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Q8K9D3

- CYSJ_BUCAP

UniProt

Q8K9D3 - CYSJ_BUCAP

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Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per subunit.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei492 – 4921NADPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 785FMNUniRule annotation
Nucleotide bindingi121 – 1266FMNUniRule annotation
Nucleotide bindingi154 – 18532FMNUniRule annotationAdd
BLAST
Nucleotide bindingi389 – 3924FADUniRule annotation
Nucleotide bindingi423 – 4253FADUniRule annotation
Nucleotide bindingi522 – 5309NADPUniRule annotation

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-436-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-FPUniRule annotation
Gene namesi
Name:cysJUniRule annotation
Ordered Locus Names:BUsg_413
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Sulfite reductase [NADPH] flavoprotein alpha-componentPRO_0000199921Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg413.

Structurei

3D structure databases

ProteinModelPortaliQ8K9D3.
SMRiQ8K9D3. Positions 234-602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 206139Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini237 – 451215FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0369.
KOiK00380.
OMAiHEFLQSK.
OrthoDBiEOG6CVV7G.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_01541. CysJ.
InterProiIPR010199. CysJ.
IPR029758. CysJ_Proteobact.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01931. cysJ. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9D3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNKNPFNIF FPLSLDKIEN LEKIKKNCSS IQYAWLSGYF WNLANQTPSR
60 70 80 90 100
LICEKNEFFR KDNEEKIITI ISASQTGNAR QLAKRFNKYL KNENKKTNLI
110 120 130 140 150
DAADYNFKKI KNERFLILII STQGEGEPPE EALSFYKFIM SKKAPRLENL
160 170 180 190 200
HYSVFGLGDV SYNLFCQAGK DFDKRFSELG GKSLLHRLDS DIEYESNYIQ
210 220 230 240 250
WSEELLLAIN KIDVSTCSVF KKNDKKNFID KLNYTKYKPA VATVLLNQKI
260 270 280 290 300
TGRNSTKDVH HIELDITNSN IVYTPGDALG VWYQNSSQLI KQILKLLSIR
310 320 330 340 350
ISDKVKVKDK IITIFEALKK NFELTTNTKH IIQKYTDVTQ NKFLKKIISD
360 370 380 390 400
NKKLNNYVKK TPLLKMIYDH PKKLSSQQLI SILRPLKPRL YSISSSQSEM
410 420 430 440 450
NDEVHITVGV VKKQISGTIH LGGSSSYLSQ FLKIDDSVKI FVEEKSNFRL
460 470 480 490 500
PENKDVPIIM IGSGTGIAPF RAFIQQRDND KATGKNWIFF GNPNFTEDFL
510 520 530 540 550
YQLEWQKYLK KKLLTKMSLA WSRDQKEKIY VQDKIRENGK ELWEWVNQGA
560 570 580 590 600
QIYVCGNASK MAKDVEKELL DVFSKNGSMD IEESSEFLNN LRITRRYQRD

VY
Length:602
Mass (Da):69,930
Last modified:October 1, 2002 - v1
Checksum:iFA3793147889B7B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67958.1.
RefSeqiNP_660747.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67958; AAM67958; BUsg_413.
GeneIDi1005748.
KEGGibas:BUsg413.
PATRICi21247670. VBIBucAph100086_0435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67958.1 .
RefSeqi NP_660747.1. NC_004061.1.

3D structure databases

ProteinModelPortali Q8K9D3.
SMRi Q8K9D3. Positions 234-602.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198804.BUsg413.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM67958 ; AAM67958 ; BUsg_413 .
GeneIDi 1005748.
KEGGi bas:BUsg413.
PATRICi 21247670. VBIBucAph100086_0435.

Phylogenomic databases

eggNOGi COG0369.
KOi K00380.
OMAi HEFLQSK.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00207 .
BioCyci BAPH198804:GHMG-436-MONOMER.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPi MF_01541. CysJ.
InterProi IPR010199. CysJ.
IPR029758. CysJ_Proteobact.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsi TIGR01931. cysJ. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiCYSJ_BUCAP
AccessioniPrimary (citable) accession number: Q8K9D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

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