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Protein

G-protein coupled receptor family C group 6 member A

Gene

Gprc6a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor activated by amino acids with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine but also by small and polar amino acids. The L-alpha amino acids respond is augmented by divalent cations Ca2+ and Mg2+. Activated by extracellular calcium and osteocalin. Seems to act through a G(q)/G(11) and G(i)-coupled pathway. Mediates the non-genomic effects of androgens in multiple tissue. May coordinates nutritional and hormonal anabolic signals through the sensing of extracellular amino acids, osteocalcin, divalents ions and its responsiveness to anabolic steroids.4 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: MGI

GO - Biological processi

  • calcium-mediated signaling Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • response to amino acid Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-416476. G alpha (q) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor family C group 6 member A
Gene namesi
Name:Gprc6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2429498. Gprc6a.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 594ExtracellularSequence analysisAdd BLAST574
Transmembranei595 – 615HelicalSequence analysisAdd BLAST21
Topological domaini616 – 630CytoplasmicSequence analysisAdd BLAST15
Transmembranei631 – 651HelicalSequence analysisAdd BLAST21
Topological domaini652 – 669ExtracellularSequence analysisAdd BLAST18
Transmembranei670 – 690HelicalSequence analysisAdd BLAST21
Topological domaini691 – 706CytoplasmicSequence analysisAdd BLAST16
Transmembranei707 – 727HelicalSequence analysisAdd BLAST21
Topological domaini728 – 750ExtracellularSequence analysisAdd BLAST23
Transmembranei751 – 771HelicalSequence analysisAdd BLAST21
Topological domaini772 – 784CytoplasmicSequence analysisAdd BLAST13
Transmembranei785 – 805HelicalSequence analysisAdd BLAST21
Topological domaini806 – 812ExtracellularSequence analysis7
Transmembranei813 – 833HelicalSequence analysisAdd BLAST21
Topological domaini834 – 928CytoplasmicSequence analysisAdd BLAST95

GO - Cellular componenti

  • cell surface Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice shown normal body weight, an increased fat mass, decreased lean body, hyperglycemia and insulin resistance, proteinuria, renal calcium, phosphate wasting, impaired bone mineral density and defective testicular function.2 Publications

Chemistry databases

ChEMBLiCHEMBL3347255.
GuidetoPHARMACOLOGYi55.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000004319721 – 928G-protein coupled receptor family C group 6 member AAdd BLAST908

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi332N-linked (GlcNAc...)Sequence analysis1
Glycosylationi555N-linked (GlcNAc...)Sequence analysis1
Glycosylationi567N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8K4Z6.
PRIDEiQ8K4Z6.

PTM databases

PhosphoSitePlusiQ8K4Z6.

Expressioni

Tissue specificityi

Expressed at high level in liver, lung, spleen and heart. Expressed at lower level in kidney, skeletal muscle and brain. Expressed in E7, E11, E15 and E17 embryos.2 Publications

Gene expression databases

BgeeiENSMUSG00000019905.
GenevisibleiQ8K4Z6. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

Protein-protein interaction databases

MINTiMINT-4997775.
STRINGi10090.ENSMUSP00000020062.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Z6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118974.
HOGENOMiHOG000063697.
HOVERGENiHBG096042.
InParanoidiQ8K4Z6.
KOiK04622.
OMAiETHWAPV.
OrthoDBiEOG091G01NA.
PhylomeDBiQ8K4Z6.
TreeFamiTF331269.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8K4Z6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALLITVVTC FMIILDTSQS CHTPDDFVAI TSPGHIMIGG LFAIHEKMLS
60 70 80 90 100
SDDHPRRPQI QKCAGFEISV FLQTLAMIHS IEMINNSTLL SGVKLGYEIY
110 120 130 140 150
DTCTEVTAAM AATLRFLSKF NCSRETVVFQ CDYSSYMPRV KAVIGAGYSE
160 170 180 190 200
TSIAVSRMLN LQLMPQVSYE STAEILSDKI RFPSFLRTVP SDFYQTKAMA
210 220 230 240 250
HLIRQSGWNW IGAITTDDDY GRLALNTFAI QAAENNVCIA FKEVLPAFLS
260 270 280 290 300
DNTIEVRINQ TLEKIIAEAQ VNVIVVFLRK FHVFNLFTKA IERKISKIWI
310 320 330 340 350
ASDNWSTATK IITIPNVKKL GKVVGFAFRR GNTSSFHSFL QTLHMYPNDN
360 370 380 390 400
NKPLHEFAML VSACKYIKDG DLSQCISNYS QATLTYDTTK TIENHLFKRN
410 420 430 440 450
DFLWHYTEPG LIYSIQLAVF ALGHAIRDLC QARDCKKPNA FQPWELLAVL
460 470 480 490 500
KNVTFTDGRN SFHFDAHGDL NTGYDVVLWK ETNGLMTVTK MAEYDLQRDV
510 520 530 540 550
FITTNQETKH EFRKLKQILS KCSKECSPGQ MKKATGSQHS CCYECVSCPE
560 570 580 590 600
NHYSNETDMD HCLLCNNETH WAPVRSTTCF EKEVEYLDWD DSLALLLIAL
610 620 630 640 650
SLLGIAFVLA IGIIFTRNLK TPVVKSSGGL VVCYVMLICH ALNFASTGFF
660 670 680 690 700
IGEPQDFACK TRQTLFGVSF TLCVSCILTK SLKILLAFSF DPKLTMFLKC
710 720 730 740 750
LYRPVPIVLT CTGIQVVICT LWLVLAAPSV EENISLPRVI ILECEEGSAL
760 770 780 790 800
AFGTMLGYIT VLAFICFVFA FKGRKLPENY NEAKFLTFGM LIYFIAWITF
810 820 830 840 850
IPVYTTTFGK YLPAVEIIVI LISNYGILCC IFFPKCYIIL CKQKTNTKSA
860 870 880 890 900
FLQMVYNYSA HSVDSLALSH VSLDSTSYDT ATTNQSPGNK MTACQNDNHL
910 920
PAQVLPHTGT AKTIKASKTL RQKRSSSI
Length:928
Mass (Da):104,213
Last modified:October 1, 2002 - v1
Checksum:iD670E3FEC700BD9B
GO
Isoform 2 (identifier: Q8K4Z6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     271-445: Missing.

