Q8K935 (RF3_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 3
Short name=RF-3

Gene names

Name:	prfC
Ordered Locus Names:	BUsg_523

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP By similarity. HAMAP-Rule MF_00072
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. PrfC subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC regulation of translational termination Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Peptide chain release factor 3 HAMAP-Rule MF_00072		PRO_0000210934
Regions
	Nucleotide binding	20 – 27	8	GTP By similarity
	Nucleotide binding	88 – 92	5	GTP By similarity
	Nucleotide binding	142 – 145	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K935 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B13984DD4305833B	FASTA	529	60,738
	10 20 30 40 50 60 MFNINHQLEL SKRRTFAIIS HPDAGKTTVT EKMLLLGKVI RTSGTIKARG NGKYAKSDWM 70 80 90 100 110 120 NIEKKRGISI TTSVMQFTYK NTLMNLLDTP GHEDFSEDTY RILTAVDCCL LIIDAAKGIE 130 140 150 160 170 180 DRTKKLINVS RLHNTPVITF INKLDRDSRE AIEILDEIEK ELSLDCIPIT WPISCGKNFK 190 200 210 220 230 240 GICHIHDKIV NLYQKNIVKK INFNSFSSFL NEHSLKEYLG ADLYTQLHEE LKLAAYVYPK 250 260 270 280 290 300 FSKKKFLKGD ITPVLFGSAL NNFGIDHILE SLIKWAPSPI YRVTKTRKVE PEEKKFTGFV 310 320 330 340 350 360 FKIQANMDLK HRDRIAFMRI VSGKYKKGIK LRHVRMKKDI IVSDAFSFLA GDRFSIEHAY 370 380 390 400 410 420 PGDVIGIHNH GTIKIGDTFT EGEEIKFVGI PSFAPEVFRR IFLKNPLQQK KLKKGLSQLS 430 440 450 460 470 480 EEGAIQVFRP MINNSLILGA IGILQFDVVI ERLKIEYKID AIYERVNISL ARWIRSKNKK 490 500 510 520 IISDFINKNK SYLALDISNQ LIYLAPNKAN LAVIKNIYNN IFFEKTREQ

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM68066.1.
RefSeq	NP_660855.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K935.
SMR	Q8K935. Positions 9-529.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg523.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM68066; AAM68066; BUsg_523.
GeneID	1005652.
KEGG	bas:BUsg523.
PATRIC	21247912. VBIBucAph100086_0550.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4108.
KO	K02837.
OMA	SDFMEIE.
ProtClustDB	PRK00741.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-568-MONOMER.
Family and domain databases
HAMAP	MF_00072. Rel_fac_3.
InterPro	IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR004548. PrfC. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF54980. EFG_III_V. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00503. prfC. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF3_BUCAP

Accession

Primary (citable) accession number: Q8K935

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program