Q8K9C3 (TYSY_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BUsg_425

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000140945
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9C3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 355E01002E2BF15A	FASTA	264	30,716
	10 20 30 40 50 60 MKQYLDLIKK IIKNGNSKKD RTGTGTLSIF GHHMKFDLKK GFPLITTKKC HIPSIIHELL 70 80 90 100 110 120 WFLRGETNIK YLNENKISIW DNWADKFGDL GPIYGKQWRS WDTSNGKKID QIKNVLKQIK 130 140 150 160 170 180 EDPDSRRILV SSWNVGEIDK MSLAPCHILF QFYVLKKKLS CQLYQRSCDV FLGLPFNIAS 190 200 210 220 230 240 YAILIHMIAQ QCNLQVGEFL WTGGDVHLYK NHVELAKKQI LRIPKKLPEL IILKKPQSLF 250 260 QYSFEDFKIV GYKPYSLIKG KISV

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67968.1.
RefSeq	NP_660757.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9C3.
SMR	Q8K9C3. Positions 1-264.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg425.
Proteomic databases
PRIDE	Q8K9C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67968; AAM67968; BUsg_425.
GeneID	1005738.
KEGG	bas:BUsg425.
PATRIC	21247698. VBIBucAph100086_0449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
KO	K00560.
OMA	DTNVAYL.
ProtClustDB	PRK01827.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-470-MONOMER.
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BUCAP

Accession

Primary (citable) accession number: Q8K9C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program