Q8K9D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program