Q8K9B8 (HOLA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit delta
EC=2.7.7.7

Gene names

Name:	holA
Ordered Locus Names:	BUsg_430

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the beta subunit on the DNA By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Ontologies

Keywords
Biological process	DNA replication
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	DNA polymerase III subunit delta		PRO_0000105503

Sequences

	Sequence		Length	Mass (Da)
	Q8K9B8 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 600B449B547721B3	FASTA	338	40,430
	10 20 30 40 50 60 MKIIYPEELK QKLIKKLNYF YILLGEDSIL LSKAEQLIFY FAKDKKFIEK NTINIEKSTD 70 80 90 100 110 120 WNKVINFHKS KNLFFKKTIL IINFTIKKLN LSLVKNINKI PLFKNKDILT VIKLNALSSL 130 140 150 160 170 180 IKKSTILKIL ILESEIIYCS TPYEWAFKKW LQYEIKTRNL KITQESFLLL HKYYEGNTLC 190 200 210 220 230 240 LNQILDILSI TWPNENIKIE KIKKIINQFS IFSPVNWINA IFNKNKKKAI YILDSFFKQK 250 260 270 280 290 300 YNPLILIRTL QKDLLILLNM KREKKLNINF FLKKNNISLN RSKFFANALY YIDFNNFLKI 310 320 330 IRILLQIEIK IKKEYNNSVW IQLKTLTLLL SSPMKYTP

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67973.1.
RefSeq	NP_660762.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9B8.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg430.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67973; AAM67973; BUsg_430.
GeneID	1005733.
KEGG	bas:BUsg430.
PATRIC	21247710. VBIBucAph100086_0455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1466.
KO	K02340.
OMA	NHWIESI.
ProtClustDB	CLSK316213.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-453-MONOMER.
Family and domain databases
InterPro	IPR008921. DNA_pol3_clamp-load_cplx_C. IPR010372. DNA_pol3_delta. IPR005790. DNA_polIII_delta. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF06144. DNA_pol3_delta. 1 hit. [Graphical view]
SUPFAM	SSF48019. Pol_clamp_load_C. 1 hit. SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR01128. holA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HOLA_BUCAP

Accession

Primary (citable) accession number: Q8K9B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program