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Q8K4Z6

- GPC6A_MOUSE

UniProt

Q8K4Z6 - GPC6A_MOUSE

Protein

G-protein coupled receptor family C group 6 member A

Gene

Gprc6a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca2+ and Mg2+. Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids.4 Publications

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: MGI

    GO - Biological processi

    1. calcium-mediated signaling Source: MGI
    2. G-protein coupled receptor signaling pathway Source: MGI
    3. response to amino acid Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G-protein coupled receptor family C group 6 member A
    Gene namesi
    Name:Gprc6a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:2429498. Gprc6a.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Deficient mice shown normal body weight, an increased fat mass, decreased lean body, hyperglycemia and insulin resistance, proteinuria, renal calcium, phosphate wasting, impaired bone mineral density and defective testicular function.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 928908G-protein coupled receptor family C group 6 member APRO_0000043197Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi567 – 5671N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8K4Z6.

    PTM databases

    PhosphoSiteiQ8K4Z6.

    Expressioni

    Tissue specificityi

    Expressed at high level in liver, lung, spleen and heart. Expressed at lower level in kidney, skeletal muscle and brain. Expressed in E7, E11, E15 and E17 embryos.2 Publications

    Gene expression databases

    BgeeiQ8K4Z6.
    GenevestigatoriQ8K4Z6.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.1 Publication

    Protein-protein interaction databases

    MINTiMINT-4997775.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8K4Z6.
    SMRiQ8K4Z6. Positions 34-580, 584-846.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 594574ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini616 – 63015CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini652 – 66918ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini691 – 70616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini728 – 75023ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini772 – 78413CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini806 – 8127ExtracellularSequence Analysis
    Topological domaini834 – 92895CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei595 – 61521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei631 – 65121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei670 – 69021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei707 – 72721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei751 – 77121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei785 – 80521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei813 – 83321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295353.
    GeneTreeiENSGT00750000117484.
    HOGENOMiHOG000063697.
    HOVERGENiHBG096042.
    InParanoidiQ8K4Z6.
    KOiK04622.
    OMAiETHWAPV.
    OrthoDBiEOG7DZ8J8.
    PhylomeDBiQ8K4Z6.
    TreeFamiTF331269.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view]
    PRINTSiPR00248. GPCRMGR.
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8K4Z6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALLITVVTC FMIILDTSQS CHTPDDFVAI TSPGHIMIGG LFAIHEKMLS    50
    SDDHPRRPQI QKCAGFEISV FLQTLAMIHS IEMINNSTLL SGVKLGYEIY 100
    DTCTEVTAAM AATLRFLSKF NCSRETVVFQ CDYSSYMPRV KAVIGAGYSE 150
    TSIAVSRMLN LQLMPQVSYE STAEILSDKI RFPSFLRTVP SDFYQTKAMA 200
    HLIRQSGWNW IGAITTDDDY GRLALNTFAI QAAENNVCIA FKEVLPAFLS 250
    DNTIEVRINQ TLEKIIAEAQ VNVIVVFLRK FHVFNLFTKA IERKISKIWI 300
    ASDNWSTATK IITIPNVKKL GKVVGFAFRR GNTSSFHSFL QTLHMYPNDN 350
    NKPLHEFAML VSACKYIKDG DLSQCISNYS QATLTYDTTK TIENHLFKRN 400
    DFLWHYTEPG LIYSIQLAVF ALGHAIRDLC QARDCKKPNA FQPWELLAVL 450
    KNVTFTDGRN SFHFDAHGDL NTGYDVVLWK ETNGLMTVTK MAEYDLQRDV 500
    FITTNQETKH EFRKLKQILS KCSKECSPGQ MKKATGSQHS CCYECVSCPE 550
    NHYSNETDMD HCLLCNNETH WAPVRSTTCF EKEVEYLDWD DSLALLLIAL 600
    SLLGIAFVLA IGIIFTRNLK TPVVKSSGGL VVCYVMLICH ALNFASTGFF 650
    IGEPQDFACK TRQTLFGVSF TLCVSCILTK SLKILLAFSF DPKLTMFLKC 700
    LYRPVPIVLT CTGIQVVICT LWLVLAAPSV EENISLPRVI ILECEEGSAL 750
    AFGTMLGYIT VLAFICFVFA FKGRKLPENY NEAKFLTFGM LIYFIAWITF 800
    IPVYTTTFGK YLPAVEIIVI LISNYGILCC IFFPKCYIIL CKQKTNTKSA 850
    FLQMVYNYSA HSVDSLALSH VSLDSTSYDT ATTNQSPGNK MTACQNDNHL 900
    PAQVLPHTGT AKTIKASKTL RQKRSSSI 928
    Length:928
    Mass (Da):104,213
    Last modified:October 1, 2002 - v1
    Checksum:iD670E3FEC700BD9B
    GO
    Isoform 2 (identifier: Q8K4Z6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         271-445: Missing.

