Reviewed, UniProtKB/Swiss-Prot Q8K988 (LON_BUCAP)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:	lon
Ordered Locus Names:	BUsg_461

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Degrades the regulatory proteins rcsA and sulA. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	ATP-dependent protease La		PRO_0000076126
Regions
	Domain	10 – 202	193	Lon
	Nucleotide binding	356 – 363	8	ATP Potential
Sites
	Active site	679	1	By similarity
	Active site	722	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K988-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 768ACD8B06713DD2	FASTA	777	87,755
	10 20 30 40 50 60 MNSERSERIK IPVLPLRDVV VYPHMVIPLF VGRKKSIHCI ETSMNNDKKI MLIAQKEASK 70 80 90 100 110 120 DEPSTNDLFN IGTISSILQM LKLPDGTVKV LVEGLQRACI KNIESNGEHL VAEVELIISP 130 140 150 160 170 180 TVIDKEQEVL IRTTVNQFES YIKLNKKIPS EILNTLSQTK NAEKLADTIA AHMPLKLADK 190 200 210 220 230 240 QSVLEIYNVN ERLEFLMAIM ETEIDLLKVE KRIRNRVKKQ MEKSQREYYL NEQIKAIQKE 250 260 270 280 290 300 LGDMDEIPDE NKILKRKIKS LKMPKEAKEK TESELQKLKM MSPMSAEATV VRSYIDWMIQ 310 320 330 340 350 360 VPWNIKTKIK KDIQEAKKIL DIDHFGLEKV KERILEYLAV QSRTNKVKGP ILCLIGPPGV 370 380 390 400 410 420 GKTSLGKSIA KSTGRKYVRM ALGGIRDEAE IRGHRRTYIG SMPGKLMQKM VKAKVKNPLF 430 440 450 460 470 480 LLDEIDKMSC DIRVDPASAL LEVLDPEQNI NFNDHYLEVD YDLSDVMFVA TSNSMNIPAP 490 500 510 520 530 540 LLDRMEIIRL SGYTENEKLN IAKCYLYPKQ MERNALKRNE LIITDCAIIS IIQYYTREAG 550 560 570 580 590 600 VRSLEREISK ICRKVVKLLI LNKSLKKIEI NSKNLKKFLG IKRFDYGKTN NLNQIGQVVG 610 620 630 640 650 660 LAWTEVGGEL LTIETACVSG KGKLTYTGSL GEVMQESIQA ALTVVRSQAK KLGIKKDFHE 670 680 690 700 710 720 KHDIHVHVPE GATPKDGPSA GIAMCTAIVS SLTKNPVKSN IAMTGEITLQ GRVLTIGGLK 730 740 750 760 770 EKLLAAHRGG VKTVLIPYEN KRNLEEIPKN IIEGLTIYPV KNIEEVLKIA LENTPYV

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM68004.1.
RefSeq	NP_660793.1.
3D structure databases
SMR	Q8K988. Positions 8-117, 491-584, 594-775.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Genome annotation databases
GeneID	1005704.
GenomeReviews	Gene locus BUsg_461 in contig AE013218_GR.
KEGG	bas:BUsg461.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K988.
OMA	Q8K988. VMKESIQ.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG461-MON.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_BUCAP

Accession

Primary (citable) accession number: Q8K988

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)