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Q8K9C2 (MIAB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB

EC=2.-.-.-
Alternative name(s):
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:BUsg_426
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864
PRO_0000141731

Regions

Domain2 – 119118MTTase N-terminal
Domain377 – 43963TRAM

Sites

Metal binding111Iron-sulfur (4Fe-4S) By similarity
Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity
Metal binding1561Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1601Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1631Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9C2 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 2E83E7F58CD0E5B8

FASTA43950,787
        10         20         30         40         50         60 
MKYIYIKTWG CQMNEYDSSM ITVFLEKTGR YLITKEVEKA DILILNTCSI REKAQEKVFH 

        70         80         90        100        110        120 
QLGRWKKLKN KKPDVIIAVG GCVATQEGKE IFKRANYVDI VFGTQTLHKL AQMIDKVEKN 

       130        140        150        160        170        180 
RRFMIDISFP QLEKFNYSLE PKKTGYTASI SIMEGCNKYC SFCVVPYTRG HEISRPSDDI 

       190        200        210        220        230        240 
LLEISILAEN GVREINLLGQ NVNAYRGRTF NGKICFFSEL LRLVSEIDGI DRIRFTTSNP 

       250        260        270        280        290        300 
LEFTDDIIEV YQDTPKLVSF LHLPVQSGSN RILQLMKRSY TTEEYEKIIE KLILARSNIQ 

       310        320        330        340        350        360 
ISSDFIVGFP SESEEDFQKT INFIKKINFD MSFSFIYSSR PGTPASEMKD NIDLQEKKKR 

       370        380        390        400        410        420 
LYTLQNCINK QTMSWSRKML KSTQSVLVEG VSKKNIMELY GKTENNRIVT FKGSHKMIGK 

       430 
FIKLKIKKVH THSLQGELI 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67969.1.
RefSeqNP_660758.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9C2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg426.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67969; AAM67969; BUsg_426.
GeneID1005737.
KEGGbas:BUsg426.
PATRIC21247700. VBIBucAph100086_0450.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0621.
KOK06168.
OMAIDAYGRD.
OrthoDBEOG6P5ZD8.
ProtClustDBPRK14325.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-449-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_BUCAP
AccessionPrimary (citable) accession number: Q8K9C2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names