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Protein

Beta-2 adrenergic receptor

Gene

Adrb2

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113Agonist or antagonistBy similarity1
Binding sitei118Agonist or antagonistBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-2 adrenergic receptor
Alternative name(s):
Beta-2 adrenoreceptor
Short name:
Beta-2 adrenoceptor
Gene namesi
Name:Adrb2
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein By similarity
  • Early endosome By similarity

  • Note: Colocalizes with VHL on cell membranes. Activated receptors are internalized into endosomes prior to their degradation in lysosomes.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37ExtracellularBy similarityAdd BLAST37
Transmembranei38 – 58Helical; Name=1By similarityAdd BLAST21
Topological domaini59 – 70CytoplasmicBy similarityAdd BLAST12
Transmembranei71 – 91Helical; Name=2By similarityAdd BLAST21
Topological domaini92 – 107ExtracellularBy similarityAdd BLAST16
Transmembranei108 – 128Helical; Name=3By similarityAdd BLAST21
Topological domaini129 – 151CytoplasmicBy similarityAdd BLAST23
Transmembranei152 – 172Helical; Name=4By similarityAdd BLAST21
Topological domaini173 – 197ExtracellularBy similarityAdd BLAST25
Transmembranei198 – 218Helical; Name=5By similarityAdd BLAST21
Topological domaini219 – 274CytoplasmicBy similarityAdd BLAST56
Transmembranei275 – 295Helical; Name=6By similarityAdd BLAST21
Topological domaini296 – 306ExtracellularBy similarityAdd BLAST11
Transmembranei307 – 327Helical; Name=7By similarityAdd BLAST21
Topological domaini328 – 418CytoplasmicBy similarityAdd BLAST91

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5414.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003051751 – 418Beta-2 adrenergic receptorAdd BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Glycosylationi15N-linked (GlcNAc...)Sequence analysis1
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi106 ↔ 191PROSITE-ProRule annotation
Modified residuei141PhosphotyrosineBy similarity1
Disulfide bondi184 ↔ 190PROSITE-ProRule annotation
Modified residuei246PhosphoserineBy similarity1
Modified residuei261Phosphoserine; by PKASequence analysis1
Modified residuei262Phosphoserine; by PKASequence analysis1
Lipidationi341S-palmitoyl cysteineBy similarity1
Modified residuei345Phosphoserine; by PKABy similarity1
Modified residuei355Phosphoserine; by BARKBy similarity1
Modified residuei356Phosphoserine; by BARKBy similarity1
Modified residuei3874-hydroxyprolineBy similarity1
Modified residuei4004-hydroxyprolineBy similarity1

Post-translational modificationi

Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.By similarity
Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent (By similarity).By similarity
Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Binds SLC9A3R1 and GPRASP1. Interacts with ARRB1 and ARRB2. Interacts with SRC, USP20 and USP33. Interacts with VHL; the interaction, which is increased on hydroxylation of ADRB2, ubiquitinates ADRB2 leading to its degradation. Interacts with EGLN3; the interaction hydroxylates ADRB2 facilitating VHL-E3 ligase-mediated ubiquitination. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane. Interacts with CNIH4. Interacts with ARRDC3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000018242.

Chemistry databases

BindingDBiQ8K4Z4.

Structurei

3D structure databases

ProteinModelPortaliQ8K4Z4.
SMRiQ8K4Z4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni193 – 207Agonist and antagonist bindingBy similarityAdd BLAST15
Regioni286 – 293Agonist and antagonist bindingBy similarity8
Regioni312 – 316Agonist and antagonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi415 – 418PDZ-binding4

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ8K4Z4.
OMAiGRFHAQN.
OrthoDBiEOG091G06VI.
TreeFamiTF316350.

Family and domain databases

InterProiView protein in InterPro
IPR002233. ADR_fam.
IPR000332. ADRB2_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PANTHERiPTHR24248:SF97. PTHR24248:SF97. 1 hit.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR01103. ADRENERGICR.
PR00562. ADRENRGCB2AR.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8K4Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHLGNGSDF LLAPNASHAP DHNVTRERDE AWVVGMAIVM SLIVLAIVFG
60 70 80 90 100
NVLVITAIAK FERLQTVTNY FITSLACADL VMGLAVVPFG ASHILMNMWT
110 120 130 140 150
FGNFWCEFWT SIDVLCVTAS IETLCVIAVD RYFAITSPFK YQSLLTKNKA
160 170 180 190 200
RVVILMVWVV SGLTSFLPIQ MHWYRATHKD AINCYAEETC CDFFTNQAYA
210 220 230 240 250
IASSIVSFYL PLVVMVFVYS RVFQVAKKQL QKIDRSEGRF HTQNLSQVEQ
260 270 280 290 300
DGRSGHGLRR SSKFYLKEHK ALKTLGIIMG TFTLCWLPFF IVNIVHVIQD
310 320 330 340 350
NLIPKEVYIL LNWVGYVNSA FNPLIYCRSP DFRIAFQELL CLRRSALKAY
360 370 380 390 400
GNDCSSNSNG KTDYTGEPNV CHQGQEKERE LLCEDPPGTE DLVSCPGTVP
410
SDSIDSQGRN YSTNDSLL
Length:418
Mass (Da):46,991
Last modified:October 1, 2002 - v1
Checksum:i1090D41C96F00E50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ459814 Genomic DNA. Translation: CAD30869.1.
RefSeqiXP_003477383.1. XM_003477335.3.

Genome annotation databases

EnsembliENSCPOT00000012875; ENSCPOP00000018242; ENSCPOG00000012753.
GeneIDi100722663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ459814 Genomic DNA. Translation: CAD30869.1.
RefSeqiXP_003477383.1. XM_003477335.3.

3D structure databases

ProteinModelPortaliQ8K4Z4.
SMRiQ8K4Z4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000018242.

Chemistry databases

BindingDBiQ8K4Z4.
ChEMBLiCHEMBL5414.

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCPOT00000012875; ENSCPOP00000018242; ENSCPOG00000012753.
GeneIDi100722663.

Organism-specific databases

CTDi154.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiQ8K4Z4.
OMAiGRFHAQN.
OrthoDBiEOG091G06VI.
TreeFamiTF316350.

Miscellaneous databases

PROiQ8K4Z4.

Family and domain databases

InterProiView protein in InterPro
IPR002233. ADR_fam.
IPR000332. ADRB2_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PANTHERiPTHR24248:SF97. PTHR24248:SF97. 1 hit.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR01103. ADRENERGICR.
PR00562. ADRENRGCB2AR.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADRB2_CAVPO
AccessioniPrimary (citable) accession number: Q8K4Z4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2002
Last modified: March 15, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.