Reviewed, UniProtKB/Swiss-Prot Q8K666 (GLPO_STRP3)

Names and origin

Protein names

Recommended name:
    Alpha-glycerophosphate oxidase
    EC=1.1.3.21
Alternative name(s):
    Glycerol-3-phosphate oxidase

Gene names

Name:	glpO
Ordered Locus Names:	SpyM3_1467, SPs0399

Organism

Streptococcus pyogenes serotype M3 [Complete proteome] [HAMAP]

Taxonomic identifier

301448 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
Caution	As S.pyogenes is unable to produce acid from glycerol, the significance and/or function of the glpO gene in this organism is at present unknown.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	glycerol-3-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Alpha-glycerophosphate oxidase		PRO_0000126111
Regions
	Nucleotide binding	21 – 49	29	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8K666-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 10BD47B2ED358B47	FASTA	612	67,653
	10 20 30 40 50 60 MEFSRETRRL ALQKMQERDL DLLIIGGGIT GAGVALQAAA SGLDTGLIEM QDFAQGTSSR 70 80 90 100 110 120 STKLVHGGLR YLKQFDVEVV SDTVSERAVV QQIAPHIPKP DPMLLPVYDE PGSTFSMFRL 130 140 150 160 170 180 KVAMDLYDLL AGVSNTPAAN KVLTKEEVLK REPDLKQEGL LGGGVYLDFR NNDARLVIEN 190 200 210 220 230 240 IKRANRDGAL IASHVKAEDF LLDDDGKIIG VKARDLLSDQ EIIIKAKLVI NTTGPWSDEI 250 260 270 280 290 300 RQFSHKGQPI HQMRPTKGVH LVVDRQKLPV SQPVYVDTGL NDGRMVFVLP REEKTYFGTT 310 320 330 340 350 360 DTDYTGDLEH PQVTQEDVDY LLGVVNNRFP NANVTIDDIE SSWAGLRPLL SGNSASDYNG 370 380 390 400 410 420 GNSGKVSDDS FDHLVDTVKA YINHEDSREA VEKAIKQVET STSEKELDPS AVSRGSSFDR 430 440 450 460 470 480 DENGLFTLAG GKITDYRKMA EGALTGIIQI LKEEFGKSFK LINSKTYPVS GGEINPANVD 490 500 510 520 530 540 LEIEAYAQLG TLSGLSMDDA RYLANLYGSN APKVFALTRQ LTAAEGLSLA ETLSLHYAMD 550 560 570 580 590 600 YEMALKPTDY FLRRTNHLLF MRDSLDALID PVINEMAKHF EWSDQERVAQ EDDLRRVIAD 610 NDLSALKGHQ EG

References

[1]

"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence." Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed: 12122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-595 / MGAS315 / Serotype M3.

[2]

"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed: 12799345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1 / Serotype M3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014074 Genomic DNA. Translation: AAM80074.1. BA000034 Genomic DNA. Translation: BAC63494.1.
RefSeq	NP_665271.1. NP_801661.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1009782. 1066923.
GenomeReviews	Gene locus SpyM3_1467 in contig AE014074_GR. Gene locus SPs0399 in contig BA000034_GR.
KEGG	spg:SpyM3_1467. sps:SPs0399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K666.
OMA	Q8K666. KDIESSW.
Enzyme and pathway databases
BioCyc	SPYO193567:SPS0399-MON. SPYO198466:SPYM3_1467-MON.
Family and domain databases
InterPro	IPR000447. FAD-dep_Gly3P_DH. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR01001. FADG3PDH.
PROSITE	PS00977. FAD_G3PDH_1. 1 hit. PS00978. FAD_G3PDH_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPO_STRP3

Accession

Primary (citable) accession number: Q8K666

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)