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Q8K935 (RF3_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide chain release factor 3

Short name=RF-3
Gene names
Name:prfC
Ordered Locus Names:BUsg_523
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP By similarity. HAMAP-Rule MF_00072

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00072.

Sequence similarities

Belongs to the GTP-binding elongation factor family. PrfC subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processregulation of translational termination

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: InterPro

translation release factor activity, codon specific

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 529529Peptide chain release factor 3 HAMAP-Rule MF_00072
PRO_0000210934

Regions

Nucleotide binding20 – 278GTP By similarity
Nucleotide binding88 – 925GTP By similarity
Nucleotide binding142 – 1454GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K935 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: B13984DD4305833B

FASTA52960,738
        10         20         30         40         50         60 
MFNINHQLEL SKRRTFAIIS HPDAGKTTVT EKMLLLGKVI RTSGTIKARG NGKYAKSDWM 

        70         80         90        100        110        120 
NIEKKRGISI TTSVMQFTYK NTLMNLLDTP GHEDFSEDTY RILTAVDCCL LIIDAAKGIE 

       130        140        150        160        170        180 
DRTKKLINVS RLHNTPVITF INKLDRDSRE AIEILDEIEK ELSLDCIPIT WPISCGKNFK 

       190        200        210        220        230        240 
GICHIHDKIV NLYQKNIVKK INFNSFSSFL NEHSLKEYLG ADLYTQLHEE LKLAAYVYPK 

       250        260        270        280        290        300 
FSKKKFLKGD ITPVLFGSAL NNFGIDHILE SLIKWAPSPI YRVTKTRKVE PEEKKFTGFV 

       310        320        330        340        350        360 
FKIQANMDLK HRDRIAFMRI VSGKYKKGIK LRHVRMKKDI IVSDAFSFLA GDRFSIEHAY 

       370        380        390        400        410        420 
PGDVIGIHNH GTIKIGDTFT EGEEIKFVGI PSFAPEVFRR IFLKNPLQQK KLKKGLSQLS 

       430        440        450        460        470        480 
EEGAIQVFRP MINNSLILGA IGILQFDVVI ERLKIEYKID AIYERVNISL ARWIRSKNKK 

       490        500        510        520 
IISDFINKNK SYLALDISNQ LIYLAPNKAN LAVIKNIYNN IFFEKTREQ 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM68066.1.
RefSeqNP_660855.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K935.
SMRQ8K935. Positions 9-529.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg523.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM68066; AAM68066; BUsg_523.
GeneID1005652.
KEGGbas:BUsg523.
PATRIC21247912. VBIBucAph100086_0550.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4108.
KOK02837.
OMASDFMEIE.
OrthoDBEOG6677Q1.
ProtClustDBPRK00741.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-552-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00072. Rel_fac_3.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR027417. P-loop_NTPase.
IPR004548. PrfC.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 1 hit.
TIGRFAMsTIGR00503. prfC. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRF3_BUCAP
AccessionPrimary (citable) accession number: Q8K935
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names