Reviewed, UniProtKB/Swiss-Prot Q8K927 (TRMB_BUCAP)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	BUsg_533

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000171308
Regions
	Region	217 – 220	4	Substrate binding Potential
Sites
	Binding site	69	1	S-adenosyl-L-methionine By similarity
	Binding site	94	1	S-adenosyl-L-methionine By similarity
	Binding site	121	1	S-adenosyl-L-methionine By similarity
	Binding site	144	1	S-adenosyl-L-methionine By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	180	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K927-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7F8ABFEA7E9A682B	FASTA	239	27,959
	10 20 30 40 50 60 MKNNIITPQY NCNGTFLRKN RSFVCRKGRI TKSQLQAIEK YWLSIGIDFK LEPLDFTSVF 70 80 90 100 110 120 KNSAPVILEI GFGSGESLVK TAANFPNKNF LGIEVYKSGI GSCLRLAHYS GINNLKIIYH 130 140 150 160 170 180 DAIEVIDQMI LDHTLSKVQI FFPDPWNKKR HHKRRMIQDF FLIKILKKLN NDGILHIVTD 190 200 210 220 230 SKEYNFFILN LIQNIDNYIN LSKKRMCFEH FKYRLVTNFE KKAKLSGNKI FDLIFKLKK

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM68074.1.
RefSeq	NP_660863.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1005542.
GenomeReviews	Gene locus BUsg_533 in contig AE013218_GR.
KEGG	bas:BUsg533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K927.
OMA	Q8K927. HELEGYH.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG533-MON.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_BUCAP

Accession

Primary (citable) accession number: Q8K927

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)