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Q8K4Z5

- SF3A1_MOUSE

UniProt

Q8K4Z5 - SF3A1_MOUSE

Protein

Splicing factor 3A subunit 1

Gene

Sf3a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei169 – 1691Critical for binding to SF3A3By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: UniProtKB

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Splicing factor 3A subunit 1
    Alternative name(s):
    SF3a120
    Gene namesi
    Name:Sf3a1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1914715. Sf3a1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: Ensembl
    2. U2-type spliceosomal complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 791791Splicing factor 3A subunit 1PRO_0000114918Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551N6-acetyllysine1 Publication
    Modified residuei320 – 3201PhosphoserineBy similarity
    Modified residuei329 – 3291PhosphoserineBy similarity
    Modified residuei357 – 3571PhosphoserineBy similarity
    Modified residuei411 – 4111PhosphoserineBy similarity
    Modified residuei449 – 4491PhosphoserineBy similarity
    Modified residuei454 – 4541PhosphotyrosineBy similarity
    Modified residuei757 – 7571PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8K4Z5.
    PaxDbiQ8K4Z5.
    PRIDEiQ8K4Z5.

    PTM databases

    PhosphoSiteiQ8K4Z5.

    Expressioni

    Gene expression databases

    BgeeiQ8K4Z5.
    CleanExiMM_SF3A1.
    GenevestigatoriQ8K4Z5.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex By similarity. Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts with SF3A3 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi212207. 36 interactions.
    IntActiQ8K4Z5. 37 interactions.
    MINTiMINT-1868050.

    Structurei

    Secondary structure

    1
    791
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi685 – 6873
    Helixi692 – 6987
    Beta strandi703 – 7097
    Beta strandi713 – 7153
    Beta strandi721 – 7299
    Helixi736 – 74510
    Turni750 – 7523
    Beta strandi753 – 7575
    Beta strandi760 – 7623
    Helixi768 – 7714
    Beta strandi778 – 7836

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WE7NMR-A685-786[»]
    ProteinModelPortaliQ8K4Z5.
    SMRiQ8K4Z5. Positions 48-110, 134-288, 685-788.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8K4Z5.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati52 – 9443SURP motif 1Add
    BLAST
    Repeati166 – 20843SURP motif 2Add
    BLAST
    Domaini705 – 78884Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi10 – 145Poly-Pro
    Compositional biasi118 – 1225Poly-Gln
    Compositional biasi260 – 2678Poly-Glu
    Compositional biasi367 – 3704Poly-Pro
    Compositional biasi555 – 5584Poly-Pro
    Compositional biasi670 – 6734Poly-Pro

    Domaini

    SURP motif 2 mediates direct binding to SF3A3.By similarity

    Sequence similaritiesi

    Contains 2 SURP motif repeats.PROSITE-ProRule annotation
    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG300902.
    GeneTreeiENSGT00730000111077.
    HOGENOMiHOG000238941.
    HOVERGENiHBG059993.
    InParanoidiQ8K4Z5.
    KOiK12825.
    OMAiVMQQQQT.
    OrthoDBiEOG7JDQX9.
    PhylomeDBiQ8K4Z5.
    TreeFamiTF105705.

