Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Splicing factor 3A subunit 1

Gene

Sf3a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei169Critical for binding to SF3A3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 1
Alternative name(s):
SF3a120
Gene namesi
Name:Sf3a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914715. Sf3a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001149182 – 791Splicing factor 3A subunit 1Add BLAST790

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei55N6-acetyllysineCombined sources1
Modified residuei320PhosphoserineBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei357PhosphoserineBy similarity1
Modified residuei411PhosphoserineBy similarity1
Modified residuei449PhosphoserineBy similarity1
Modified residuei454PhosphotyrosineBy similarity1
Modified residuei506PhosphoserineBy similarity1
Cross-linki540Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki684Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei757PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8K4Z5.
MaxQBiQ8K4Z5.
PaxDbiQ8K4Z5.
PeptideAtlasiQ8K4Z5.
PRIDEiQ8K4Z5.

PTM databases

iPTMnetiQ8K4Z5.
PhosphoSitePlusiQ8K4Z5.
SwissPalmiQ8K4Z5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002129.
CleanExiMM_SF3A1.
GenevisibleiQ8K4Z5. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex (By similarity). Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi212207. 37 interactors.
IntActiQ8K4Z5. 38 interactors.
MINTiMINT-1868050.
STRINGi10090.ENSMUSP00000002198.

Structurei

Secondary structure

1791
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi685 – 687Combined sources3
Helixi692 – 698Combined sources7
Beta strandi703 – 709Combined sources7
Beta strandi713 – 715Combined sources3
Beta strandi721 – 729Combined sources9
Helixi736 – 745Combined sources10
Turni750 – 752Combined sources3
Beta strandi753 – 757Combined sources5
Beta strandi760 – 762Combined sources3
Helixi768 – 771Combined sources4
Beta strandi778 – 783Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WE7NMR-A685-786[»]
ProteinModelPortaliQ8K4Z5.
SMRiQ8K4Z5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8K4Z5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati52 – 94SURP motif 1Add BLAST43
Repeati166 – 208SURP motif 2Add BLAST43
Domaini705 – 788Ubiquitin-likePROSITE-ProRule annotationAdd BLAST84

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 14Poly-Pro5
Compositional biasi118 – 122Poly-Gln5
Compositional biasi260 – 267Poly-Glu8
Compositional biasi367 – 370Poly-Pro4
Compositional biasi555 – 558Poly-Pro4
Compositional biasi670 – 673Poly-Pro4

Domaini

SURP motif 2 mediates direct binding to SF3A3.By similarity

Sequence similaritiesi

Contains 2 SURP motif repeats.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0007. Eukaryota.
ENOG410XPNW. LUCA.
GeneTreeiENSGT00730000111077.
HOGENOMiHOG000238941.
HOVERGENiHBG059993.
InParanoidiQ8K4Z5.
KOiK12825.
OMAiNEMPQPP.
OrthoDBiEOG091G0539.
PhylomeDBiQ8K4Z5.
TreeFamiTF105705.

