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Q8K4Z5

- SF3A1_MOUSE

UniProt

Q8K4Z5 - SF3A1_MOUSE

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Protein

Splicing factor 3A subunit 1

Gene
Sf3a1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei169 – 1691Critical for binding to SF3A3 By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 1
Alternative name(s):
SF3a120
Gene namesi
Name:Sf3a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1914715. Sf3a1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. catalytic step 2 spliceosome Source: Ensembl
  2. U2-type spliceosomal complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 791791Splicing factor 3A subunit 1PRO_0000114918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551N6-acetyllysine1 Publication
Modified residuei320 – 3201Phosphoserine By similarity
Modified residuei329 – 3291Phosphoserine By similarity
Modified residuei357 – 3571Phosphoserine By similarity
Modified residuei411 – 4111Phosphoserine By similarity
Modified residuei449 – 4491Phosphoserine By similarity
Modified residuei454 – 4541Phosphotyrosine By similarity
Modified residuei757 – 7571Phosphotyrosine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8K4Z5.
PaxDbiQ8K4Z5.
PRIDEiQ8K4Z5.

PTM databases

PhosphoSiteiQ8K4Z5.

Expressioni

Gene expression databases

BgeeiQ8K4Z5.
CleanExiMM_SF3A1.
GenevestigatoriQ8K4Z5.

Interactioni

Subunit structurei

Identified in the spliceosome C complex By similarity. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A3 By similarity.

Protein-protein interaction databases

BioGridi212207. 36 interactions.
IntActiQ8K4Z5. 37 interactions.
MINTiMINT-1868050.

Structurei

Secondary structure

1
791
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi685 – 6873
Helixi692 – 6987
Beta strandi703 – 7097
Beta strandi713 – 7153
Beta strandi721 – 7299
Helixi736 – 74510
Turni750 – 7523
Beta strandi753 – 7575
Beta strandi760 – 7623
Helixi768 – 7714
Beta strandi778 – 7836

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WE7NMR-A685-786[»]
ProteinModelPortaliQ8K4Z5.
SMRiQ8K4Z5. Positions 48-110, 134-288, 685-788.

Miscellaneous databases

EvolutionaryTraceiQ8K4Z5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati52 – 9443SURP motif 1Add
BLAST
Repeati166 – 20843SURP motif 2Add
BLAST
Domaini705 – 78884Ubiquitin-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 145Poly-Pro
Compositional biasi118 – 1225Poly-Gln
Compositional biasi260 – 2678Poly-Glu
Compositional biasi367 – 3704Poly-Pro
Compositional biasi555 – 5584Poly-Pro
Compositional biasi670 – 6734Poly-Pro

Domaini

SURP motif 2 mediates direct binding to SF3A3 By similarity.

Sequence similaritiesi

Contains 2 SURP motif repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG300902.
GeneTreeiENSGT00730000111077.
HOGENOMiHOG000238941.
HOVERGENiHBG059993.
InParanoidiQ8K4Z5.
KOiK12825.
OMAiVMQQQQT.
OrthoDBiEOG7JDQX9.
PhylomeDBiQ8K4Z5.
TreeFamiTF105705.

Family and domain databases

InterProiIPR022030. PRP21-like.
IPR000061. Surp.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF109905. SSF109905. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8K4Z5-1 [UniParc]FASTAAdd to Basket

