Reviewed, UniProtKB/Swiss-Prot Q8K5V1 (LACG_STRP3)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	SpyM3_1653, SPs1651

Organism

Streptococcus pyogenes serotype M3 [Complete proteome] [HAMAP]

Taxonomic identifier

301448 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase HAMAP MF_01574		PRO_0000260740
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8K5V1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 511359DDE5476CEE	FASTA	468	53,874
	10 20 30 40 50 60 MTKTLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYNRYPVDL 70 80 90 100 110 120 KLSEEFGVNG IRISIAWSRI FPTGKGEVNP KGVEYYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSDGDFL NRENIEHFVN YAEFCFKEFS EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVSHARAV KLFKDSGYSG EIGVVHALPT KYPFDANNPD DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSDKTM EGVNHILEVN GGELDLREED FAALDAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMQAFDGE TEIIHNGKGE KGSSKYQIKG VGRRKAPVDV PKTDWDWIIF PQGLYDQIMR 370 380 390 400 410 420 VKADYPNYKK IYITENGLGY KDEFVDNTVY DDGRIDYVKK HLEVISDAIS DGVNVKGYFM 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAYWYKK VAETQVIE

References

[1]

"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence." Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed: 12122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-595 / MGAS315 / Serotype M3.

[2]

"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed: 12799345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1 / Serotype M3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014074 Genomic DNA. Translation: AAM80260.1. BA000034 Genomic DNA. Translation: BAC64746.1.
RefSeq	NP_665457.1. NP_802913.1.
3D structure databases
HSSP	HSSP built from PDB template 2PBG based on UniProtKB P11546.
SMR	Q8K5V1. Positions 1-468.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
GeneID	1009968. 1066380.
GenomeReviews	Gene locus SpyM3_1653 in contig AE014074_GR. Gene locus SPs1651 in contig BA000034_GR.
KEGG	spg:SpyM3_1653. sps:SPs1651.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8K5V1.
OMA	FCFKEFS.
Enzyme and pathway databases
BioCyc	SPYO193567:SPS1651-MON. SPYO198466:SPYM3_1653-MON.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_STRP3

Accession

Primary (citable) accession number: Q8K5V1
Secondary accession number(s): Q79W99

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)