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Q8K9D3

- CYSJ_BUCAP

UniProt

Q8K9D3 - CYSJ_BUCAP

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Protein
Sulfite reductase [NADPH] flavoprotein alpha-component
Gene
cysJ, BUsg_413
Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Binds 1 FAD per subunit By similarity.UniRule annotation
Binds 1 FMN per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei492 – 4921NADP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 785FMN By similarity
Nucleotide bindingi121 – 1266FMN By similarity
Nucleotide bindingi154 – 18532FMN By similarity
Add
BLAST
Nucleotide bindingi389 – 3924FAD By similarity
Nucleotide bindingi423 – 4253FAD By similarity
Nucleotide bindingi522 – 5309NADP By similarity

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-436-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-component (EC:1.8.1.2)
Short name:
SiR-FP
Gene namesi
Name:cysJ
Ordered Locus Names:BUsg_413
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation
PRO_0000199921Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Protein-protein interaction databases

STRINGi198804.BUsg413.

Structurei

3D structure databases

ProteinModelPortaliQ8K9D3.
SMRiQ8K9D3. Positions 234-602.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 206139Flavodoxin-like
Add
BLAST
Domaini237 – 451215FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0369.
KOiK00380.
OMAiHEFLQSK.
OrthoDBiEOG6CVV7G.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_01541. CysJ.
InterProiIPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01931. cysJ. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9D3-1 [UniParc]FASTAAdd to Basket

« Hide

MKNKNPFNIF FPLSLDKIEN LEKIKKNCSS IQYAWLSGYF WNLANQTPSR    50
LICEKNEFFR KDNEEKIITI ISASQTGNAR QLAKRFNKYL KNENKKTNLI 100
DAADYNFKKI KNERFLILII STQGEGEPPE EALSFYKFIM SKKAPRLENL 150
HYSVFGLGDV SYNLFCQAGK DFDKRFSELG GKSLLHRLDS DIEYESNYIQ 200
WSEELLLAIN KIDVSTCSVF KKNDKKNFID KLNYTKYKPA VATVLLNQKI 250
TGRNSTKDVH HIELDITNSN IVYTPGDALG VWYQNSSQLI KQILKLLSIR 300
ISDKVKVKDK IITIFEALKK NFELTTNTKH IIQKYTDVTQ NKFLKKIISD 350
NKKLNNYVKK TPLLKMIYDH PKKLSSQQLI SILRPLKPRL YSISSSQSEM 400
NDEVHITVGV VKKQISGTIH LGGSSSYLSQ FLKIDDSVKI FVEEKSNFRL 450
PENKDVPIIM IGSGTGIAPF RAFIQQRDND KATGKNWIFF GNPNFTEDFL 500
YQLEWQKYLK KKLLTKMSLA WSRDQKEKIY VQDKIRENGK ELWEWVNQGA 550
QIYVCGNASK MAKDVEKELL DVFSKNGSMD IEESSEFLNN LRITRRYQRD 600
VY 602
Length:602
Mass (Da):69,930
Last modified:October 1, 2002 - v1
Checksum:iFA3793147889B7B3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67958.1.
RefSeqiNP_660747.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67958; AAM67958; BUsg_413.
GeneIDi1005748.
KEGGibas:BUsg413.
PATRICi21247670. VBIBucAph100086_0435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. Translation: AAM67958.1 .
RefSeqi NP_660747.1. NC_004061.1.

3D structure databases

ProteinModelPortali Q8K9D3.
SMRi Q8K9D3. Positions 234-602.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg413.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67958 ; AAM67958 ; BUsg_413 .
GeneIDi 1005748.
KEGGi bas:BUsg413.
PATRICi 21247670. VBIBucAph100086_0435.

Phylogenomic databases

eggNOGi COG0369.
KOi K00380.
OMAi HEFLQSK.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00207 .
BioCyci BAPH198804:GHMG-436-MONOMER.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPi MF_01541. CysJ.
InterProi IPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsi TIGR01931. cysJ. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiCYSJ_BUCAP
AccessioniPrimary (citable) accession number: Q8K9D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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