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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N6-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N6-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi11Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi156Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi160Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi163Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-450-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:BUsg_426
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001417311 – 439tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST439

Proteomic databases

PRIDEiQ8K9C2

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg426

Structurei

3D structure databases

ProteinModelPortaliQ8K9C2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 119MTTase N-terminalUniRule annotationAdd BLAST118
Domaini377 – 439TRAMUniRule annotationAdd BLAST63

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIW Bacteria
COG0621 LUCA
KOiK06168
OMAiKVCEHFH
OrthoDBiPOG091H00LG

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01864 tRNA_metthiotr_MiaB, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR006463 MiaB_methiolase
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00273 (dimethylallyl)adenosine_tRNA_, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K9C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYIYIKTWG CQMNEYDSSM ITVFLEKTGR YLITKEVEKA DILILNTCSI
60 70 80 90 100
REKAQEKVFH QLGRWKKLKN KKPDVIIAVG GCVATQEGKE IFKRANYVDI
110 120 130 140 150
VFGTQTLHKL AQMIDKVEKN RRFMIDISFP QLEKFNYSLE PKKTGYTASI
160 170 180 190 200
SIMEGCNKYC SFCVVPYTRG HEISRPSDDI LLEISILAEN GVREINLLGQ
210 220 230 240 250
NVNAYRGRTF NGKICFFSEL LRLVSEIDGI DRIRFTTSNP LEFTDDIIEV
260 270 280 290 300
YQDTPKLVSF LHLPVQSGSN RILQLMKRSY TTEEYEKIIE KLILARSNIQ
310 320 330 340 350
ISSDFIVGFP SESEEDFQKT INFIKKINFD MSFSFIYSSR PGTPASEMKD
360 370 380 390 400
NIDLQEKKKR LYTLQNCINK QTMSWSRKML KSTQSVLVEG VSKKNIMELY
410 420 430
GKTENNRIVT FKGSHKMIGK FIKLKIKKVH THSLQGELI
Length:439
Mass (Da):50,787
Last modified:October 1, 2002 - v1
Checksum:i2E83E7F58CD0E5B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA Translation: AAM67969.1
RefSeqiWP_011053936.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM67969; AAM67969; BUsg_426
KEGGibas:BUsg_426

Similar proteinsi

Entry informationi

Entry nameiMIAB_BUCAP
AccessioniPrimary (citable) accession number: Q8K9C2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health