Q8K4Z5 (SF3A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Splicing factor 3A subunit 1

Alternative name(s):
SF3a120

Gene names

Name:

Sf3a1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	791 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex By similarity.
Subunit structure	Identified in the spliceosome C complex By similarity. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A3 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Contains 2 SURP motif repeats. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mRNA splicing, via spliceosome Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	U2-type spliceosomal complex Inferred from sequence or structural similarity. Source: UniProtKB catalytic step 2 spliceosome Inferred from electronic annotation. Source: Compara
Molecular_function	RNA binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 791

791

Splicing factor 3A subunit 1

PRO_0000114918

Regions

Repeat

52 – 94

43

SURP motif 1

Repeat

166 – 208

43

SURP motif 2

Domain

705 – 788

84

Ubiquitin-like

Compositional bias

10 – 14

5

Poly-Pro

Compositional bias

118 – 122

5

Poly-Gln

Compositional bias

260 – 267

8

Poly-Glu

Compositional bias

367 – 370

4

Poly-Pro

Compositional bias

555 – 558

4

Poly-Pro

Compositional bias

670 – 673

4

Poly-Pro

Amino acid modifications

Modified residue

55

1

N6-acetyllysine By similarity

Modified residue

320

1

Phosphoserine By similarity

Modified residue

329

1

Phosphoserine Ref.5

Modified residue

357

1

Phosphoserine By similarity

Modified residue

411

1

Phosphoserine By similarity

Modified residue

449

1

Phosphoserine By similarity

Modified residue

454

1

Phosphotyrosine By similarity

Modified residue

757

1

Phosphotyrosine By similarity

Experimental info

Sequence conflict

257

1

R → G in BAC26142. Ref.1

Sequence conflict

368

1

P → L in BAC26294. Ref.1

Sequence conflict

708

1

Q → L in BAC26853. Ref.1

Secondary structure

1

.

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791

Helix Strand Turn

Details...

Helix

685 – 687

3

Helix

692 – 698

7

Beta strand

703 – 709

7

Beta strand

713 – 715

3

Beta strand

721 – 729

9

Helix

736 – 745

10

Turn

750 – 752

3

Beta strand

753 – 757

5

Beta strand

760 – 762

3

Helix

768 – 771

4

Beta strand

778 – 783

6

Sequences

	Sequence		Length	Mass (Da)
	Q8K4Z5 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: D83D0432469C3708	FASTA	791	88,545
	10 20 30 40 50 60 MQAGPVQAVP PPPPVATESK QPIEEEASSK EDPTPSKPVV GIIYPPPEVR NIVDKTASFV 70 80 90 100 110 120 ARNGPEFEAR IRQNEINNPK FNFLNPNDPY HAYYRHKVSE FKEGKAQEPS AAIPKVMQQQ 130 140 150 160 170 180 QQATQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT AQFVARNGRQ 190 200 210 220 230 240 FLTQLMQKEQ RNYQFDFLRP QHSLFNYFTK LVEQYTKILI PPKGLFSKLK KEAENPREVL 250 260 270 280 290 300 DQVCYRVEWA KFQERERKKE EEEKEKERVA YAQIDWHDFV VVETVDFQPN EQGNFPPPTT 310 320 330 340 350 360 PEELGARILI QERYEKFGES EEVEMEVESD EEDQEKAEET PSQLDQDTQV QDMDEGSDDE 370 380 390 400 410 420 EEGQKVPPPP ETPMPPPLPP TPDQVIVRKD YDPKASKPLP PAPAPDEYLV SPITGEKIPA 430 440 450 460 470 480 SKMQEHMRIG LLDPRWLEQR DRSIREKQSD DEVYAPGLDI ESSLKQLAER RTDIFGVEET 490 500 510 520 530 540 AIGKKIGEEE IQKPEEKVTW DGHSGSMART QQAAQANITL QEQIEAIHKA KGLVPEDDTK 550 560 570 580 590 600 EKIGPSKPNE IPQQPPPPSS ATNIPSSAPP ITSVPRPPAM PPPVRTTVVS AVPVMPRPPM 610 620 630 640 650 660 ASVVRLPPGS VIAPMPPIIH APRINVVPMP PAAPPIMAPR PPPMIVPTAF VPAPPVAPVP 670 680 690 700 710 720 APAPMPPVHP PPPMEDEPPS KKLKTEDSLM PEEEFLRRNK GPVSIKVQVP NMQDKTEWKL 730 740 750 760 770 780 NGQGLVFTLP LTDQVSVIKV KIHEATGMPA GKQKLQYEGI FIKDSNSLAY YNMASGAVIH 790 LALKERGGRK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Skin and Testis.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland and Mammary tumor.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 238-246; 471-484 AND 755-763, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Solution structure of ubiquitin-like domain in Sf3a120." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 685-786.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK028829 mRNA. Translation: BAC26142.1.
AK029095 mRNA. Translation: BAC26294.1.
AK030223 mRNA. Translation: BAC26853.1.
AL807825 Genomic DNA. Translation: CAI25745.1.
BC010727 mRNA. Translation: AAH10727.1.
BC029753 mRNA. Translation: AAH29753.1.

IPI

IPI00408796.

RefSeq

NP_080451.4. NM_026175.5.

UniGene

Mm.156914.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WE7	NMR	-	A	685-786	[»]

ProteinModelPortal

Q8K4Z5.

SMR

Q8K4Z5. Positions 48-110, 134-288, 685-788.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8K4Z5. 35 interactions.

PTM databases

PhosphoSite

Q8K4Z5.

Proteomic databases

PaxDb

Q8K4Z5.

PRIDE

Q8K4Z5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000002198; ENSMUSP00000002198; ENSMUSG00000002129.

GeneID

67465.

KEGG

mmu:67465.

UCSC

uc007hup.2. mouse.

Organism-specific databases

CTD

10291.

MGI

MGI:1914715. Sf3a1.

Phylogenomic databases

eggNOG

NOG300902.

GeneTree

ENSGT00570000079144.

HOGENOM

HOG000238941.

HOVERGEN

HBG059993.

InParanoid

Q8K4Z5.

KO

K12825.

OMA

GMPPAKQ.

OrthoDB

EOG4N5VWN.

Gene expression databases

Bgee

Q8K4Z5.

CleanEx

MM_SF3A1.

Genevestigator

Q8K4Z5.

GermOnline

ENSMUSG00000002129. Mus musculus.

Family and domain databases

InterPro

IPR022030. PRP21-like.
IPR000061. Surp.
IPR000626. Ubiquitin.
IPR019955. Ubiquitin_supergroup.
[Graphical view]

Pfam

PF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view]

SMART

SM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]

SUPFAM

SSF109905. SSF109905. 2 hits.

PROSITE

PS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

SF3A1. mouse.

EvolutionaryTrace

Q8K4Z5.

NextBio

324654.

SOURCE

Search...

Entry information

Entry name

SF3A1_MOUSE

Accession

Primary (citable) accession number: Q8K4Z5
Secondary accession number(s): Q8C0M7 , Q8C128, Q8C175, Q921T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program