Q8K928 (Y532_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oxygen-independent coproporphyrinogen-III oxidase-like protein BUsg_532
EC=1.3.99.-

Gene names

Ordered Locus Names:

BUsg_532

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Potential.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Oxygen-independent coproporphyrinogen-III oxidase-like protein BUsg_532		PRO_0000109959
Regions
	Region	67 – 68	2	S-adenosyl-L-methionine 2 binding By similarity
Sites
	Metal binding	16	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	20	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	23	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Binding site	10	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	22	1	S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	99	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	126	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	138	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	162	1	S-adenosyl-L-methionine 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K928 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 4808E9524404C0F6	FASTA	376	44,581
	10 20 30 40 50 60 MFKLPPISLY IHIPWCIKKC GYCDFYSYVN KSFIPEKEYI DHLLKDLEKD LSLIKEREIN 70 80 90 100 110 120 SIFIGGGTPS LLKSSSIKKM MREIKKRINI SNTAEITIEA NPTTLEYKRF FNYKKSGINR 130 140 150 160 170 180 FSIGVQTFNS DLLKKIERTY NKREAILAVE EIKKINKNFN LDIMYGLPNQ SLKDVLLDLQ 190 200 210 220 230 240 YAVKYNPTHI SWYQLTLEPN TPFYVKKLNL PNENNIFKML VEGEKFLKQS GYKKYEISSY 250 260 270 280 290 300 AKLNYECQHN LNYWNFGDYI GIGCSAHGKI TQINGDIIRT IKNKNINDFM NGKYLKHKNF 310 320 330 340 350 360 VLKKDKPFEY FMNIFRLYKP VLKRQFEERT NINQNYIKEK IKKAIEKGYL KNKIDFWDTT 370 KKGKMFLNSL LKIFLD

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM68073.1.
RefSeq	NP_660862.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K928.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg532.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM68073; AAM68073; BUsg_532.
GeneID	1005648.
KEGG	bas:BUsg532.
PATRIC	21247936. VBIBucAph100086_0562.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0635.
KO	K02495.
OMA	CEHNLNY.
ProtClustDB	CLSK552878.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-575-MONOMER.
Family and domain databases
Gene3D	3.80.30.20. 1 hit.
InterPro	IPR004559. Coprogen_oxidase_HemN-rel. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. IPR023404. rSAM_horseshoe. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00539. hemN_rel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Y532_BUCAP

Accession

Primary (citable) accession number: Q8K928

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program