Q8K9B6 (TUSA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfurtransferase TusA
EC=2.8.1.-

Alternative name(s):
tRNA 2-thiouridine synthesizing protein A

Gene names

Name:	tusA
Ordered Locus Names:	BUsg_432

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions. Interacts with IscS and stimulates its activity. Then, accepts a sulfur from IscS and transfers it in turn to TusD By similarity. HAMAP-Rule MF_00413
Subunit structure	Interacts with IscS By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the UPF0033 family. TusA subfamily.
Sequence caution	The sequence AAM67975.1 differs from that shown. Reason: Frameshift at position 2.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sulfurtransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 76	76	Sulfurtransferase TusA HAMAP-Rule MF_00413		PRO_0000159031
Sites
	Active site	15	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K9B6 [UniParc]. Last modified November 8, 2002. Version 2. Checksum: EA4CA273CEC03D7B	FASTA	76	9,206
	10 20 30 40 50 60 MEKKNIILNL IGLRCPEPIM VIRKTLRKMK KNEKILVLAD DPSTKRDIPY FCYFMEHQLL 70 DRFIHVKPYR YLLKKG

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67975.1. Frameshift.
RefSeq	NP_660764.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K9B6.
SMR	Q8K9B6. Positions 4-76.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg432.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67975; AAM67975; BUsg_432.
GeneID	1005820.
KEGG	bas:BUsg432.
PATRIC	21247714. VBIBucAph100086_0457.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0425.
KO	K04085.
OMA	KTIRNMQ.
ProtClustDB	PRK00299.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-477-MONOMER.
Family and domain databases
Gene3D	3.30.110.40. 1 hit.
HAMAP	MF_00413. Thiourid_synth_A.
InterPro	IPR022931. Sulphurtransferase_TusA. IPR001455. TusA-like. [Graphical view]
Pfam	PF01206. SirA. 1 hit. [Graphical view]
SUPFAM	SSF64307. SirA_like. 1 hit.
PROSITE	PS01148. UPF0033. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TUSA_BUCAP

Accession

Primary (citable) accession number: Q8K9B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 8, 2002
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program