Q8K986 (QUEC_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
7-cyano-7-deazaguanine synthase
EC=6.3.4.20

Alternative name(s):
7-cyano-7-carbaguanine synthase
PreQ(0) synthase
Queuosine biosynthesis protein QueC

Gene names

Name:	queC
Ordered Locus Names:	BUsg_463

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ0) By similarity. HAMAP-Rule MF_01633
Catalytic activity	7-carboxy-7-carbaguanine + NH3 + ATP = 7-cyano-7-carbaguanine + ADP + phosphate + H2O. HAMAP-Rule MF_01633
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. HAMAP-Rule MF_01633
Sequence similarities	Belongs to the QueC family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	7-cyano-7-deazaguanine synthase HAMAP-Rule MF_01633		PRO_0000216267
Regions
	Nucleotide binding	8 – 18	11	ATP By similarity
Sites
	Metal binding	188	1	Zinc By similarity
	Metal binding	197	1	Zinc By similarity
	Metal binding	200	1	Zinc By similarity
	Metal binding	203	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K986 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D4C81BC737A181DC	FASTA	232	26,720
	10 20 30 40 50 60 MKKILIIFSG GQDSTTCLIH YTNIYKEIYC ITFDYNQLHK SEIDSARFIS NYFNVKKHIF 70 80 90 100 110 120 VDLKCLKNLS ISSLTDEKIS ILDNHPLNFS LPSTFVPGRN ILFLTLSSIY AFNHQINSIV 130 140 150 160 170 180 LGVNEIDFSG YPDCRNAFLK KMNDVVQIGM NCKINFQSPL INLSKAEIWA LSDYWNSTQF 190 200 210 220 230 ILNNTVTCYQ GIQGKGCGQC QSCILRNDGF NKWKSNPSYY MKKLKEKFNF DN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM68006.1.
RefSeq	NP_660795.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K986.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg463.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM68006; AAM68006; BUsg_463.
GeneID	1005702.
KEGG	bas:BUsg463.
PATRIC	21247782. VBIBucAph100086_0490.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0603.
KO	K06920.
OMA	AWGYGCG.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-508-MONOMER.
UniPathway	UPA00391.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01633. QueC.
InterPro	IPR018317. QueC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF06508. ExsB. 1 hit. [Graphical view]
PIRSF	PIRSF006293. ExsB. 1 hit.
TIGRFAMs	TIGR00364. TIGR00364. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEC_BUCAP

Accession

Primary (citable) accession number: Q8K986

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program