ID   TRMB_BUCAP              Reviewed;         239 AA.
AC   Q8K927;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 41.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
DE            EC=2.1.1.33;
DE   AltName: Full=tRNA(m7G46)-methyltransferase;
GN   Name=trmB; OrderedLocusNames=BUsg_533;
OS   Buchnera aphidicola subsp. Schizaphis graminum.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Buchnera.
OX   NCBI_TaxID=98794;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at
CC       position 46 (m7G46) in tRNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(7)-methylguanine.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. TrmB
CC       family.
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DR   EMBL; AE013218; AAM68074.1; -; Genomic_DNA.
DR   RefSeq; NP_660863.1; -.
DR   GeneID; 1005542; -.
DR   GenomeReviews; AE013218_GR; BUsg_533.
DR   KEGG; bas:BUsg533; -.
DR   HOGENOM; Q8K927; -.
DR   OMA; Q8K927; HELEGYH.
DR   BioCyc; BAPH198804:BUSG533-MON; -.
DR   GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_01057; -; 1.
DR   InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase.
DR   PANTHER; PTHR23417:SF1; Methyltransf_4; 1.
DR   Pfam; PF02390; Methyltransf_4; 1.
DR   TIGRFAMs; TIGR00091; CHP91; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    239       tRNA (guanine-N(7)-)-methyltransferase.
FT                                /FTId=PRO_0000171308.
FT   REGION      217    220       Substrate binding (Potential).
FT   BINDING      69     69       S-adenosyl-L-methionine (By similarity).
FT   BINDING      94     94       S-adenosyl-L-methionine (By similarity).
FT   BINDING     121    121       S-adenosyl-L-methionine (By similarity).
FT   BINDING     144    144       S-adenosyl-L-methionine (By similarity).
FT   BINDING     148    148       Substrate (By similarity).
FT   BINDING     180    180       Substrate (Potential).
SQ   SEQUENCE   239 AA;  27959 MW;  7F8ABFEA7E9A682B CRC64;
     MKNNIITPQY NCNGTFLRKN RSFVCRKGRI TKSQLQAIEK YWLSIGIDFK LEPLDFTSVF
     KNSAPVILEI GFGSGESLVK TAANFPNKNF LGIEVYKSGI GSCLRLAHYS GINNLKIIYH
     DAIEVIDQMI LDHTLSKVQI FFPDPWNKKR HHKRRMIQDF FLIKILKKLN NDGILHIVTD
     SKEYNFFILN LIQNIDNYIN LSKKRMCFEH FKYRLVTNFE KKAKLSGNKI FDLIFKLKK
//