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Protein

Fructose-bisphosphate aldolase class 2

Gene

fbaA

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611Glyceraldehyde 3-phosphateBy similarity
Active sitei110 – 1101Proton donorBy similarity
Metal bindingi111 – 1111Zinc 1; catalyticBy similarity
Metal bindingi145 – 1451Zinc 2By similarity
Metal bindingi175 – 1751Zinc 2By similarity
Metal bindingi227 – 2271Zinc 1; catalyticBy similarity
Binding sitei228 – 2281Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi265 – 2651Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-459-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase class 2 (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Fructose-bisphosphate aldolase class II
Gene namesi
Name:fbaA
Synonyms:fba
Ordered Locus Names:BUsg_436
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Fructose-bisphosphate aldolase class 2PRO_0000178709Add
BLAST

Proteomic databases

PRIDEiQ8K9B2.

Interactioni

Protein-protein interaction databases

STRINGi198804.BUsg436.

Structurei

3D structure databases

ProteinModelPortaliQ8K9B2.
SMRiQ8K9B2. Positions 2-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 2683Dihydroxyacetone phosphate bindingBy similarity
Regioni287 – 2904Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG69GZPB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K9B2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILNLIQPG VVTGNECQII FELAKKKKFA IPAVNCIGTD SINAVLETAS
60 70 80 90 100
RVKSPVIIQF SYGGASFIAG YKRTSSKNPE DQAIQGSISG AQHVHLMSQY
110 120 130 140 150
YQIPVILHTD HCSKEMLPWI DGLLEEGKKH YKNFGKPLFT SHMIDLSKEH
160 170 180 190 200
LKENIAICSN YLKKIKKINM ILEIELGCTG GEEDGIDNSN IDKKLLYTQP
210 220 230 240 250
KDVNYAYKKL SKISTNFTIA ASFGNVHGVY QPGNVNLKPN ILKESQEYVS
260 270 280 290 300
TKHNLKKLPL NLVFHGGSGS DLKEIHESIE YGIVKMNIDT DIQWASWKGV
310 320 330 340 350
FNFYKKNKDF LQKQIGNKNG KNIPNKKYYD PRSWIRESQE SMSKRLEKTF

KDLNAFNIL
Length:359
Mass (Da):40,638
Last modified:October 1, 2002 - v1
Checksum:iF1886D3D05238676
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67979.1.
RefSeqiNP_660768.1. NC_004061.1.
WP_011053946.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67979; AAM67979; BUsg_436.
GeneIDi1005729.
KEGGibas:BUsg436.
PATRICi21247722. VBIBucAph100086_0461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA. Translation: AAM67979.1.
RefSeqiNP_660768.1. NC_004061.1.
WP_011053946.1. NC_004061.1.

3D structure databases

ProteinModelPortaliQ8K9B2.
SMRiQ8K9B2. Positions 2-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg436.

Proteomic databases

PRIDEiQ8K9B2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67979; AAM67979; BUsg_436.
GeneIDi1005729.
KEGGibas:BUsg436.
PATRICi21247722. VBIBucAph100086_0461.

Phylogenomic databases

eggNOGiCOG0191.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG69GZPB.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciBAPH198804:GHMG-459-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiALF_BUCAP
AccessioniPrimary (citable) accession number: Q8K9B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: March 4, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.