« Hide

MQAGPVQAVP PPPPVATESK QPIEEEASSK EDPTPSKPVV GIIYPPPEVR    50
NIVDKTASFV ARNGPEFEAR IRQNEINNPK FNFLNPNDPY HAYYRHKVSE 100
FKEGKAQEPS AAIPKVMQQQ QQATQQQLPQ KVQAQVIQET IVPKEPPPEF 150
EFIADPPSIS AFDLDVVKLT AQFVARNGRQ FLTQLMQKEQ RNYQFDFLRP 200
QHSLFNYFTK LVEQYTKILI PPKGLFSKLK KEAENPREVL DQVCYRVEWA 250
KFQERERKKE EEEKEKERVA YAQIDWHDFV VVETVDFQPN EQGNFPPPTT 300
PEELGARILI QERYEKFGES EEVEMEVESD EEDQEKAEET PSQLDQDTQV 350
QDMDEGSDDE EEGQKVPPPP ETPMPPPLPP TPDQVIVRKD YDPKASKPLP 400
PAPAPDEYLV SPITGEKIPA SKMQEHMRIG LLDPRWLEQR DRSIREKQSD 450
DEVYAPGLDI ESSLKQLAER RTDIFGVEET AIGKKIGEEE IQKPEEKVTW 500
DGHSGSMART QQAAQANITL QEQIEAIHKA KGLVPEDDTK EKIGPSKPNE 550
IPQQPPPPSS ATNIPSSAPP ITSVPRPPAM PPPVRTTVVS AVPVMPRPPM 600
ASVVRLPPGS VIAPMPPIIH APRINVVPMP PAAPPIMAPR PPPMIVPTAF 650
VPAPPVAPVP APAPMPPVHP PPPMEDEPPS KKLKTEDSLM PEEEFLRRNK 700
GPVSIKVQVP NMQDKTEWKL NGQGLVFTLP LTDQVSVIKV KIHEATGMPA 750
GKQKLQYEGI FIKDSNSLAY YNMASGAVIH LALKERGGRK K 791
Length:791
Mass (Da):88,545
Last modified:October 1, 2002 - v1
Checksum:iD83D0432469C3708
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti257 – 2571R → G in BAC26142. 1 Publication
Sequence conflicti368 – 3681P → L in BAC26294. 1 Publication
Sequence conflicti708 – 7081Q → L in BAC26853. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK028829 mRNA. Translation: BAC26142.1.
AK029095 mRNA. Translation: BAC26294.1.
AK030223 mRNA. Translation: BAC26853.1.
AL807825 Genomic DNA. Translation: CAI25745.1.
BC010727 mRNA. Translation: AAH10727.1.
BC029753 mRNA. Translation: AAH29753.1.
CCDSiCCDS24380.1.
RefSeqiNP_080451.4. NM_026175.5.
UniGeneiMm.156914.

Genome annotation databases

EnsembliENSMUST00000002198; ENSMUSP00000002198; ENSMUSG00000002129.
GeneIDi67465.
KEGGimmu:67465.
UCSCiuc007hup.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK028829 mRNA. Translation: BAC26142.1 .
AK029095 mRNA. Translation: BAC26294.1 .
AK030223 mRNA. Translation: BAC26853.1 .
AL807825 Genomic DNA. Translation: CAI25745.1 .
BC010727 mRNA. Translation: AAH10727.1 .
BC029753 mRNA. Translation: AAH29753.1 .
CCDSi CCDS24380.1.
RefSeqi NP_080451.4. NM_026175.5.
UniGenei Mm.156914.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WE7 NMR - A 685-786 [» ]
ProteinModelPortali Q8K4Z5.
SMRi Q8K4Z5. Positions 48-110, 134-288, 685-788.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 212207. 36 interactions.
IntActi Q8K4Z5. 37 interactions.
MINTi MINT-1868050.

PTM databases

PhosphoSitei Q8K4Z5.

Proteomic databases

MaxQBi Q8K4Z5.
PaxDbi Q8K4Z5.
PRIDEi Q8K4Z5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002198 ; ENSMUSP00000002198 ; ENSMUSG00000002129 .
GeneIDi 67465.
KEGGi mmu:67465.
UCSCi uc007hup.2. mouse.

Organism-specific databases

CTDi 10291.
MGIi MGI:1914715. Sf3a1.

Phylogenomic databases

eggNOGi NOG300902.
GeneTreei ENSGT00730000111077.
HOGENOMi HOG000238941.
HOVERGENi HBG059993.
InParanoidi Q8K4Z5.
KOi K12825.
OMAi VMQQQQT.
OrthoDBi EOG7JDQX9.
PhylomeDBi Q8K4Z5.
TreeFami TF105705.

Miscellaneous databases

ChiTaRSi SF3A1. mouse.
EvolutionaryTracei Q8K4Z5.
NextBioi 324654.
PROi Q8K4Z5.
SOURCEi Search...

Gene expression databases

Bgeei Q8K4Z5.
CleanExi MM_SF3A1.
Genevestigatori Q8K4Z5.

Family and domain databases

InterProi IPR022030. PRP21-like.
IPR000061. Surp.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view ]
SMARTi SM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF109905. SSF109905. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEi PS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland and Mammary tumor.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 238-246; 471-484 AND 755-763, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. "Solution structure of ubiquitin-like domain in Sf3a120."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 685-786.

Entry informationi

Entry nameiSF3A1_MOUSE
AccessioniPrimary (citable) accession number: Q8K4Z5
Secondary accession number(s): Q8C0M7
, Q8C128, Q8C175, Q921T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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