Reviewed, UniProtKB/Swiss-Prot Q8K9C3 (TYSY_BUCAP)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BUsg_425

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140945
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9C3-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 355E01002E2BF15A	FASTA	264	30,716
	10 20 30 40 50 60 MKQYLDLIKK IIKNGNSKKD RTGTGTLSIF GHHMKFDLKK GFPLITTKKC HIPSIIHELL 70 80 90 100 110 120 WFLRGETNIK YLNENKISIW DNWADKFGDL GPIYGKQWRS WDTSNGKKID QIKNVLKQIK 130 140 150 160 170 180 EDPDSRRILV SSWNVGEIDK MSLAPCHILF QFYVLKKKLS CQLYQRSCDV FLGLPFNIAS 190 200 210 220 230 240 YAILIHMIAQ QCNLQVGEFL WTGGDVHLYK NHVELAKKQI LRIPKKLPEL IILKKPQSLF 250 260 QYSFEDFKIV GYKPYSLIKG KISV

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM67968.1.
RefSeq	NP_660757.1.
3D structure databases
HSSP	HSSP built from PDB template 1F4G based on UniProtKB P00470.
ModBase	Search...
Genome annotation databases
GeneID	1005738.
GenomeReviews	Gene locus BUsg_425 in contig AE013218_GR.
KEGG	bas:BUsg425.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K9C3.
OMA	Q8K9C3. LGPVYGH.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG425-MON.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BUCAP

Accession

Primary (citable) accession number: Q8K9C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)