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Q8K4Z6 (GPC6A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor family C group 6 member A
Gene names
Name:Gprc6a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length928 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca2+ and Mg2+. Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G11 and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids. Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure

Homodimer; disulfide-linked. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Expressed at high level in liver, lung, spleen and heart. Expressed at lower level in kidney, skeletal muscle and brain. Expressed in E7, E11, E15 and E17 embryos. Ref.1 Ref.5

Post-translational modification

N-glycosylated. Ref.1

Disruption phenotype

Deficient mice shown normal body weight, an increased fat mass, decreased lean body, hyperglycemia and insulin resistance, proteinuria, renal calcium, phosphate wasting, impaired bone mineral density and defective testicular function. Ref.6 Ref.7

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8K4Z6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8K4Z6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     271-445: Missing.
Isoform 3 (identifier: Q8K4Z6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     446-516: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 928908G-protein coupled receptor family C group 6 member A
PRO_0000043197

Regions

Topological domain21 – 594574Extracellular Potential
Transmembrane595 – 61521Helical; Potential
Topological domain616 – 63015Cytoplasmic Potential
Transmembrane631 – 65121Helical; Potential
Topological domain652 – 66918Extracellular Potential
Transmembrane670 – 69021Helical; Potential
Topological domain691 – 70616Cytoplasmic Potential
Transmembrane707 – 72721Helical; Potential
Topological domain728 – 75023Extracellular Potential
Transmembrane751 – 77121Helical; Potential
Topological domain772 – 78413Cytoplasmic Potential
Transmembrane785 – 80521Helical; Potential
Topological domain806 – 8127Extracellular Potential
Transmembrane813 – 83321Helical; Potential
Topological domain834 – 92895Cytoplasmic Potential

Amino acid modifications

Glycosylation3321N-linked (GlcNAc...) Potential
Glycosylation5551N-linked (GlcNAc...) Potential
Glycosylation5671N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence271 – 445175Missing in isoform 2.
VSP_016456
Alternative sequence446 – 51671Missing in isoform 3.
VSP_016457

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: D670E3FEC700BD9B

FASTA928104,213
        10         20         30         40         50         60 
MALLITVVTC FMIILDTSQS CHTPDDFVAI TSPGHIMIGG LFAIHEKMLS SDDHPRRPQI 

        70         80         90        100        110        120 
QKCAGFEISV FLQTLAMIHS IEMINNSTLL SGVKLGYEIY DTCTEVTAAM AATLRFLSKF 

       130        140        150        160        170        180 
NCSRETVVFQ CDYSSYMPRV KAVIGAGYSE TSIAVSRMLN LQLMPQVSYE STAEILSDKI 

       190        200        210        220        230        240 
RFPSFLRTVP SDFYQTKAMA HLIRQSGWNW IGAITTDDDY GRLALNTFAI QAAENNVCIA 

       250        260        270        280        290        300 
FKEVLPAFLS DNTIEVRINQ TLEKIIAEAQ VNVIVVFLRK FHVFNLFTKA IERKISKIWI 

       310        320        330        340        350        360 
ASDNWSTATK IITIPNVKKL GKVVGFAFRR GNTSSFHSFL QTLHMYPNDN NKPLHEFAML 

       370        380        390        400        410        420 
VSACKYIKDG DLSQCISNYS QATLTYDTTK TIENHLFKRN DFLWHYTEPG LIYSIQLAVF 

       430        440        450        460        470        480 
ALGHAIRDLC QARDCKKPNA FQPWELLAVL KNVTFTDGRN SFHFDAHGDL NTGYDVVLWK 

       490        500        510        520        530        540 
ETNGLMTVTK MAEYDLQRDV FITTNQETKH EFRKLKQILS KCSKECSPGQ MKKATGSQHS 

       550        560        570        580        590        600 
CCYECVSCPE NHYSNETDMD HCLLCNNETH WAPVRSTTCF EKEVEYLDWD DSLALLLIAL 

       610        620        630        640        650        660 
SLLGIAFVLA IGIIFTRNLK TPVVKSSGGL VVCYVMLICH ALNFASTGFF IGEPQDFACK 

       670        680        690        700        710        720 
TRQTLFGVSF TLCVSCILTK SLKILLAFSF DPKLTMFLKC LYRPVPIVLT CTGIQVVICT 