    Family and domain databases

    InterProiIPR022030. PRP21-like.
    IPR000061. Surp.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF12230. PRP21_like_P. 1 hit.
    PF01805. Surp. 2 hits.
    PF00240. ubiquitin. 1 hit.
    [Graphical view]
    SMARTiSM00648. SWAP. 2 hits.
    SM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF109905. SSF109905. 2 hits.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50128. SURP. 2 hits.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8K4Z5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAGPVQAVP PPPPVATESK QPIEEEASSK EDPTPSKPVV GIIYPPPEVR    50
    NIVDKTASFV ARNGPEFEAR IRQNEINNPK FNFLNPNDPY HAYYRHKVSE 100
    FKEGKAQEPS AAIPKVMQQQ QQATQQQLPQ KVQAQVIQET IVPKEPPPEF 150
    EFIADPPSIS AFDLDVVKLT AQFVARNGRQ FLTQLMQKEQ RNYQFDFLRP 200
    QHSLFNYFTK LVEQYTKILI PPKGLFSKLK KEAENPREVL DQVCYRVEWA 250
    KFQERERKKE EEEKEKERVA YAQIDWHDFV VVETVDFQPN EQGNFPPPTT 300
    PEELGARILI QERYEKFGES EEVEMEVESD EEDQEKAEET PSQLDQDTQV 350
    QDMDEGSDDE EEGQKVPPPP ETPMPPPLPP TPDQVIVRKD YDPKASKPLP 400
    PAPAPDEYLV SPITGEKIPA SKMQEHMRIG LLDPRWLEQR DRSIREKQSD 450
    DEVYAPGLDI ESSLKQLAER RTDIFGVEET AIGKKIGEEE IQKPEEKVTW 500
    DGHSGSMART QQAAQANITL QEQIEAIHKA KGLVPEDDTK EKIGPSKPNE 550
    IPQQPPPPSS ATNIPSSAPP ITSVPRPPAM PPPVRTTVVS AVPVMPRPPM 600
    ASVVRLPPGS VIAPMPPIIH APRINVVPMP PAAPPIMAPR PPPMIVPTAF 650
    VPAPPVAPVP APAPMPPVHP PPPMEDEPPS KKLKTEDSLM PEEEFLRRNK 700
    GPVSIKVQVP NMQDKTEWKL NGQGLVFTLP LTDQVSVIKV KIHEATGMPA 750
    GKQKLQYEGI FIKDSNSLAY YNMASGAVIH LALKERGGRK K 791
    Length:791
    Mass (Da):88,545
    Last modified:October 1, 2002 - v1
    Checksum:iD83D0432469C3708
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti257 – 2571R → G in BAC26142. (PubMed:16141072)Curated
    Sequence conflicti368 – 3681P → L in BAC26294. (PubMed:16141072)Curated
    Sequence conflicti708 – 7081Q → L in BAC26853. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK028829 mRNA. Translation: BAC26142.1.
    AK029095 mRNA. Translation: BAC26294.1.
    AK030223 mRNA. Translation: BAC26853.1.
    AL807825 Genomic DNA. Translation: CAI25745.1.
    BC010727 mRNA. Translation: AAH10727.1.
    BC029753 mRNA. Translation: AAH29753.1.
    CCDSiCCDS24380.1.
    RefSeqiNP_080451.4. NM_026175.5.
    UniGeneiMm.156914.

    Genome annotation databases

    EnsembliENSMUST00000002198; ENSMUSP00000002198; ENSMUSG00000002129.
    GeneIDi67465.
    KEGGimmu:67465.
    UCSCiuc007hup.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK028829 mRNA. Translation: BAC26142.1 .
    AK029095 mRNA. Translation: BAC26294.1 .
    AK030223 mRNA. Translation: BAC26853.1 .
    AL807825 Genomic DNA. Translation: CAI25745.1 .
    BC010727 mRNA. Translation: AAH10727.1 .
    BC029753 mRNA. Translation: AAH29753.1 .
    CCDSi CCDS24380.1.
    RefSeqi NP_080451.4. NM_026175.5.
    UniGenei Mm.156914.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WE7 NMR - A 685-786 [» ]
    ProteinModelPortali Q8K4Z5.
    SMRi Q8K4Z5. Positions 48-110, 134-288, 685-788.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 212207. 36 interactions.
    IntActi Q8K4Z5. 37 interactions.
    MINTi MINT-1868050.

    PTM databases

    PhosphoSitei Q8K4Z5.

    Proteomic databases

    MaxQBi Q8K4Z5.
    PaxDbi Q8K4Z5.
    PRIDEi Q8K4Z5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002198 ; ENSMUSP00000002198 ; ENSMUSG00000002129 .
    GeneIDi 67465.
    KEGGi mmu:67465.
    UCSCi uc007hup.2. mouse.

    Organism-specific databases

    CTDi 10291.
    MGIi MGI:1914715. Sf3a1.

    Phylogenomic databases

    eggNOGi NOG300902.
    GeneTreei ENSGT00730000111077.
    HOGENOMi HOG000238941.
    HOVERGENi HBG059993.
    InParanoidi Q8K4Z5.
    KOi K12825.
    OMAi VMQQQQT.
    OrthoDBi EOG7JDQX9.
    PhylomeDBi Q8K4Z5.
    TreeFami TF105705.

    Miscellaneous databases

    ChiTaRSi SF3A1. mouse.
    EvolutionaryTracei Q8K4Z5.
    NextBioi 324654.
    PROi Q8K4Z5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8K4Z5.
    CleanExi MM_SF3A1.
    Genevestigatori Q8K4Z5.

    Family and domain databases

    InterProi IPR022030. PRP21-like.
    IPR000061. Surp.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF12230. PRP21_like_P. 1 hit.
    PF01805. Surp. 2 hits.
    PF00240. ubiquitin. 1 hit.
    [Graphical view ]
    SMARTi SM00648. SWAP. 2 hits.
    SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF109905. SSF109905. 2 hits.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50128. SURP. 2 hits.
    PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Skin and Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland and Mammary tumor.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 238-246; 471-484 AND 755-763, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-55, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    6. "Solution structure of ubiquitin-like domain in Sf3a120."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 685-786.

    Entry informationi

    Entry nameiSF3A1_MOUSE
    AccessioniPrimary (citable) accession number: Q8K4Z5
    Secondary accession number(s): Q8C0M7
    , Q8C128, Q8C175, Q921T3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3