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Protein

Adenine DNA glycosylase

Gene

mutY

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine: 7,8-dihydro-8-oxoguanine (8-oxo-dGTP).By similarity

Catalytic activityi

Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei37Proton donor/acceptorBy similarity1
Sitei138Transition state stabilizerBy similarity1
Metal bindingi192Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi199Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi202Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi208Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-566-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine DNA glycosylase (EC:3.2.2.31By similarity)
Gene namesi
Name:mutY
Ordered Locus Names:BUsg_534
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022321 – 347Adenine DNA glycosylaseAdd BLAST347

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi198804.BUsg534

Structurei

3D structure databases

ProteinModelPortaliQ8K926
SMRiQ8K926
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Nth/MutY family.Curated

Phylogenomic databases

eggNOGiENOG4105CEN Bacteria
COG1194 LUCA
KOiK03575
OMAiRDPYRVW
OrthoDBiPOG091H01HV

Family and domain databases

CDDicd03431 DNA_Glycosylase_C, 1 hit
cd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 2 hits
InterProiView protein in InterPro
IPR005760 A/G_AdeGlyc_MutY
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR029119 MutY_C
IPR015797 NUDIX_hydrolase-like_dom_sf
PfamiView protein in Pfam
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
PF14815 NUDIX_4, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
SSF55811 SSF55811, 1 hit
TIGRFAMsiTIGR01084 mutY, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8K926-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIYVFSQLI LNWYHINGRK NLPWKKDKTL YKVWISEIML QQTTVKTAIP
60 70 80 90 100
YFKNFISRFP NIQSLNQSKL DDILCLWSGL GYYKRAENIY KTVKIIKEEF
110 120 130 140 150
QEKFPTGFSD LIKLPGIGRS TAGAILSLSL DYFFPILEGN VKRILMRYYG
160 170 180 190 200
IIGYVTEKKI EQKLWYLIEL ITPIHNTGSF NQGIMDIGAL ICTPKNPKCN
210 220 230 240 250
LCPLIQKCIA YKEKNWIKYP LKKKKKIILE KKSWFVVIKY QNQFWIEKNT
260 270 280 290 300
EKKIWKNLFC FPNFDTKIKT IEWLKKNKIN IDKKHKKIQS FYHKFSHFTL
310 320 330 340
HIIPILVNLS FFKNFQNSKK TGIWYDLKNT HDIGLPKPVQ KILEIFK
Length:347
Mass (Da):41,301
Last modified:October 1, 2002 - v1
Checksum:iD6F6279175293587
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013218 Genomic DNA Translation: AAM68075.1
RefSeqiWP_011054041.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM68075; AAM68075; BUsg_534
KEGGibas:BUsg_534

Similar proteinsi

Entry informationi

Entry nameiMUTY_BUCAP
AccessioniPrimary (citable) accession number: Q8K926
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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