Q8K926 (MUTY_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
A/G-specific adenine glycosylase
EC=3.2.2.-

Gene names

Name:	mutY
Ordered Locus Names:	BUsg_534

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adenine glycosylase active on G-A and C-A mispairs By similarity.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but is probably involved in the proper positioning of the enzyme along the DNA strand By similarity.
Sequence similarities	Belongs to the Nth/MutY family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW DNA N-glycosylase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	A/G-specific adenine glycosylase		PRO_0000102232
Sites
	Metal binding	192	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	199	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	202	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	208	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K926 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D6F6279175293587	FASTA	347	41,301
	10 20 30 40 50 60 MTIYVFSQLI LNWYHINGRK NLPWKKDKTL YKVWISEIML QQTTVKTAIP YFKNFISRFP 70 80 90 100 110 120 NIQSLNQSKL DDILCLWSGL GYYKRAENIY KTVKIIKEEF QEKFPTGFSD LIKLPGIGRS 130 140 150 160 170 180 TAGAILSLSL DYFFPILEGN VKRILMRYYG IIGYVTEKKI EQKLWYLIEL ITPIHNTGSF 190 200 210 220 230 240 NQGIMDIGAL ICTPKNPKCN LCPLIQKCIA YKEKNWIKYP LKKKKKIILE KKSWFVVIKY 250 260 270 280 290 300 QNQFWIEKNT EKKIWKNLFC FPNFDTKIKT IEWLKKNKIN IDKKHKKIQS FYHKFSHFTL 310 320 330 340 HIIPILVNLS FFKNFQNSKK TGIWYDLKNT HDIGLPKPVQ KILEIFK

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM68075.1.
RefSeq	NP_660864.1. NC_004061.1.
3D structure databases
ProteinModelPortal	Q8K926.
SMR	Q8K926. Positions 1-223.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg534.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM68075; AAM68075; BUsg_534.
GeneID	1005544.
KEGG	bas:BUsg534.
PATRIC	21247940. VBIBucAph100086_0564.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1194.
KO	K03575.
OMA	AIMEFGA.
ProtClustDB	CLSK493050.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-577-MONOMER.
Family and domain databases
Gene3D	1.10.1670.10. 1 hit. 1.10.340.30. 1 hit. 3.90.79.10. 1 hit.
InterPro	IPR011257. DNA_glycosylase. IPR004036. Endonuclease-III_CS2. IPR004035. Endouclease-III_FeS-bd_BS. IPR003651. Endouclease3_FeS-loop_motif. IPR003265. HhH-GPD_domain. IPR000445. HhH_motif. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR023170. HTH_base_excis_C. IPR005760. MutY_bac. IPR015797. NUDIX_hydrolase_dom-like. [Graphical view]
Pfam	PF00633. HHH. 1 hit. PF00730. HhH-GPD. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. SM00525. FES. 1 hit. SM00278. HhH1. 1 hit. [Graphical view]
SUPFAM	SSF48150. DNA_glycsylse. 1 hit. SSF55811. NUDIX_hydrolase. 1 hit.
TIGRFAMs	TIGR01084. mutY. 1 hit.
PROSITE	PS00764. ENDONUCLEASE_III_1. 1 hit. PS01155. ENDONUCLEASE_III_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MUTY_BUCAP

Accession

Primary (citable) accession number: Q8K926

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program