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Q8K9D3

- CYSJ_BUCAP

UniProt

Q8K9D3 - CYSJ_BUCAP

Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.UniRule annotation

    Catalytic activityi

    H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

    Cofactori

    Binds 1 FAD per subunit.UniRule annotation
    Binds 1 FMN per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei492 – 4921NADPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi74 – 785FMNUniRule annotation
    Nucleotide bindingi121 – 1266FMNUniRule annotation
    Nucleotide bindingi154 – 18532FMNUniRule annotationAdd
    BLAST
    Nucleotide bindingi389 – 3924FADUniRule annotation
    Nucleotide bindingi423 – 4253FADUniRule annotation
    Nucleotide bindingi522 – 5309NADPUniRule annotation

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. sulfite reductase (NADPH) activity Source: UniProtKB-EC

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
    3. sulfate assimilation Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-436-MONOMER.
    UniPathwayiUPA00140; UER00207.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
    Short name:
    SiR-FPUniRule annotation
    Gene namesi
    Name:cysJUniRule annotation
    Ordered Locus Names:BUsg_413
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 602602Sulfite reductase [NADPH] flavoprotein alpha-componentPRO_0000199921Add
    BLAST

    Interactioni

    Subunit structurei

    Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

    Protein-protein interaction databases

    STRINGi198804.BUsg413.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K9D3.
    SMRiQ8K9D3. Positions 234-602.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini68 – 206139Flavodoxin-likeUniRule annotationAdd
    BLAST
    Domaini237 – 451215FAD-binding FR-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding FR-type domain.UniRule annotation
    Contains 1 flavodoxin-like domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0369.
    KOiK00380.
    OMAiHEFLQSK.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPiMF_01541. CysJ.
    InterProiIPR010199. CysJ.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    TIGRFAMsiTIGR01931. cysJ. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K9D3-1 [UniParc]FASTAAdd to Basket

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    MKNKNPFNIF FPLSLDKIEN LEKIKKNCSS IQYAWLSGYF WNLANQTPSR    50
    LICEKNEFFR KDNEEKIITI ISASQTGNAR QLAKRFNKYL KNENKKTNLI 100
    DAADYNFKKI KNERFLILII STQGEGEPPE EALSFYKFIM SKKAPRLENL 150
    HYSVFGLGDV SYNLFCQAGK DFDKRFSELG GKSLLHRLDS DIEYESNYIQ 200
    WSEELLLAIN KIDVSTCSVF KKNDKKNFID KLNYTKYKPA VATVLLNQKI 250
    TGRNSTKDVH HIELDITNSN IVYTPGDALG VWYQNSSQLI KQILKLLSIR 300
    ISDKVKVKDK IITIFEALKK NFELTTNTKH IIQKYTDVTQ NKFLKKIISD 350
    NKKLNNYVKK TPLLKMIYDH PKKLSSQQLI SILRPLKPRL YSISSSQSEM 400
    NDEVHITVGV VKKQISGTIH LGGSSSYLSQ FLKIDDSVKI FVEEKSNFRL 450
    PENKDVPIIM IGSGTGIAPF RAFIQQRDND KATGKNWIFF GNPNFTEDFL 500
    YQLEWQKYLK KKLLTKMSLA WSRDQKEKIY VQDKIRENGK ELWEWVNQGA 550
    QIYVCGNASK MAKDVEKELL DVFSKNGSMD IEESSEFLNN LRITRRYQRD 600
    VY 602
    Length:602
    Mass (Da):69,930
    Last modified:October 1, 2002 - v1
    Checksum:iFA3793147889B7B3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67958.1.
    RefSeqiNP_660747.1. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM67958; AAM67958; BUsg_413.
    GeneIDi1005748.
    KEGGibas:BUsg413.
    PATRICi21247670. VBIBucAph100086_0435.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM67958.1 .
    RefSeqi NP_660747.1. NC_004061.1.

    3D structure databases

    ProteinModelPortali Q8K9D3.
    SMRi Q8K9D3. Positions 234-602.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg413.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM67958 ; AAM67958 ; BUsg_413 .
    GeneIDi 1005748.
    KEGGi bas:BUsg413.
    PATRICi 21247670. VBIBucAph100086_0435.

    Phylogenomic databases

    eggNOGi COG0369.
    KOi K00380.
    OMAi HEFLQSK.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    UniPathwayi UPA00140 ; UER00207 .
    BioCyci BAPH198804:GHMG-436-MONOMER.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPi MF_01541. CysJ.
    InterProi IPR010199. CysJ.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    TIGRFAMsi TIGR01931. cysJ. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiCYSJ_BUCAP
    AccessioniPrimary (citable) accession number: Q8K9D3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3