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Q8K4Z5 (SF3A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Splicing factor 3A subunit 1
Alternative name(s):
SF3a120
Gene names
Name:Sf3a1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length791 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex By similarity.

Subunit structure

Identified in the spliceosome C complex By similarity. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A3 By similarity.

Subcellular location

Nucleus By similarity.

Domain

SURP motif 2 mediates direct binding to SF3A3 By similarity.

Sequence similarities

Contains 2 SURP motif repeats.

Contains 1 ubiquitin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 791791Splicing factor 3A subunit 1
PRO_0000114918

Regions

Repeat52 – 9443SURP motif 1
Repeat166 – 20843SURP motif 2
Domain705 – 78884Ubiquitin-like
Compositional bias10 – 145Poly-Pro
Compositional bias118 – 1225Poly-Gln
Compositional bias260 – 2678Poly-Glu
Compositional bias367 – 3704Poly-Pro
Compositional bias555 – 5584Poly-Pro
Compositional bias670 – 6734Poly-Pro

Sites

Site1691Critical for binding to SF3A3 By similarity

Amino acid modifications

Modified residue551N6-acetyllysine Ref.5
Modified residue3201Phosphoserine By similarity
Modified residue3291Phosphoserine By similarity
Modified residue3571Phosphoserine By similarity
Modified residue4111Phosphoserine By similarity
Modified residue4491Phosphoserine By similarity
Modified residue4541Phosphotyrosine By similarity
Modified residue7571Phosphotyrosine By similarity

Experimental info

Sequence conflict2571R → G in BAC26142. Ref.1
Sequence conflict3681P → L in BAC26294. Ref.1
Sequence conflict7081Q → L in BAC26853. Ref.1

Secondary structure

...................... 791
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8K4Z5 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: D83D0432469C3708

FASTA79188,545
        10         20         30         40         50         60 
MQAGPVQAVP PPPPVATESK QPIEEEASSK EDPTPSKPVV GIIYPPPEVR NIVDKTASFV 

        70         80         90        100        110        120 
ARNGPEFEAR IRQNEINNPK FNFLNPNDPY HAYYRHKVSE FKEGKAQEPS AAIPKVMQQQ 

       130        140        150        160        170        180 
QQATQQQLPQ KVQAQVIQET IVPKEPPPEF EFIADPPSIS AFDLDVVKLT AQFVARNGRQ 

       190        200        210        220        230        240 
FLTQLMQKEQ RNYQFDFLRP QHSLFNYFTK LVEQYTKILI PPKGLFSKLK KEAENPREVL 

       250        260        270        280        290        300 
DQVCYRVEWA KFQERERKKE EEEKEKERVA YAQIDWHDFV VVETVDFQPN EQGNFPPPTT 

       310        320        330        340        350        360 
PEELGARILI QERYEKFGES EEVEMEVESD EEDQEKAEET PSQLDQDTQV QDMDEGSDDE 

       370        380        390        400        410        420 
EEGQKVPPPP ETPMPPPLPP TPDQVIVRKD YDPKASKPLP PAPAPDEYLV SPITGEKIPA 

       430        440        450        460        470        480 
SKMQEHMRIG LLDPRWLEQR DRSIREKQSD DEVYAPGLDI ESSLKQLAER RTDIFGVEET 

       490        500        510        520        530        540 
AIGKKIGEEE IQKPEEKVTW DGHSGSMART QQAAQANITL QEQIEAIHKA KGLVPEDDTK 

       550        560        570        580        590        600 
EKIGPSKPNE IPQQPPPPSS ATNIPSSAPP ITSVPRPPAM PPPVRTTVVS AVPVMPRPPM 

       610        620        630        640        650        660 
ASVVRLPPGS VIAPMPPIIH APRINVVPMP PAAPPIMAPR PPPMIVPTAF VPAPPVAPVP 

       670        680        690        700        710        720 
APAPMPPVHP PPPMEDEPPS KKLKTEDSLM PEEEFLRRNK GPVSIKVQVP NMQDKTEWKL 

       730        740        750        760        770        780 
NGQGLVFTLP LTDQVSVIKV KIHEATGMPA GKQKLQYEGI FIKDSNSLAY YNMASGAVIH 

       790 
LALKERGGRK K 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Skin and Testis.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary gland and Mammary tumor.
[4]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 238-246; 471-484 AND 755-763, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[5]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[6]"Solution structure of ubiquitin-like domain in Sf3a120."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 685-786.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK028829 mRNA. Translation: BAC26142.1.
AK029095 mRNA. Translation: BAC26294.1.
AK030223 mRNA. Translation: BAC26853.1.
AL807825 Genomic DNA. Translation: CAI25745.1.
BC010727 mRNA. Translation: AAH10727.1.
BC029753 mRNA. Translation: AAH29753.1.
CCDSCCDS24380.1.
RefSeqNP_080451.4. NM_026175.5.
UniGeneMm.156914.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WE7NMR-A685-786[»]
ProteinModelPortalQ8K4Z5.
SMRQ8K4Z5. Positions 48-110, 134-288, 685-788.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid212207. 36 interactions.
IntActQ8K4Z5. 37 interactions.
MINTMINT-1868050.

PTM databases

PhosphoSiteQ8K4Z5.

Proteomic databases

MaxQBQ8K4Z5.
PaxDbQ8K4Z5.
PRIDEQ8K4Z5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002198; ENSMUSP00000002198; ENSMUSG00000002129.
GeneID67465.
KEGGmmu:67465.
UCSCuc007hup.2. mouse.

Organism-specific databases

CTD10291.
MGIMGI:1914715. Sf3a1.

Phylogenomic databases

eggNOGNOG300902.
GeneTreeENSGT00730000111077.
HOGENOMHOG000238941.
HOVERGENHBG059993.
InParanoidQ8K4Z5.
KOK12825.
OMAVMQQQQT.
OrthoDBEOG7JDQX9.
PhylomeDBQ8K4Z5.
TreeFamTF105705.

Gene expression databases

BgeeQ8K4Z5.
CleanExMM_SF3A1.
GenevestigatorQ8K4Z5.

Family and domain databases

InterProIPR022030. PRP21-like.
IPR000061. Surp.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF12230. PRP21_like_P. 1 hit.
PF01805. Surp. 2 hits.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTSM00648. SWAP. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMSSF109905. SSF109905. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEPS50128. SURP. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSF3A1. mouse.
EvolutionaryTraceQ8K4Z5.
NextBio324654.
PROQ8K4Z5.
SOURCESearch...

Entry information

Entry nameSF3A1_MOUSE
AccessionPrimary (citable) accession number: Q8K4Z5
Secondary accession number(s): Q8C0M7 expand/collapse secondary AC list , Q8C128, Q8C175, Q921T3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: October 1, 2002
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot