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Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per subunit.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per subunit.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), Putative sulfite reductase [NADPH] hemoprotein beta-component (cysI)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei325FADUniRule annotation1
Binding sitei359FAD; via carbonyl oxygenUniRule annotation1
Binding sitei564NADPUniRule annotation1
Binding sitei602FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 79FMNUniRule annotation6
Nucleotide bindingi121 – 124FMNUniRule annotation4
Nucleotide bindingi157 – 166FMNUniRule annotation10
Nucleotide bindingi389 – 392FADUniRule annotation4
Nucleotide bindingi407 – 409FADUniRule annotation3
Nucleotide bindingi422 – 425FADUniRule annotation4
Nucleotide bindingi522 – 523NADPUniRule annotation2
Nucleotide bindingi528 – 532NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-437-MONOMER
UniPathwayiUPA00140; UER00207

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-FPUniRule annotation
Gene namesi
Name:cysJUniRule annotation
Ordered Locus Names:BUsg_413
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001999211 – 602Sulfite reductase [NADPH] flavoprotein alpha-componentAdd BLAST602

Proteomic databases

PRIDEiQ8K9D3

Interactioni

Subunit structurei

Alpha8-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg413

Structurei

3D structure databases

ProteinModelPortaliQ8K9D3
SMRiQ8K9D3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 206Flavodoxin-likeUniRule annotationAdd BLAST139
Domaini237 – 451FAD-binding FR-typeUniRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the NADPH-dependent sulphite reductase flavoprotein subunit CysJ family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EER Bacteria
COG0369 LUCA
KOiK00380
OMAiTKMDVAF
OrthoDBiPOG091H04ZI

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.360, 1 hit
HAMAPiMF_01541 CysJ, 1 hit
InterProiView protein in InterPro
IPR010199 CysJ
IPR029758 CysJ_Proteobact
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000207 SiR-FP_CysJ, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF63380 SSF63380, 1 hit
TIGRFAMsiTIGR01931 cysJ, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K9D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNKNPFNIF FPLSLDKIEN LEKIKKNCSS IQYAWLSGYF WNLANQTPSR
60 70 80 90 100
LICEKNEFFR KDNEEKIITI ISASQTGNAR QLAKRFNKYL KNENKKTNLI
110 120 130 140 150
DAADYNFKKI KNERFLILII STQGEGEPPE EALSFYKFIM SKKAPRLENL
160 170 180 190 200
HYSVFGLGDV SYNLFCQAGK DFDKRFSELG GKSLLHRLDS DIEYESNYIQ
210 220 230 240 250
WSEELLLAIN KIDVSTCSVF KKNDKKNFID KLNYTKYKPA VATVLLNQKI
260 270 280 290 300
TGRNSTKDVH HIELDITNSN IVYTPGDALG VWYQNSSQLI KQILKLLSIR
310 320 330 340 350
ISDKVKVKDK IITIFEALKK NFELTTNTKH IIQKYTDVTQ NKFLKKIISD
360 370 380 390 400
NKKLNNYVKK TPLLKMIYDH PKKLSSQQLI SILRPLKPRL YSISSSQSEM
410 420 430 440 450
NDEVHITVGV VKKQISGTIH LGGSSSYLSQ FLKIDDSVKI FVEEKSNFRL
460 470 480 490 500
PENKDVPIIM IGSGTGIAPF RAFIQQRDND KATGKNWIFF GNPNFTEDFL
510 520 530 540 550
YQLEWQKYLK KKLLTKMSLA WSRDQKEKIY VQDKIRENGK ELWEWVNQGA
560 570 580 590 600
QIYVCGNASK MAKDVEKELL DVFSKNGSMD IEESSEFLNN LRITRRYQRD

VY
Length:602
Mass (Da):69,930
Last modified:October 1, 2002 - v1
Checksum:iFA3793147889B7B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA Translation: AAM67958.1
RefSeqiWP_011053925.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM67958; AAM67958; BUsg_413
KEGGibas:BUsg_413

Similar proteinsi

Entry informationi

Entry nameiCYSJ_BUCAP
AccessioniPrimary (citable) accession number: Q8K9D3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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