    Show »
    Length:753
    Mass (Da):83,999
    Checksum:iB9ADC85A644F2CD1
    GO
    Isoform 3 (identifier: Q8K4Z6-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         446-516: Missing.

    Show »
    Length:857
    Mass (Da):95,968
    Checksum:iAF97BC4E8F20E7F7
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei271 – 445175Missing in isoform 2. 1 PublicationVSP_016456Add
    BLAST
    Alternative sequencei446 – 51671Missing in isoform 3. 1 PublicationVSP_016457Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY735399 mRNA. Translation: AAW68012.1.
    AY735400 mRNA. Translation: AAW68013.1.
    AY735401 mRNA. Translation: AAW68014.1.
    AY101365 mRNA. Translation: AAM22280.1.
    BC108998 mRNA. Translation: AAI08999.1.
    CCDSiCCDS23834.1. [Q8K4Z6-1]
    RefSeqiNP_694711.1. NM_153071.1. [Q8K4Z6-1]
    UniGeneiMm.221031.

    Genome annotation databases

    EnsembliENSMUST00000020062; ENSMUSP00000020062; ENSMUSG00000019905. [Q8K4Z6-1]
    GeneIDi210198.
    KEGGimmu:210198.
    UCSCiuc007fau.1. mouse. [Q8K4Z6-1]
    uc007fav.1. mouse. [Q8K4Z6-2]
    uc007faw.1. mouse. [Q8K4Z6-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY735399 mRNA. Translation: AAW68012.1 .
    AY735400 mRNA. Translation: AAW68013.1 .
    AY735401 mRNA. Translation: AAW68014.1 .
    AY101365 mRNA. Translation: AAM22280.1 .
    BC108998 mRNA. Translation: AAI08999.1 .
    CCDSi CCDS23834.1. [Q8K4Z6-1 ]
    RefSeqi NP_694711.1. NM_153071.1. [Q8K4Z6-1 ]
    UniGenei Mm.221031.

    3D structure databases

    ProteinModelPortali Q8K4Z6.
    SMRi Q8K4Z6. Positions 34-580, 584-846.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4997775.

    Chemistry

    GuidetoPHARMACOLOGYi 55.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q8K4Z6.

    Proteomic databases

    PRIDEi Q8K4Z6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020062 ; ENSMUSP00000020062 ; ENSMUSG00000019905 . [Q8K4Z6-1 ]
    GeneIDi 210198.
    KEGGi mmu:210198.
    UCSCi uc007fau.1. mouse. [Q8K4Z6-1 ]
    uc007fav.1. mouse. [Q8K4Z6-2 ]
    uc007faw.1. mouse. [Q8K4Z6-3 ]

    Organism-specific databases

    CTDi 222545.
    MGIi MGI:2429498. Gprc6a.

    Phylogenomic databases

    eggNOGi NOG295353.
    GeneTreei ENSGT00750000117484.
    HOGENOMi HOG000063697.
    HOVERGENi HBG096042.
    InParanoidi Q8K4Z6.
    KOi K04622.
    OMAi ETHWAPV.
    OrthoDBi EOG7DZ8J8.
    PhylomeDBi Q8K4Z6.
    TreeFami TF331269.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    NextBioi 372908.
    PROi Q8K4Z6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K4Z6.
    Genevestigatori Q8K4Z6.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view ]
    PRINTSi PR00248. GPCRMGR.
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a family C orphan G-protein coupled receptor."
      Kuang D., Yao Y., Lam J., Tsushima R.G., Hampson D.R.
      J. Neurochem. 93:383-391(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION, SUBUNIT, GLYCOSYLATION, TISSUE SPECIFICITY.
      Strain: CD-1.
    2. "Cloning of a metabotropic glutamate/pheromone receptor (Gprc6a)."
      Lorenz-Depiereux B., Dorner M., Strom T.M.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss Webster / NIH.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Deorphanization of GPRC6A: a promiscuous L-alpha-amino acid receptor with preference for basic amino acids."
      Wellendorph P., Hansen K.B., Balsgaard A., Greenwood J.R., Egebjerg J., Brauener-Osborne H.
      Mol. Pharmacol. 67:589-597(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: FUNCTION, TISSUE SPECIFICITY.
    6. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    7. "GPRC6A mediates the non-genomic effects of steroids."
      Pi M., Parrill A.L., Quarles L.D.
      J. Biol. Chem. 285:39953-39964(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiGPC6A_MOUSE
    AccessioniPrimary (citable) accession number: Q8K4Z6
    Secondary accession number(s): Q5DK51, Q5DK52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3