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Protein

Glutathione synthetase

Gene

gshB

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gshA)
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi286Magnesium or manganeseUniRule annotation1
Metal bindingi288Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi156 – 212ATPUniRule annotationAdd BLAST57

GO - Molecular functioni

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-561-MONOMER
UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:BUsg_529
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001974601 – 320Glutathione synthetaseAdd BLAST320

Proteomic databases

PRIDEiQ8K931

Interactioni

Protein-protein interaction databases

STRINGi198804.BUsg529

Structurei

3D structure databases

ProteinModelPortaliQ8K931
SMRiQ8K931
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini130 – 315ATP-graspUniRule annotationAdd BLAST186

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D7Z Bacteria
COG0189 LUCA
KOiK01920
OMAiWMRKDPP
OrthoDBiPOG091H04J6

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00162 GSH_S, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006284 Glut_synth_pro
IPR004218 GSHS_ATP-bd
IPR004215 GSHS_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR21621:SF4 PTHR21621:SF4, 1 hit
PfamiView protein in Pfam
PF02955 GSH-S_ATP, 1 hit
PF02951 GSH-S_N, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01380 glut_syn, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKKKNLKIG IVMDSITLIN IKKDSSFAIL LEAQKRQHEI YYMEMNDLYL
60 70 80 90 100
RKGQSYATTK SIEIQKNQNN YFKFIQKKDI SLNELDAILM RKDPPFNTEF
110 120 130 140 150
IYATYILERA EEKGVLVINK PKSLRDCNEK IFISWFSRFT TDTLVTRKLS
160 170 180 190 200
KIHNFWKEKN DIILKPLDAM GGKGVFRIKK DDPNFSVIVE TLTNYEKKYC
210 220 230 240 250
MIQTYLPEVQ FGDKRILIVN GKPIPWSLTR IPKHGETRAN LAVGGEGRVQ
260 270 280 290 300
KLNDKDWEIA NYLAPILKKR GLILVGLDVI GDKLTEINVT SPTCICEIEE
310 320
KKNISITGIL IDYIEDKIYK
Length:320
Mass (Da):37,166
Last modified:October 1, 2002 - v1
Checksum:i20D4A253EEBBD83E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA Translation: AAM68070.1
RefSeqiWP_011054036.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM68070; AAM68070; BUsg_529
KEGGibas:BUsg_529

Similar proteinsi

Entry informationi

Entry nameiGSHB_BUCAP
AccessioniPrimary (citable) accession number: Q8K931
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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