Family and domain databases

InterProiIPR022030. SF3A1.
IPR000061. Surp.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF109905. SSF109905. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8K4Z5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAGPVQAVP PPPPVATESK QPIEEEASSK EDPTPSKPVV GIIYPPPEVR
60 70 80 90 100
NIVDKTASFV ARNGPEFEAR IRQNEINNPK FNFLNPNDPY HAYYRHKVSE
110 120 130 140 150
FKEGKAQEPS AAIPKVMQQQ QQATQQQLPQ KVQAQVIQET IVPKEPPPEF
160 170 180 190 200
EFIADPPSIS AFDLDVVKLT AQFVARNGRQ FLTQLMQKEQ RNYQFDFLRP
210 220 230 240 250
QHSLFNYFTK LVEQYTKILI PPKGLFSKLK KEAENPREVL DQVCYRVEWA
260 270 280 290 300
KFQERERKKE EEEKEKERVA YAQIDWHDFV VVETVDFQPN EQGNFPPPTT
310 320 330 340 350
PEELGARILI QERYEKFGES EEVEMEVESD EEDQEKAEET PSQLDQDTQV
360 370 380 390 400
QDMDEGSDDE EEGQKVPPPP ETPMPPPLPP TPDQVIVRKD YDPKASKPLP
410 420 430 440 450
PAPAPDEYLV SPITGEKIPA SKMQEHMRIG LLDPRWLEQR DRSIREKQSD
460 470 480 490 500
DEVYAPGLDI ESSLKQLAER RTDIFGVEET AIGKKIGEEE IQKPEEKVTW
510 520 530 540 550
DGHSGSMART QQAAQANITL QEQIEAIHKA KGLVPEDDTK EKIGPSKPNE
560 570 580 590 600
IPQQPPPPSS ATNIPSSAPP ITSVPRPPAM PPPVRTTVVS AVPVMPRPPM
610 620 630 640 650
ASVVRLPPGS VIAPMPPIIH APRINVVPMP PAAPPIMAPR PPPMIVPTAF
660 670 680 690 700
VPAPPVAPVP APAPMPPVHP PPPMEDEPPS KKLKTEDSLM PEEEFLRRNK
710 720 730 740 750
GPVSIKVQVP NMQDKTEWKL NGQGLVFTLP LTDQVSVIKV KIHEATGMPA
760 770 780 790
GKQKLQYEGI FIKDSNSLAY YNMASGAVIH LALKERGGRK K
Length:791
Mass (Da):88,545
Last modified:October 1, 2002 - v1
Checksum:iD83D0432469C3708
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti257R → G in BAC26142 (PubMed:16141072).Curated1
Sequence conflicti368P → L in BAC26294 (PubMed:16141072).Curated1
Sequence conflicti708Q → L in BAC26853 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028829 mRNA. Translation: BAC26142.1.
AK029095 mRNA. Translation: BAC26294.1.
AK030223 mRNA. Translation: BAC26853.1.
AL807825 Genomic DNA. Translation: CAI25745.1.
BC010727 mRNA. Translation: AAH10727.1.
BC029753 mRNA. Translation: AAH29753.1.
CCDSiCCDS24380.1.
RefSeqiNP_080451.4. NM_026175.5.
UniGeneiMm.156914.

Genome annotation databases

EnsembliENSMUST00000002198; ENSMUSP00000002198; ENSMUSG00000002129.
GeneIDi67465.
KEGGimmu:67465.
UCSCiuc007hup.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028829 mRNA. Translation: BAC26142.1.
AK029095 mRNA. Translation: BAC26294.1.
AK030223 mRNA. Translation: BAC26853.1.
AL807825 Genomic DNA. Translation: CAI25745.1.
BC010727 mRNA. Translation: AAH10727.1.
BC029753 mRNA. Translation: AAH29753.1.
CCDSiCCDS24380.1.
RefSeqiNP_080451.4. NM_026175.5.
UniGeneiMm.156914.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WE7NMR-A685-786[»]
ProteinModelPortaliQ8K4Z5.
SMRiQ8K4Z5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212207. 37 interactors.
IntActiQ8K4Z5. 38 interactors.
MINTiMINT-1868050.
STRINGi10090.ENSMUSP00000002198.

PTM databases

iPTMnetiQ8K4Z5.
PhosphoSitePlusiQ8K4Z5.
SwissPalmiQ8K4Z5.

Proteomic databases

EPDiQ8K4Z5.
MaxQBiQ8K4Z5.
PaxDbiQ8K4Z5.
PeptideAtlasiQ8K4Z5.
PRIDEiQ8K4Z5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002198; ENSMUSP00000002198; ENSMUSG00000002129.
GeneIDi67465.
KEGGimmu:67465.
UCSCiuc007hup.2. mouse.

Organism-specific databases

CTDi10291.
MGIiMGI:1914715. Sf3a1.

Phylogenomic databases

eggNOGiKOG0007. Eukaryota.
ENOG410XPNW. LUCA.
GeneTreeiENSGT00730000111077.
HOGENOMiHOG000238941.
HOVERGENiHBG059993.
InParanoidiQ8K4Z5.
KOiK12825.
OMAiNEMPQPP.
OrthoDBiEOG091G0539.
PhylomeDBiQ8K4Z5.
TreeFamiTF105705.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSf3a1. mouse.
EvolutionaryTraceiQ8K4Z5.
PROiQ8K4Z5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002129.
CleanExiMM_SF3A1.
GenevisibleiQ8K4Z5. MM.

Family and domain databases

InterProiIPR022030. SF3A1.
IPR000061. Surp.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF109905. SSF109905. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSF3A1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Z5
Secondary accession number(s): Q8C0M7
, Q8C128, Q8C175, Q921T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.