ID   TYSY_BUCAP              Reviewed;         264 AA.
AC   Q8K9C3;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 45.
DE   RecName: Full=Thymidylate synthase;
DE            Short=TS;
DE            Short=TSase;
DE            EC=2.1.1.45;
GN   Name=thyA; OrderedLocusNames=BUsg_425;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Provides the sole de novo source of dTMP for DNA
CC       biosynthesis (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP =
CC       dihydrofolate + dTMP.
CC   -!- PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the thymidylate synthase family. Bacterial-
CC       type thyA subfamily.
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DR   EMBL; AE013218; AAM67968.1; -; Genomic_DNA.
DR   RefSeq; NP_660757.1; -.
DR   HSSP; P00470; 1F4G.
DR   GeneID; 1005738; -.
DR   GenomeReviews; AE013218_GR; BUsg_425.
DR   KEGG; bas:BUsg425; -.
DR   HOGENOM; Q8K9C3; -.
DR   OMA; Q8K9C3; LGPVYGH.
DR   BioCyc; BAPH198804:BUSG425-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004799; F:thymidylate synthase activity; IEA:HAMAP.
DR   GO; GO:0006231; P:dTMP biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00008; -; 1.
DR   InterPro; IPR000398; Thymidylat_synth_C.
DR   Gene3D; G3DSA:3.30.572.10; Thymidylat_synth_C; 1.
DR   PANTHER; PTHR11549:SF2; Thymidylat_synth_C; 1.
DR   Pfam; PF00303; Thymidylat_synt; 1.
DR   PRINTS; PR00108; THYMDSNTHASE.
DR   ProDom; PD001180; Thymidylat_synth; 1.
DR   TIGRFAMs; TIGR03284; thym_sym; 1.
DR   PROSITE; PS00091; THYMIDYLATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Methyltransferase;
KW   Nucleotide biosynthesis; Transferase.
FT   CHAIN         1    264       Thymidylate synthase.
FT                                /FTId=PRO_0000140945.
FT   ACT_SITE    146    146       By similarity.
SQ   SEQUENCE   264 AA;  30716 MW;  355E01002E2BF15A CRC64;
     MKQYLDLIKK IIKNGNSKKD RTGTGTLSIF GHHMKFDLKK GFPLITTKKC HIPSIIHELL
     WFLRGETNIK YLNENKISIW DNWADKFGDL GPIYGKQWRS WDTSNGKKID QIKNVLKQIK
     EDPDSRRILV SSWNVGEIDK MSLAPCHILF QFYVLKKKLS CQLYQRSCDV FLGLPFNIAS
     YAILIHMIAQ QCNLQVGEFL WTGGDVHLYK NHVELAKKQI LRIPKKLPEL IILKKPQSLF
     QYSFEDFKIV GYKPYSLIKG KISV
//