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Q8K4Z6

- GPC6A_MOUSE

UniProt

Q8K4Z6 - GPC6A_MOUSE

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Protein

G-protein coupled receptor family C group 6 member A

Gene

Gprc6a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca2+ and Mg2+. Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids.4 Publications

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: MGI

GO - Biological processi

  1. calcium-mediated signaling Source: MGI
  2. G-protein coupled receptor signaling pathway Source: MGI
  3. response to amino acid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_262510. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor family C group 6 member A
Gene namesi
Name:Gprc6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:2429498. Gprc6a.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 594574ExtracellularSequence AnalysisAdd
BLAST
Transmembranei595 – 61521HelicalSequence AnalysisAdd
BLAST
Topological domaini616 – 63015CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei631 – 65121HelicalSequence AnalysisAdd
BLAST
Topological domaini652 – 66918ExtracellularSequence AnalysisAdd
BLAST
Transmembranei670 – 69021HelicalSequence AnalysisAdd
BLAST
Topological domaini691 – 70616CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei707 – 72721HelicalSequence AnalysisAdd
BLAST
Topological domaini728 – 75023ExtracellularSequence AnalysisAdd
BLAST
Transmembranei751 – 77121HelicalSequence AnalysisAdd
BLAST
Topological domaini772 – 78413CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei785 – 80521HelicalSequence AnalysisAdd
BLAST
Topological domaini806 – 8127ExtracellularSequence Analysis
Transmembranei813 – 83321HelicalSequence AnalysisAdd
BLAST
Topological domaini834 – 92895CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice shown normal body weight, an increased fat mass, decreased lean body, hyperglycemia and insulin resistance, proteinuria, renal calcium, phosphate wasting, impaired bone mineral density and defective testicular function.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 928908G-protein coupled receptor family C group 6 member APRO_0000043197Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi332 – 3321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi567 – 5671N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8K4Z6.

PTM databases

PhosphoSiteiQ8K4Z6.

Expressioni

Tissue specificityi

Expressed at high level in liver, lung, spleen and heart. Expressed at lower level in kidney, skeletal muscle and brain. Expressed in E7, E11, E15 and E17 embryos.2 Publications

Gene expression databases

BgeeiQ8K4Z6.
GenevestigatoriQ8K4Z6.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

Protein-protein interaction databases

MINTiMINT-4997775.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Z6.
SMRiQ8K4Z6. Positions 34-580, 584-846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295353.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000063697.
HOVERGENiHBG096042.
InParanoidiQ8K4Z6.
KOiK04622.
OMAiETHWAPV.
OrthoDBiEOG7DZ8J8.
PhylomeDBiQ8K4Z6.
TreeFamiTF331269.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8K4Z6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALLITVVTC FMIILDTSQS CHTPDDFVAI TSPGHIMIGG LFAIHEKMLS
60 70 80 90 100
SDDHPRRPQI QKCAGFEISV FLQTLAMIHS IEMINNSTLL SGVKLGYEIY
110 120 130 140 150
DTCTEVTAAM AATLRFLSKF NCSRETVVFQ CDYSSYMPRV KAVIGAGYSE
160 170 180 190 200
TSIAVSRMLN LQLMPQVSYE STAEILSDKI RFPSFLRTVP SDFYQTKAMA
210 220 230 240 250
HLIRQSGWNW IGAITTDDDY GRLALNTFAI QAAENNVCIA FKEVLPAFLS
260 270 280 290 300
DNTIEVRINQ TLEKIIAEAQ VNVIVVFLRK FHVFNLFTKA IERKISKIWI
310 320 330 340 350
ASDNWSTATK IITIPNVKKL GKVVGFAFRR GNTSSFHSFL QTLHMYPNDN
360 370 380 390 400
NKPLHEFAML VSACKYIKDG DLSQCISNYS QATLTYDTTK TIENHLFKRN
410 420 430 440 450
DFLWHYTEPG LIYSIQLAVF ALGHAIRDLC QARDCKKPNA FQPWELLAVL
460 470 480 490 500
KNVTFTDGRN SFHFDAHGDL NTGYDVVLWK ETNGLMTVTK MAEYDLQRDV
510 520 530 540 550
FITTNQETKH EFRKLKQILS KCSKECSPGQ MKKATGSQHS CCYECVSCPE
560 570 580 590 600
NHYSNETDMD HCLLCNNETH WAPVRSTTCF EKEVEYLDWD DSLALLLIAL
610 620 630 640 650
SLLGIAFVLA IGIIFTRNLK TPVVKSSGGL VVCYVMLICH ALNFASTGFF
660 670 680 690 700
IGEPQDFACK TRQTLFGVSF TLCVSCILTK SLKILLAFSF DPKLTMFLKC
710 720 730 740 750
LYRPVPIVLT CTGIQVVICT LWLVLAAPSV EENISLPRVI ILECEEGSAL
760 770 780 790 800
AFGTMLGYIT VLAFICFVFA FKGRKLPENY NEAKFLTFGM LIYFIAWITF
810 820 830 840 850
IPVYTTTFGK YLPAVEIIVI LISNYGILCC IFFPKCYIIL CKQKTNTKSA
860 870 880 890 900
FLQMVYNYSA HSVDSLALSH VSLDSTSYDT ATTNQSPGNK MTACQNDNHL
910 920
PAQVLPHTGT AKTIKASKTL RQKRSSSI
Length:928
Mass (Da):104,213
Last modified:October 1, 2002 - v1
Checksum:iD670E3FEC700BD9B
GO
Isoform 2 (identifier: Q8K4Z6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     271-445: Missing.