Show »
Length:753
Mass (Da):83,999
Checksum:iB9ADC85A644F2CD1
GO
Isoform 3 (identifier: Q8K4Z6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     446-516: Missing.

Show »
Length:857
Mass (Da):95,968
Checksum:iAF97BC4E8F20E7F7
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016456271 – 445Missing in isoform 2. 1 PublicationAdd BLAST175
Alternative sequenceiVSP_016457446 – 516Missing in isoform 3. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY735399 mRNA. Translation: AAW68012.1.
AY735400 mRNA. Translation: AAW68013.1.
AY735401 mRNA. Translation: AAW68014.1.
AY101365 mRNA. Translation: AAM22280.1.
BC108998 mRNA. Translation: AAI08999.1.
CCDSiCCDS23834.1. [Q8K4Z6-1]
RefSeqiNP_694711.1. NM_153071.1. [Q8K4Z6-1]
UniGeneiMm.221031.

Genome annotation databases

EnsembliENSMUST00000020062; ENSMUSP00000020062; ENSMUSG00000019905. [Q8K4Z6-1]
GeneIDi210198.
KEGGimmu:210198.
UCSCiuc007fau.1. mouse. [Q8K4Z6-1]
uc007fav.1. mouse. [Q8K4Z6-2]
uc007faw.1. mouse. [Q8K4Z6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY735399 mRNA. Translation: AAW68012.1.
AY735400 mRNA. Translation: AAW68013.1.
AY735401 mRNA. Translation: AAW68014.1.
AY101365 mRNA. Translation: AAM22280.1.
BC108998 mRNA. Translation: AAI08999.1.
CCDSiCCDS23834.1. [Q8K4Z6-1]
RefSeqiNP_694711.1. NM_153071.1. [Q8K4Z6-1]
UniGeneiMm.221031.

3D structure databases

ProteinModelPortaliQ8K4Z6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997775.
STRINGi10090.ENSMUSP00000020062.

Chemistry databases

ChEMBLiCHEMBL3347255.
GuidetoPHARMACOLOGYi55.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiQ8K4Z6.

Proteomic databases

PaxDbiQ8K4Z6.
PRIDEiQ8K4Z6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020062; ENSMUSP00000020062; ENSMUSG00000019905. [Q8K4Z6-1]
GeneIDi210198.
KEGGimmu:210198.
UCSCiuc007fau.1. mouse. [Q8K4Z6-1]
uc007fav.1. mouse. [Q8K4Z6-2]
uc007faw.1. mouse. [Q8K4Z6-3]

Organism-specific databases

CTDi222545.
MGIiMGI:2429498. Gprc6a.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118974.
HOGENOMiHOG000063697.
HOVERGENiHBG096042.
InParanoidiQ8K4Z6.
KOiK04622.
OMAiETHWAPV.
OrthoDBiEOG091G01NA.
PhylomeDBiQ8K4Z6.
TreeFamiTF331269.

Enzyme and pathway databases

ReactomeiR-MMU-416476. G alpha (q) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

PROiQ8K4Z6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019905.
GenevisibleiQ8K4Z6. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPC6A_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Z6
Secondary accession number(s): Q5DK51, Q5DK52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2002
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.