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Q8K927 (TRMB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BUsg_533
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 239239tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171308

Regions

Region217 – 2204Substrate binding By similarity

Sites

Active site1441 By similarity
Binding site691S-adenosyl-L-methionine By similarity
Binding site941S-adenosyl-L-methionine By similarity
Binding site1211S-adenosyl-L-methionine By similarity
Binding site1441S-adenosyl-L-methionine By similarity
Binding site1481Substrate By similarity
Binding site1801Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8K927 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7F8ABFEA7E9A682B

FASTA23927,959
        10         20         30         40         50         60 
MKNNIITPQY NCNGTFLRKN RSFVCRKGRI TKSQLQAIEK YWLSIGIDFK LEPLDFTSVF 

        70         80         90        100        110        120 
KNSAPVILEI GFGSGESLVK TAANFPNKNF LGIEVYKSGI GSCLRLAHYS GINNLKIIYH 

       130        140        150        160        170        180 
DAIEVIDQMI LDHTLSKVQI FFPDPWNKKR HHKRRMIQDF FLIKILKKLN NDGILHIVTD 

       190        200        210        220        230 
SKEYNFFILN LIQNIDNYIN LSKKRMCFEH FKYRLVTNFE KKAKLSGNKI FDLIFKLKK 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. Translation: AAM68074.1.
RefSeqNP_660863.1. NC_004061.1.

3D structure databases

ProteinModelPortalQ8K927.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING198804.BUsg533.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM68074; AAM68074; BUsg_533.
GeneID1005542.
KEGGbas:BUsg533.
PATRIC21247938. VBIBucAph100086_0563.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAIAEVWTF.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycBAPH198804:GHMG-562-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BUCAP
AccessionPrimary (citable) accession number: Q8K927
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names