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Q8K901

- DAPF_BUCAP

UniProt

Q8K901 - DAPF_BUCAP

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211SubstrateUniRule annotation
    Binding sitei54 – 541SubstrateUniRule annotation
    Binding sitei74 – 741SubstrateUniRule annotation
    Active sitei83 – 831Proton donor/acceptorUniRule annotation
    Binding sitei167 – 1671SubstrateUniRule annotation
    Sitei169 – 1691Important for catalytic activityUniRule annotation
    Binding sitei200 – 2001SubstrateUniRule annotation
    Sitei218 – 2181Important for catalytic activityUniRule annotation
    Active sitei227 – 2271Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciBAPH198804:GHMG-600-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:BUsg_568
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284Diaminopimelate epimerasePRO_0000149826Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi83 ↔ 227

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi198804.BUsg568.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K901.
    SMRiQ8K901. Positions 11-284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni18 – 192Substrate bindingUniRule annotation
    Regioni83 – 853Substrate bindingUniRule annotation
    Regioni218 – 2192Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K901-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSNYKKKKK IYFSKMHGLG NDFMVIESIK QDFILTKSII ENLSNRNTGV    50
    GFDQLLLIEK SNNFKFDFHY RIFNANGNEV EQCGNGARCV GLFLFLKGLI 100
    NKRKIMLSTN KKNISIKFMS DNNIQVNMNE PSFKLEEISI SKKIVLNNFS 150
    VKLINKNLKC SLVSIGNPHC VIKVESINNA PVKKIGELLE KNSIFPQGIN 200
    VGFMKVINKN HIQLRVYERD VGETQSCGSG ACAAVAVGIA KNLLCDNVKV 250
    DLLGGNLIIK WKGFNYPLYM IGTACHIYDG YIYI 284
    Length:284
    Mass (Da):32,025
    Last modified:October 1, 2002 - v1
    Checksum:iEB37E370320A0089
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM68104.1.
    RefSeqiNP_660893.2. NC_004061.1.

    Genome annotation databases

    EnsemblBacteriaiAAM68104; AAM68104; BUsg_568.
    GeneIDi1005624.
    KEGGibas:BUsg568.
    PATRICi21248024. VBIBucAph100086_0603.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. Translation: AAM68104.1 .
    RefSeqi NP_660893.2. NC_004061.1.

    3D structure databases

    ProteinModelPortali Q8K901.
    SMRi Q8K901. Positions 11-284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198804.BUsg568.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM68104 ; AAM68104 ; BUsg_568 .
    GeneIDi 1005624.
    KEGGi bas:BUsg568.
    PATRICi 21248024. VBIBucAph100086_0603.

    Phylogenomic databases

    eggNOGi COG0253.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci BAPH198804:GHMG-600-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiDAPF_BUCAP
    AccessioniPrimary (citable) accession number: Q8K901
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3