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Q8K931 (GSHB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:BUsg_529
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutathione synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197460

Regions

Domain130 – 315186ATP-grasp
Nucleotide binding156 – 21257ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K931 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 20D4A253EEBBD83E

FASTA32037,166
        10         20         30         40         50         60 
MRKKKNLKIG IVMDSITLIN IKKDSSFAIL LEAQKRQHEI YYMEMNDLYL RKGQSYATTK 

        70         80         90        100        110        120 
SIEIQKNQNN YFKFIQKKDI SLNELDAILM RKDPPFNTEF IYATYILERA EEKGVLVINK 

       130        140        150        160        170        180 
PKSLRDCNEK IFISWFSRFT TDTLVTRKLS KIHNFWKEKN DIILKPLDAM GGKGVFRIKK 

       190        200        210        220        230        240 
DDPNFSVIVE TLTNYEKKYC MIQTYLPEVQ FGDKRILIVN GKPIPWSLTR IPKHGETRAN 

       250        260        270        280        290        300 
LAVGGEGRVQ KLNDKDWEIA NYLAPILKKR GLILVGLDVI GDKLTEINVT SPTCICEIEE 

       310        320 
KKNISITGIL IDYIEDKIYK 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM68070.1.
RefSeqNP_660859.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K931.
SMRQ8K931. Positions 7-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg529.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM68070; AAM68070; BUsg_529.
GeneID1005649.
KEGGbas:BUsg529.
PATRIC21247930. VBIBucAph100086_0559.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
KOK01920.
OMADWKIARA.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-558-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_BUCAP
AccessionPrimary (citable) accession number: Q8K931
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names