Q8K993 (CYOA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinol oxidase subunit 2
EC=1.10.3.-

Alternative name(s):
Cytochrome o subunit 2
Cytochrome o ubiquinol oxidase subunit 2
Oxidase BO(3) subunit 2
Ubiquinol oxidase polypeptide II

Gene names

Name:	cyoA
Ordered Locus Names:	BUsg_456

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cytochrome o terminal oxidase complex is the component of the aerobic respiratory chain that predominates when cells are grown at high aeration By similarity.
Catalytic activity	Ubiquinol-8 + O2 = Ubiquinone-8 + H2O.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome bo3 ubiquinol oxidase activity Inferred from electronic annotation. Source: InterPro cytochrome o ubiquinol oxidase activity Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 290	266	Ubiquinol oxidase subunit 2		PRO_0000006073
Regions
	Topological domain	25 – 42	18	Extracellular Potential
	Transmembrane	43 – 63	21	Helical; Potential
	Topological domain	64 – 87	24	Cytoplasmic Potential
	Transmembrane	88 – 108	21	Helical; Potential
	Topological domain	109 – 290	182	Extracellular Potential
Amino acid modifications
	Lipidation	25	1	N-palmitoyl cysteine Potential
	Lipidation	25	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K993 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 3D80A02A84732963	FASTA	290	33,730
	10 20 30 40 50 60 MISINFNNFF KTLLLILIAF TLHGCDSILF NPHGIIAIQE CSILLISFLI MLFVIIPVIF 70 80 90 100 110 120 MTIYFSVKYR ASNINAKYKP DWCDSKKIEI IVWTIPISII LFLAFVTWNY SHILDPKKSI 130 140 150 160 170 180 ISKYKPIKID VVSLDWRWLF IYPEYHIATI NEIMFPINRS IIFHITSNSV MNSFFIPSLG 190 200 210 220 230 240 SQIYAMPGMM TTLNLMSNSP GKYKGISSNY SGKGFSNMKF TAISVLNIKD FENWIKKAQQ 250 260 270 280 290 SPKKLNKMSI FNIISLPNEN HFIEYFSDVK KNLFYEIINQ TYSKNKVFKH

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67999.1.
RefSeq	NP_660788.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K993.
SMR	Q8K993. Positions 125-281.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg456.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67999; AAM67999; BUsg_456.
GeneID	1005709.
KEGG	bas:BUsg456.
PATRIC	21247766. VBIBucAph100086_0482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1622.
KO	K02297.
OMA	WKSTHEL.
ProtClustDB	CLSK316278.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-501-MONOMER.
Family and domain databases
Gene3D	1.10.287.90. 1 hit. 2.60.40.420. 1 hit.
InterPro	IPR012141. Bo-type_Ubol_Oxase_su_II. IPR010514. COX_ARM. IPR008972. Cupredoxin. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. IPR006333. Cyt_o_ubiquinol_oxidase_su2. [Graphical view]
Pfam	PF00116. COX2. 1 hit. PF06481. COX_ARM. 1 hit. [Graphical view]
PIRSF	PIRSF000292. Ubi_od_II. 1 hit.
SUPFAM	SSF49503. Cupredoxin. 1 hit. SSF81464. Cyt_c_oxidase_II-like_TM. 1 hit.
TIGRFAMs	TIGR01433. CyoA. 1 hit.
PROSITE	PS50857. COX2_CUA. 1 hit. PS50999. COX2_TM. 1 hit. PS51257. PROKAR_LIPOPROTEIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYOA_BUCAP

Accession

Primary (citable) accession number: Q8K993

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program