Show »
Length:753
Mass (Da):83,999
Checksum:iB9ADC85A644F2CD1
GO
Isoform 3 (identifier: Q8K4Z6-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     446-516: Missing.

Show »
Length:857
Mass (Da):95,968
Checksum:iAF97BC4E8F20E7F7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei271 – 445175Missing in isoform 2. 1 PublicationVSP_016456Add
BLAST
Alternative sequencei446 – 51671Missing in isoform 3. 1 PublicationVSP_016457Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY735399 mRNA. Translation: AAW68012.1.
AY735400 mRNA. Translation: AAW68013.1.
AY735401 mRNA. Translation: AAW68014.1.
AY101365 mRNA. Translation: AAM22280.1.
BC108998 mRNA. Translation: AAI08999.1.
CCDSiCCDS23834.1. [Q8K4Z6-1]
RefSeqiNP_694711.1. NM_153071.1. [Q8K4Z6-1]
UniGeneiMm.221031.

Genome annotation databases

EnsembliENSMUST00000020062; ENSMUSP00000020062; ENSMUSG00000019905. [Q8K4Z6-1]
GeneIDi210198.
KEGGimmu:210198.
UCSCiuc007fau.1. mouse. [Q8K4Z6-1]
uc007fav.1. mouse. [Q8K4Z6-2]
uc007faw.1. mouse. [Q8K4Z6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY735399 mRNA. Translation: AAW68012.1 .
AY735400 mRNA. Translation: AAW68013.1 .
AY735401 mRNA. Translation: AAW68014.1 .
AY101365 mRNA. Translation: AAM22280.1 .
BC108998 mRNA. Translation: AAI08999.1 .
CCDSi CCDS23834.1. [Q8K4Z6-1 ]
RefSeqi NP_694711.1. NM_153071.1. [Q8K4Z6-1 ]
UniGenei Mm.221031.

3D structure databases

ProteinModelPortali Q8K4Z6.
SMRi Q8K4Z6. Positions 34-580, 584-846.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4997775.

Chemistry

GuidetoPHARMACOLOGYi 55.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q8K4Z6.

Proteomic databases

PRIDEi Q8K4Z6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000020062 ; ENSMUSP00000020062 ; ENSMUSG00000019905 . [Q8K4Z6-1 ]
GeneIDi 210198.
KEGGi mmu:210198.
UCSCi uc007fau.1. mouse. [Q8K4Z6-1 ]
uc007fav.1. mouse. [Q8K4Z6-2 ]
uc007faw.1. mouse. [Q8K4Z6-3 ]

Organism-specific databases

CTDi 222545.
MGIi MGI:2429498. Gprc6a.

Phylogenomic databases

eggNOGi NOG295353.
GeneTreei ENSGT00760000118884.
HOGENOMi HOG000063697.
HOVERGENi HBG096042.
InParanoidi Q8K4Z6.
KOi K04622.
OMAi ETHWAPV.
OrthoDBi EOG7DZ8J8.
PhylomeDBi Q8K4Z6.
TreeFami TF331269.

Enzyme and pathway databases

Reactomei REACT_207651. G alpha (q) signalling events.
REACT_262510. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

NextBioi 372908.
PROi Q8K4Z6.
SOURCEi Search...

Gene expression databases

Bgeei Q8K4Z6.
Genevestigatori Q8K4Z6.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view ]
PRINTSi PR00248. GPCRMGR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a family C orphan G-protein coupled receptor."
    Kuang D., Yao Y., Lam J., Tsushima R.G., Hampson D.R.
    J. Neurochem. 93:383-391(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION, SUBUNIT, GLYCOSYLATION, TISSUE SPECIFICITY.
    Strain: CD-1.
  2. "Cloning of a metabotropic glutamate/pheromone receptor (Gprc6a)."
    Lorenz-Depiereux B., Dorner M., Strom T.M.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster / NIH.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Deorphanization of GPRC6A: a promiscuous L-alpha-amino acid receptor with preference for basic amino acids."
    Wellendorph P., Hansen K.B., Balsgaard A., Greenwood J.R., Egebjerg J., Brauener-Osborne H.
    Mol. Pharmacol. 67:589-597(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "GPRC6A mediates the non-genomic effects of steroids."
    Pi M., Parrill A.L., Quarles L.D.
    J. Biol. Chem. 285:39953-39964(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGPC6A_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Z6
Secondary accession number(s): Q5DK51, Q5DK52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3