       730        740        750        760        770        780 
LWLVLAAPSV EENISLPRVI ILECEEGSAL AFGTMLGYIT VLAFICFVFA FKGRKLPENY 

       790        800        810        820        830        840 
NEAKFLTFGM LIYFIAWITF IPVYTTTFGK YLPAVEIIVI LISNYGILCC IFFPKCYIIL 

       850        860        870        880        890        900 
CKQKTNTKSA FLQMVYNYSA HSVDSLALSH VSLDSTSYDT ATTNQSPGNK MTACQNDNHL 

       910        920 
PAQVLPHTGT AKTIKASKTL RQKRSSSI 

« Hide

Isoform 2 [UniParc].

Checksum: B9ADC85A644F2CD1
Show »

FASTA75383,999
Isoform 3 [UniParc].

Checksum: AF97BC4E8F20E7F7
Show »

FASTA85795,968

References

« Hide 'large scale' references
[1]"Cloning and characterization of a family C orphan G-protein coupled receptor."
Kuang D., Yao Y., Lam J., Tsushima R.G., Hampson D.R.
J. Neurochem. 93:383-391(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION, SUBUNIT, GLYCOSYLATION, TISSUE SPECIFICITY.
Strain: CD-1.
[2]"Cloning of a metabotropic glutamate/pheromone receptor (Gprc6a)."
Lorenz-Depiereux B., Dorner M., Strom T.M.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster / NIH.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Deorphanization of GPRC6A: a promiscuous L-alpha-amino acid receptor with preference for basic amino acids."
Wellendorph P., Hansen K.B., Balsgaard A., Greenwood J.R., Egebjerg J., Brauener-Osborne H.
Mol. Pharmacol. 67:589-597(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Identification of a novel extracellular cation-sensing G-protein-coupled receptor."
Pi M., Faber P., Ekema G., Jackson P.D., Ting A., Wang N., Fontilla-Poole M., Mays R.W., Brunden K.R., Harrington J.J., Quarles L.D.
J. Biol. Chem. 280:40201-40209(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"GPRC6A null mice exhibit osteopenia, feminization and metabolic syndrome."
Pi M., Chen L., Huang M.Z., Zhu W., Ringhofer B., Luo J., Christenson L., Li B., Zhang J., Jackson P.D., Faber P., Brunden K.R., Harrington J.J., Quarles L.D.
PLoS ONE 3:E3858-E3858(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"GPRC6A mediates the non-genomic effects of steroids."
Pi M., Parrill A.L., Quarles L.D.
J. Biol. Chem. 285:39953-39964(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY735399 mRNA. Translation: AAW68012.1.
AY735400 mRNA. Translation: AAW68013.1.
AY735401 mRNA. Translation: AAW68014.1.
AY101365 mRNA. Translation: AAM22280.1.
BC108998 mRNA. Translation: AAI08999.1.
RefSeqNP_694711.1. NM_153071.1.
UniGeneMm.221031.

3D structure databases

ProteinModelPortalQ8K4Z6.
SMRQ8K4Z6. Positions 28-580.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4997775.

Chemistry

GuidetoPHARMACOLOGY55.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ8K4Z6.

Proteomic databases

PRIDEQ8K4Z6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020062; ENSMUSP00000020062; ENSMUSG00000019905. [Q8K4Z6-1]
GeneID210198.
KEGGmmu:210198.
UCSCuc007fau.1. mouse. [Q8K4Z6-1]
uc007fav.1. mouse. [Q8K4Z6-2]
uc007faw.1. mouse. [Q8K4Z6-3]

Organism-specific databases

CTD222545.
MGIMGI:2429498. Gprc6a.

Phylogenomic databases

eggNOGNOG295353.
GeneTreeENSGT00750000117484.
HOGENOMHOG000063697.
HOVERGENHBG096042.
InParanoidQ8K4Z6.
KOK04622.
OMAETHWAPV.
OrthoDBEOG7DZ8J8.
PhylomeDBQ8K4Z6.
TreeFamTF331269.

Gene expression databases

BgeeQ8K4Z6.
GenevestigatorQ8K4Z6.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio372908.
PROQ8K4Z6.
SOURCESearch...

Entry information

Entry nameGPC6A_MOUSE
AccessionPrimary (citable) accession number: Q8K4Z6
Secondary accession number(s): Q5DK51, Q5DK52
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2002
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries