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Q8K9B9 (SYL_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BUsg_429
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151988

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif620 – 6245"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6231ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9B9 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 3616484C35A366E0

FASTA861102,107
        10         20         30         40         50         60 
MEKEYCPKNI EPYVQQYWKD NKVFHVYEDD KKEKYYCLPM LPYPSGKLHM GHVRNYTISD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPIGWD AFGLPAEEAA IKNKTKPSDW TQKNIKYMKK QLQSLGFSYD 

       130        140        150        160        170        180 
WSREITTCKP DYYCWEQWFF IQLYKKKLVY KKNSFVNWCP YDKTVLANEQ VIKGCCWRCQ 

       190        200        210        220        230        240 
NKIKVKKIPQ WFIKIRNYAE SLHNDLNDLE NWPEKVKNMQ RNWIGRSKGF EIVFNVLNSN 

       250        260        270        280        290        300 
KKIKAFTNRL DLIMGATYIS ISPCHEFSVH VSKEKKEIQK FIDQKINNSA SQEDIEKIKF 

       310        320        330        340        350        360 
EGINSNMFVI HPITNKKIPV WISNFIQKEY GTNAIISVPG HNQNDWNFSI KHNLKIKYVI 

       370        380        390        400        410        420 
KNKKYKNTQL HNLFTTEKGI LYNSGEFNNL NYHNATEKIK KTLLKKKIIK EKINYKLQDW 

       430        440        450        460        470        480 
CISRQRYWGT PIPMATKKNG EIIAIPEKNL PVLLPEIKNY SDSLQKPMDS SSKWANIKIE 

       490        500        510        520        530        540 
NQDVIRETDT FDTFMESSWY YARYTCPNFN TGMIDPTASK YWLPVDQYIG GIEHATMHLI 

       550        560        570        580        590        600 
YFRFYHKLLR DFKLVELNEP VKNLICQGMV LSEAFYQFDK NNQRNWIHPS CVQVEKNLKG 

       610        620        630        640        650        660 
ETIKVDIKNK KKVIYAGMIK MSKSKNNGIE PELMINRYGA DTLRLFIMFA APIESSLEWR 

       670        680        690        700        710        720 
ESGVKGIYRF LKKIWKLVFN HIEVKKINKK VNFDILNKKQ KKMYCLLHKT IIKVSDDIGR 

       730        740        750        760        770        780 
RKSFNTAISS IMELVNELSI FKIENEEDKS IIKESLMSII KMLYPFTPHF CFRLWQYLNK 

       790        800        810        820        830        840 
NCCIDYETWP TFEKKILSSD KNTLIIQING KKQCAIEVKN HLNKEEILSY IENQSIIQKK 

       850        860 
IKNLKIIKII YIPQKVINFV V 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67972.1.
RefSeqNP_660761.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9B9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg429.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67972; AAM67972; BUsg_429.
GeneID1005734.
KEGGbas:BUsg429.
PATRIC21247708. VBIBucAph100086_0454.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-452-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BUCAP
AccessionPrimary (citable) accession number: Q8K9B9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries