Reviewed, UniProtKB/Swiss-Prot Q8K7C0 (SPX_STRP3)

Names and origin

Protein names

Recommended name:
    Regulatory protein spx

Gene names

Name:	spxA
Ordered Locus Names:	SpyM3_0885, SPs1085

Organism

Streptococcus pyogenes serotype M3 [Complete proteome] [HAMAP]

Taxonomic identifier

301448 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interferes with activator-stimulated transcription by interaction with the RNA polymerase alpha-CTD. May function to globally reduce transcription of genes involved in growth- and development-promoting processes and to increase transcription of genes involved in thiol homeostasis, during periods of extreme stress By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the arsC family. Spx subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Redox-active center
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription regulator activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Regulatory protein spx HAMAP MF_01132		PRO_0000162578
Amino acid modifications
	Disulfide bond	10 ↔ 13		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K7C0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 41549F88EC2E214E	FASTA	134	15,514
	10 20 30 40 50 60 MVTLFLSPSC TSCRKARAWL VKHEVDFQEH NIITSPLSRD ELMSILSFTE NGTEDIISTR 70 80 90 100 110 120 SKVFQKLDVD VEELSISDLI DLIAKNPSLL RRPIIMDQKR MQIGFNEDEI RAFLSRDYRK 130 QELRQATIKA EIEG

References

[1]

"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence." Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed: 12122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-595 / MGAS315 / Serotype M3.

[2]

"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed: 12799345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1 / Serotype M3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014074 Genomic DNA. Translation: AAM79492.1. BA000034 Genomic DNA. Translation: BAC64180.1.
RefSeq	NP_664689.1. NP_802347.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1009200. 1065769.
GenomeReviews	Gene locus SpyM3_0885 in contig AE014074_GR. Gene locus SPs1085 in contig BA000034_GR.
KEGG	spg:SpyM3_0885. sps:SPs1085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K7C0.
OMA	Q8K7C0. FTERNIF.
Enzyme and pathway databases
BioCyc	SPYO193567:SPS1085-MON. SPYO198466:SPYM3_0885-MON.
Family and domain databases
HAMAP	MF_01132. [Tree]
InterPro	IPR006660. Arsenate_reductase-like. IPR006504. CHP1617_ArsC_rel. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF03960. ArsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01617. arsC_related. 1 hit.
PROSITE	PS51353. ARSC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPX_STRP3

Accession

Primary (citable) accession number: Q8K7C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)