Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8K9B5 (DHAS_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aspartate-semialdehyde dehydrogenase

Short name=ASA dehydrogenase
Short name=ASADH
EC=1.2.1.11
Alternative name(s):
Aspartate-beta-semialdehyde dehydrogenase
Gene names
Name:asd
Ordered Locus Names:BUsg_433
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate By similarity. HAMAP-Rule MF_02121

Catalytic activity

L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH. HAMAP-Rule MF_02121

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4. HAMAP-Rule MF_02121

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. HAMAP-Rule MF_02121

Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5. HAMAP-Rule MF_02121

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_02121

Sequence similarities

Belongs to the aspartate-semialdehyde dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 371371Aspartate-semialdehyde dehydrogenase HAMAP-Rule MF_02121
PRO_0000141364

Regions

Nucleotide binding11 – 144NADP By similarity
Nucleotide binding38 – 392NADP By similarity
Nucleotide binding167 – 1682NADP By similarity

Sites

Active site1371Acyl-thioester intermediate By similarity
Active site2761Proton acceptor By similarity
Binding site751NADP By similarity
Binding site1041Phosphate By similarity
Binding site1641Substrate By similarity
Binding site2431Substrate By similarity
Binding site2461Phosphate By similarity
Binding site2691Substrate By similarity
Binding site3521NADP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K9B5 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 079A0E1BEAE68318

FASTA37141,785
        10         20         30         40         50         60 
MTKSVGIIGW RGMVGSVLLK RMQEENDFSK IIPYFFSTSQ SGQDGPIINN ILSKNLKDAY 

        70         80         90        100        110        120 
NINLLQEMDI IITCQGSSYT EKIYPKLRNN NWQGYWIDAA STLRMEKDAT IILDPVNLNV 

       130        140        150        160        170        180 
INNALDKGIK TFVGGNCTVS LMLMALGGLF VNNLIDWVFV STYQAASGAG SRYVIELLKQ 

       190        200        210        220        230        240 
MGSLYNVVSK DLLDKSYSVL DIEKKVTQES RSKNFPLENF SVPLATSLIP WIDVEMKNGQ 

       250        260        270        280        290        300 
SREEWKGQAE TNKILNLKKK VLIDGTCVRI SSIRCHSQSF LIKLNKDISL ENIKKIIVNH 

       310        320        330        340        350        360 
NQWVDVIPNN MQKTLCNLTP SAVTDTLNIP IGRLRKLNID DRYLSAFTVG DQLLWGAAEP 

       370 
LRRMLNLLVN I 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM67976.1.
RefSeqNP_660765.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K9B5.
SMRQ8K9B5. Positions 3-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg433.

Proteomic databases

PRIDEQ8K9B5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM67976; AAM67976; BUsg_433.
GeneID1005553.
KEGGbas:BUsg433.
PATRIC21247716. VBIBucAph100086_0458.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0136.
KOK00133.
OMALASHNEW.
OrthoDBEOG67X1PS.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-456-MONOMER.
UniPathwayUPA00034; UER00016.
UPA00050; UER00463.
UPA00051; UER00464.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_02121. ASADH.
InterProIPR000319. Asp-semialdehyde_DH_CS.
IPR011534. Asp_ADH_gamma-type.
IPR012080. Asp_semialdehyde_DH.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
PIRSFPIRSF000148. ASA_dh. 1 hit.
SMARTSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR01745. asd_gamma. 1 hit.
PROSITEPS01103. ASD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHAS_BUCAP
AccessionPrimary (citable) accession number: Q8K9B5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names