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Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per subunit.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per subunit.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), Putative sulfite reductase [NADPH] hemoprotein beta-component (cysI)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei325 – 3251FADUniRule annotation
Binding sitei359 – 3591FAD; via carbonyl oxygenUniRule annotation
Binding sitei564 – 5641NADPUniRule annotation
Binding sitei602 – 6021FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 796FMNUniRule annotation
Nucleotide bindingi121 – 1244FMNUniRule annotation
Nucleotide bindingi157 – 16610FMNUniRule annotation
Nucleotide bindingi389 – 3924FADUniRule annotation
Nucleotide bindingi407 – 4093FADUniRule annotation
Nucleotide bindingi422 – 4254FADUniRule annotation
Nucleotide bindingi522 – 5232NADPUniRule annotation
Nucleotide bindingi528 – 5325NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-436-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-FPUniRule annotation
Gene namesi
Name:cysJUniRule annotation
Ordered Locus Names:BUsg_413
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Sulfite reductase [NADPH] flavoprotein alpha-componentPRO_0000199921Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg413.

Structurei

3D structure databases

ProteinModelPortaliQ8K9D3.
SMRiQ8K9D3. Positions 234-602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 206139Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini237 – 451215FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH-dependent sulphite reductase flavoprotein subunit CysJ family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107EER. Bacteria.
COG0369. LUCA.
KOiK00380.
OMAiGVWYEND.
OrthoDBiEOG6CVV7G.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_01541. CysJ.
InterProiIPR010199. CysJ.
IPR029758. CysJ_Proteobact.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01931. cysJ. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNKNPFNIF FPLSLDKIEN LEKIKKNCSS IQYAWLSGYF WNLANQTPSR
60 70 80 90 100
LICEKNEFFR KDNEEKIITI ISASQTGNAR QLAKRFNKYL KNENKKTNLI
110 120 130 140 150
DAADYNFKKI KNERFLILII STQGEGEPPE EALSFYKFIM SKKAPRLENL
160 170 180 190 200
HYSVFGLGDV SYNLFCQAGK DFDKRFSELG GKSLLHRLDS DIEYESNYIQ
210 220 230 240 250
WSEELLLAIN KIDVSTCSVF KKNDKKNFID KLNYTKYKPA VATVLLNQKI
260 270 280 290 300
TGRNSTKDVH HIELDITNSN IVYTPGDALG VWYQNSSQLI KQILKLLSIR
310 320 330 340 350
ISDKVKVKDK IITIFEALKK NFELTTNTKH IIQKYTDVTQ NKFLKKIISD
360 370 380 390 400
NKKLNNYVKK TPLLKMIYDH PKKLSSQQLI SILRPLKPRL YSISSSQSEM
410 420 430 440 450
NDEVHITVGV VKKQISGTIH LGGSSSYLSQ FLKIDDSVKI FVEEKSNFRL
460 470 480 490 500
PENKDVPIIM IGSGTGIAPF RAFIQQRDND KATGKNWIFF GNPNFTEDFL
510 520 530 540 550
YQLEWQKYLK KKLLTKMSLA WSRDQKEKIY VQDKIRENGK ELWEWVNQGA
560 570 580 590 600
QIYVCGNASK MAKDVEKELL DVFSKNGSMD IEESSEFLNN LRITRRYQRD

VY
Length:602
Mass (Da):69,930
Last modified:October 1, 2002 - v1
Checksum:iFA3793147889B7B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67958.1.
RefSeqiWP_011053925.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67958; AAM67958; BUsg_413.
KEGGibas:BUsg_413.
PATRICi21247670. VBIBucAph100086_0435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67958.1.
RefSeqiWP_011053925.1. NC_004061.1.

3D structure databases

ProteinModelPortaliQ8K9D3.
SMRiQ8K9D3. Positions 234-602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67958; AAM67958; BUsg_413.
KEGGibas:BUsg_413.
PATRICi21247670. VBIBucAph100086_0435.

Phylogenomic databases

eggNOGiENOG4107EER. Bacteria.
COG0369. LUCA.
KOiK00380.
OMAiGVWYEND.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.
BioCyciBAPH198804:GHMG-436-MONOMER.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_01541. CysJ.
InterProiIPR010199. CysJ.
IPR029758. CysJ_Proteobact.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01931. cysJ. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiCYSJ_BUCAP
AccessioniPrimary (citable) accession number: Q8K9D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: January 20, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.