Reviewed, UniProtKB/Swiss-Prot Q8K911 (PTM3C_BUCAP)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICBA component
Alternative name(s):
    EIICBA-Mtl
      Short name=EII-Mtl
Including the following 3 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component
    3- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system, mannitol-specific EIIA component

Gene names

Name:	mtlA
Ordered Locus Names:	BUsg_552

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Post-translational modification	An intramolecular phosphotransfer takes places between His-563 and Cys-386 By similarity.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	PTS system mannitol-specific EIICBA component		PRO_0000186612
Regions
	Transmembrane	20 – 40	21	Potential
	Transmembrane	47 – 67	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	134 – 154	21	Potential
	Transmembrane	161 – 181	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	245 – 265	21	Potential
	Transmembrane	267 – 287	21	Potential
	Transmembrane	291 – 311	21	Potential
	Transmembrane	316 – 336	21	Potential
	Domain	12 – 340	329	PTS EIIC type-2
	Domain	380 – 472	93	PTS EIIB type-2
	Domain	503 – 645	143	PTS EIIA type-2
Sites
	Active site	386	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	563	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K911-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: B365CE4719C986C0	FASTA	649	72,358
	10 20 30 40 50 60 MFRLITLKIQ NFGQFLSNMI MPNISIFITW GIMNSLFLSL DWQYNKIFAQ LISPIIFYLL 70 80 90 100 110 120 PILIGYTGGS LIAGQRGGLL GSITTIGMIT STDMPMFLGG MVAGPLGGWI IKGFDQIIKN 130 140 150 160 170 180 RIKSGFEMLV NNFSIAIIGI FLTIFSFFFI GPFIEGVSKF LGCLIKIIID FNLLPLIAFI 190 200 210 220 230 240 IEPAKVFFLN NAINHGIFSP LGIQEISENK SSLFFLIESN PGPGLGILIA CFFFGKEKSY 250 260 270 280 290 300 KSSGTAAIVQ FLGGIHEIYF SYVLIKPILM ISLILGSMTS IFMLVFFKGG LIAAVSPGSI 310 320 330 340 350 360 LSILAMTKKG LYFANLISIF SSFLISFLSA MLFFKINLGQ KIKNDKSSIQ LFKNNLFPIK 370 380 390 400 410 420 KIGDEKYDFI KKIKNFQKIK NIIIACDAGM GSSAMGASIL RKKIKSNNLT HIYVSNIAIN 430 440 450 460 470 480 LLPKNADLVI THEKLTYLAK KRAPDAQHIS LTNFLDSNFY TSLVKQLDLN KDFFKKNNIQ 490 500 510 520 530 540 DKKDRITQIN IDSHKEIQSK CFFQISDKNI FLNQYAENKK EAIKIVGKHL VAQGYVKKDY 550 560 570 580 590 600 IDAMLDREKI ASTWLGESVA LPHGTIKSKD SILKTGVIFC QFPKGVHFGE TIDDIAYLVI 610 620 630 640 GIAAKNNEHI MVVSNITNAL DDKDVISKLS KTKSVKEVLS YLNVNVKKN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. Translation: AAM68090.1.
RefSeq	NP_660879.1.
3D structure databases
HSSP	HSSP built from PDB template 1A3A based on UniProtKB P00550.
ModBase	Search...
Genome annotation databases
GeneID	1005638.
GenomeReviews	Gene locus BUsg_552 in contig AE013218_GR.
KEGG	bas:BUsg552.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K911.
OMA	Q8K911. ITHKDLT.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG552-MON.
Family and domain databases
InterPro	IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003501. PTS_IIB_lac. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
ProDom	PD001689. PTS_EIIA_2. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTM3C_BUCAP

Accession

Primary (citable) accession number: Q8K911

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)