ID   GLPO_STRP3              Reviewed;         612 AA.
AC   Q8K666;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 47.
DE   RecName: Full=Alpha-glycerophosphate oxidase;
DE            EC=1.1.3.21;
DE   AltName: Full=Glycerol-3-phosphate oxidase;
GN   Name=glpO; OrderedLocusNames=SpyM3_1467, SPs0399;
OS   Streptococcus pyogenes serotype M3.
OC   Bacteria; Firmicutes; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=301448;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-595 / MGAS315 / Serotype M3;
RX   MEDLINE=22133808; PubMed=12122206; DOI=10.1073/pnas.152298499;
RA   Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S.,
RA   Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D.,
RA   Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M.,
RA   Schlievert P.M., Musser J.M.;
RT   "Genome sequence of a serotype M3 strain of group A Streptococcus:
RT   phage-encoded toxins, the high-virulence phenotype, and clone
RT   emergence.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SSI-1 / Serotype M3;
RX   MEDLINE=22683278; PubMed=12799345; DOI=10.1101/gr.1096703;
RA   Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y.,
RA   Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T.,
RA   Hayashi H., Hattori M., Hamada S.;
RT   "Genome sequence of an M3 strain of Streptococcus pyogenes reveals a
RT   large-scale genomic rearrangement in invasive strains and new insights
RT   into phage evolution.";
RL   Genome Res. 13:1042-1055(2003).
CC   -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + O(2) = glycerone
CC       phosphate + H(2)O(2).
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC       dehydrogenase family.
CC   -!- CAUTION: As S.pyogenes is unable to produce acid from glycerol,
CC       the significance and/or function of the glpO gene in this organism
CC       is at present unknown.
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DR   EMBL; AE014074; AAM80074.1; -; Genomic_DNA.
DR   EMBL; BA000034; BAC63494.1; -; Genomic_DNA.
DR   RefSeq; NP_665271.1; -.
DR   RefSeq; NP_801661.1; -.
DR   GeneID; 1009782; -.
DR   GeneID; 1066923; -.
DR   GenomeReviews; AE014074_GR; SpyM3_1467.
DR   GenomeReviews; BA000034_GR; SPs0399.
DR   KEGG; spg:SpyM3_1467; -.
DR   KEGG; sps:SPs0399; -.
DR   HOGENOM; Q8K666; -.
DR   OMA; Q8K666; KDIESSW.
DR   BioCyc; SPYO193567:SPS0399-MON; -.
DR   BioCyc; SPYO198466:SPYM3_1467-MON; -.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0004369; F:glycerol-3-phosphate oxidase activity; IEA:EC.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000447; FAD-dep_Gly3P_DH.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   Pfam; PF01266; DAO; 1.
DR   PRINTS; PR01001; FADG3PDH.
DR   PROSITE; PS00977; FAD_G3PDH_1; 1.
DR   PROSITE; PS00978; FAD_G3PDH_2; FALSE_NEG.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Glycerol metabolism;
KW   Oxidoreductase.
FT   CHAIN         1    612       Alpha-glycerophosphate oxidase.
FT                                /FTId=PRO_0000126111.
FT   NP_BIND      21     49       FAD (Potential).
SQ   SEQUENCE   612 AA;  67653 MW;  10BD47B2ED358B47 CRC64;
     MEFSRETRRL ALQKMQERDL DLLIIGGGIT GAGVALQAAA SGLDTGLIEM QDFAQGTSSR
     STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKP DPMLLPVYDE PGSTFSMFRL
     KVAMDLYDLL AGVSNTPAAN KVLTKEEVLK REPDLKQEGL LGGGVYLDFR NNDARLVIEN
     IKRANRDGAL IASHVKAEDF LLDDDGKIIG VKARDLLSDQ EIIIKAKLVI NTTGPWSDEI
     RQFSHKGQPI HQMRPTKGVH LVVDRQKLPV SQPVYVDTGL NDGRMVFVLP REEKTYFGTT
     DTDYTGDLEH PQVTQEDVDY LLGVVNNRFP NANVTIDDIE SSWAGLRPLL SGNSASDYNG
     GNSGKVSDDS FDHLVDTVKA YINHEDSREA VEKAIKQVET STSEKELDPS AVSRGSSFDR
     DENGLFTLAG GKITDYRKMA EGALTGIIQI LKEEFGKSFK LINSKTYPVS GGEINPANVD
     LEIEAYAQLG TLSGLSMDDA RYLANLYGSN APKVFALTRQ LTAAEGLSLA ETLSLHYAMD
     YEMALKPTDY FLRRTNHLLF MRDSLDALID PVINEMAKHF EWSDQERVAQ EDDLRRVIAD
     NDLSALKGHQ EG
//